中华实验和临床病毒学杂志
中華實驗和臨床病毒學雜誌
중화실험화림상병독학잡지
CHINESE JOURNAL OF EXPERIMENTAL AND CLINICAL VIROLOGY
2013年
4期
250-252
,共3页
谢放%张超%朱跃科%刘晖%刘雪梅%贾琳%王克菲%陈德喜%孟庆华
謝放%張超%硃躍科%劉暉%劉雪梅%賈琳%王剋菲%陳德喜%孟慶華
사방%장초%주약과%류휘%류설매%가림%왕극비%진덕희%맹경화
肝功能衰竭%显微镜检查,电子%聚合酶链反应
肝功能衰竭%顯微鏡檢查,電子%聚閤酶鏈反應
간공능쇠갈%현미경검사,전자%취합매련반응
Liver failure%Microscopy,electrol%Polymenase chain reaction
目的 研究泛醌是否能通过调节自噬减轻慢加急肝衰竭大鼠肝损伤.方法 取空白对照、慢加急性肝衰竭模型组和慢加急性肝衰竭干预组(造模同时增加泛醌植物油每天注射10 mg/kg灌胃6周)SD大鼠各10只,光镜观察三组肝组织病理改变,电镜观察三组肝组织中线粒体超微结构,荧光定量PCR检测三组自噬基因Beclin-1 mRNA表达,Western Blot检测三组Beclin-1蛋白表达.结果 光镜下干预组肝细胞坏死面积约80％,模型组坏死面积约100％.电镜下模型组大部分线粒体内容物已经降解,而干预组多数线粒体尚存.荧光定量结果显示干预组Beclin-1表达水平较模型组显著升高(P＜0.01).结论 泛醌可能通过促进自噬减轻慢加急性肝衰竭大鼠肝损伤.
目的 研究汎醌是否能通過調節自噬減輕慢加急肝衰竭大鼠肝損傷.方法 取空白對照、慢加急性肝衰竭模型組和慢加急性肝衰竭榦預組(造模同時增加汎醌植物油每天註射10 mg/kg灌胃6週)SD大鼠各10隻,光鏡觀察三組肝組織病理改變,電鏡觀察三組肝組織中線粒體超微結構,熒光定量PCR檢測三組自噬基因Beclin-1 mRNA錶達,Western Blot檢測三組Beclin-1蛋白錶達.結果 光鏡下榦預組肝細胞壞死麵積約80％,模型組壞死麵積約100％.電鏡下模型組大部分線粒體內容物已經降解,而榦預組多數線粒體尚存.熒光定量結果顯示榦預組Beclin-1錶達水平較模型組顯著升高(P＜0.01).結論 汎醌可能通過促進自噬減輕慢加急性肝衰竭大鼠肝損傷.
목적 연구범곤시부능통과조절자서감경만가급간쇠갈대서간손상.방법 취공백대조、만가급성간쇠갈모형조화만가급성간쇠갈간예조(조모동시증가범곤식물유매천주사10 mg/kg관위6주)SD대서각10지,광경관찰삼조간조직병리개변,전경관찰삼조간조직중선립체초미결구,형광정량PCR검측삼조자서기인Beclin-1 mRNA표체,Western Blot검측삼조Beclin-1단백표체.결과 광경하간예조간세포배사면적약80％,모형조배사면적약100％.전경하모형조대부분선립체내용물이경강해,이간예조다수선립체상존.형광정량결과현시간예조Beclin-1표체수평교모형조현저승고(P＜0.01).결론 범곤가능통과촉진자서감경만가급성간쇠갈대서간손상.
Objective To study whether CO-Q10 can protect liver injury caused by acute on chronic liver failure(ACLF) by autophagy.Methods Rats were separated into three groups:control group,acute on chronic liver failure (ACLF) and intervenient group,liver tissues were observed by optical microscopy and electron microscopy.The levels of Beclin-1 expression were determined by real-time PCR.And Western Blot.Results Areas of necrosis detected in intervenient group were alleviated than in ACLF significantly.Most mitochondrias had been degradated in ACLF group while alive in intervenient group.Real-time PCR and Western Blot revealed level of beclin-1 in ACLF was lower than control and intervenient group.Conclusion lntervenient group may ameliorate rat liver injury by promoting autophagy.
