CAJ | 학술논문

目的了解2008-2009年北京地区成人腹泻样本中诺如病毒(Norovirus,NoV)GⅡ.12型毒株的基因特征.方法用三对引物对11株GⅡ.12型诺如病毒毒株RdRp,、ORF2、ORF3及ORF1/OFF2重叠区分别扩增,PCR产物纯化、克隆、测序,通过DNAStar、MEGA、SimPlot等生物软件进行比对、系统进化分析及重组分析.结果根据系统进化分析,11株在RdRp区属于GⅡ.g,在ORF2和ORF3区均属于GⅡ.12.SimPlot分析进一步证实了11株均为GⅡ.g/GⅡ.12重组株.结论北京地区流行的GⅡ.12型诺如病毒与世界其他地区流行的GⅡ.12型诺如病毒毒株均属同一毒株,确定了北京地区GⅡ.12型诺如病毒毒株的基因特征.
목적 료해2008-2009년북경지구성인복사양본중낙여병독(Norovirus,NoV)GⅡ.12형독주적기인특정.방법 용삼대인물대11주GⅡ.12형낙여병독독주RdRp,、ORF2、ORF3급ORF1/OFF2중첩구분별확증,PCR산물순화、극륭、측서,통과DNAStar、MEGA、SimPlot등생물연건진행비대、계통진화분석급중조분석.결과 근거계통진화분석,11주재RdRp구속우GⅡ.g,재ORF2화ORF3구균속우GⅡ.12.SimPlot분석진일보증실료11주균위GⅡ.g/GⅡ.12중조주.결론 북경지구류행적GⅡ.12형낙여병독여세계기타지구류행적GⅡ.12형낙여병독독주균속동일독주,학정료북경지구GⅡ.12형낙여병독독주적기인특정.
Objective To reveal the genetic characteristics of G Ⅱ.12 Norovirus strains isolating from stool samples of adults with diarrhea in Beijing during 2008-2009.Methods RdRp,ORF2,ORF3 and ORF1/ORF2 overlap region were respectively amplified by primers using RT-PCR.The products were purified,cloned,sequenced and then aligned,phylogenetie and recombinant analyzed by softwares of DNAStar,M EGA and SimPlot.Results According to the phylogenetic analysis,11 strains belonged to G Ⅱ.g in the RdRp region,while GⅡ.12 in the ORF2 and ORF3.SimPlot analysis further confirmed the 11 strains were recombinant strains (G Ⅱ.g [RdRp]/G Ⅱ.12 [capsid]).Conclusion G Ⅱ.12 Norovirus prevailing in Beijing and other regions of the world belonged to the same strain,and we identified the genetic characteristics of G Ⅱ.12 Norovirus in Beijing.