CAJ | 학술논문

目的分析GⅡ-4型诺如病毒深圳分离株SZ2010422全基因组序列,了解其分子结构特点及进化特性.方法根据New Orleans参考株全序列设计引物,用RT-PCR分段扩增诺如病毒全基因组,经分子克隆、测序后进行系统进化分析及衣壳区氨基酸位点分析.结果 GⅡ-4型诺如病毒深圳SZ2010422株病毒基因组全长7559 bp,病毒基因组分三个开放阅读框(ORFs),ORF1、ORF2及ORF3长度分别为5100 bp、1623 bp和807 bp,ORF1与ORF2之间有19个核苷酸重叠.深圳株SZ2010422基因组核苷酸序列与参考株GⅡ-4型New Orleans变异株同源性最高,全长同源性为99.3％,ORFI、ORF2、ORF3同源性分别为99.5％、99.2％和98.6％.根据系统进化分析,深圳SZ2010422株属于GⅡ-4 New Orleans变异株.通过对衣壳区氨基酸比对,New Orleans变异株位点变化情况为:aa310N或K→S,aa341D→N,aa359T→S,aa396H→P,aa460H→Y.结论诺如病毒深圳株SZ2010422属于GⅡ-4型New Orleans变异株.
목적 분석GⅡ-4형낙여병독심수분리주SZ2010422전기인조서렬,료해기분자결구특점급진화특성.방법 근거New Orleans삼고주전서렬설계인물,용RT-PCR분단확증낙여병독전기인조,경분자극륭、측서후진행계통진화분석급의각구안기산위점분석.결과 GⅡ-4형낙여병독심수SZ2010422주병독기인조전장7559 bp,병독기인조분삼개개방열독광(ORFs),ORF1、ORF2급ORF3장도분별위5100 bp、1623 bp화807 bp,ORF1여ORF2지간유19개핵감산중첩.심수주SZ2010422기인조핵감산서렬여삼고주GⅡ-4형New Orleans변이주동원성최고,전장동원성위99.3％,ORFI、ORF2、ORF3동원성분별위99.5％、99.2％화98.6％.근거계통진화분석,심수SZ2010422주속우GⅡ-4 New Orleans변이주.통과대의각구안기산비대,New Orleans변이주위점변화정황위:aa310N혹K→S,aa341D→N,aa359T→S,aa396H→P,aa460H→Y.결론 낙여병독심수주SZ2010422속우GⅡ-4형New Orleans변이주.
Objective To obtain information on viral molecular structural and evolutionary characteristics,we conducted the SZ2010422 full-length genomic analysis.Methods Primers were designed by New Orleans full sequence,SZ2010422 full genome was amplified by RT-PCR,the whole genome sequence and the capsid domain amino acid sites was analysised after cloned and sequenced.Results The genome of G lⅡ-4 Norovirus SZ2010422 strain was consist of 7559 bp,it revealed three ORFs composites of the whole genome,ORF1 (5100 bp),ORF2 (1623 bp),ORF3 (807 bp) respectively,ORF1 and ORF2 had 19 nucleotide overlap.By evolutionary comparative analysis found SZ2010422 genomic nucleotide sequences with reference strains of G Ⅱ-4 New Orleansl805 strains the highest homology with a total length of homology was 99.3％,of ORF1 (99.5％),ORF2 (99.2％),ORF3 (98.6％).Phylogenetic analyses showed SZ2010422 belonging to G Ⅱ-4 New Orleans variant.Date of 541 amino acid analyses showed:New Orleans variant strains of popular sites:aa310N or K,→S aa341D→of N,aa359T→S,aa396H→P,aa460H→Y.Conclusion Norovirus SZ2010422 belonged to the G Ⅱ-4 New Orleans variant.In This study,SZ2010422 full sequence can be used not only as a full-length NoV variant sequence standard for future comparison studies,but also as useful material for the public health field by enabling the diagnosis,vaccine development,and prediction of new emerging variants.