CAJ | 학술논문

目的检测乙肝相关性肝衰竭患者血清中IL-32表达,探讨IL-32水平与丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)、总胆红素(TBIL)及HBV DNA载量的相关性.方法 TRIzol提取55例乙肝相关性肝衰竭患者(重肝组)及20例健康人(对照组)外周血中淋巴细胞总RNA,real-time PCR检测IL-32 mRNA表达水平；用酶联免疫吸附法(ELISA)检测血清中IL-32蛋白表达,并与患者ALT、AST、TBil、HBV DNA两组之间的差异有统计学意义(P＜0.05).结果 IL-32水平与ALT、AST、TBil呈正相关(P＜0.05),与HBV DNA载量无相关性(P＞0.05).结论乙肝相关性肝衰竭患者血清中IL-32表达增高,与炎症程度相关,可能参与乙肝相关性肝衰竭的发病.
목적 검측을간상관성간쇠갈환자혈청중IL-32표체,탐토IL-32수평여병안산전안매(ALT)、천동안산전안매(AST)、총담홍소(TBIL)급HBV DNA재량적상관성.방법 TRIzol제취55례을간상관성간쇠갈환자(중간조)급20례건강인(대조조)외주혈중림파세포총RNA,real-time PCR검측IL-32 mRNA표체수평；용매련면역흡부법(ELISA)검측혈청중IL-32단백표체,병여환자ALT、AST、TBil、HBV DNA량조지간적차이유통계학의의(P＜0.05).결과 IL-32수평여ALT、AST、TBil정정상관(P＜0.05),여HBV DNA재량무상관성(P＞0.05).결론 을간상관성간쇠갈환자혈청중IL-32표체증고,여염증정도상관,가능삼여을간상관성간쇠갈적발병.
Objective To investigate the expression level of IL-32 in serum and its correlation with serum biochemical indices of liver function test and HBV DNA load in patients with HBV-related liver failure.Methods Fifty-five patients with HBV-related liver failure (severe hepatitis group) and twenty normal cases (control group) were enrolled in the study.Total RNA in PBMCs was extracted by using TRIzol.IL-32 mRNA level was assayed by using Real-time PCR.IL-32 protein level in serum was detected by ELSIA method.The correlation between IL-32 and ALT,AST,TBIL,HBV DNA load was analyzed using pearson's correlation analysis,respectively.Results Serum IL-32 expression level in severe hepatitis group was higher than that of control group.Moreover,the difference between them was statistically significant (P ＜0.05).Serum IL-32 level was positively correlated with serum ALT,AST,TBIL,respectively (P ＜ 0.05),but was not correlated with HBV DNA load (P ＞ 0.05).Conclusion Serum IL-32 expression level was increased in patients with HBV-related liver failure and was associated with the severity of inflammation.We,therefore,believe that IL-32 might be involved in the pathogenesis of HBV-related liver failure.