CAJ | 학술논문

目的对青海省2000-2011年流行麻疹野病毒进行分子流行病学研究,为消除麻疹提供科学依据.方法使用B95a细胞或Vero/SLAM细胞从2000-2011年青海省6个地市麻疹暴发和散发病例的咽拭子标本中分离的麻疹病毒,用RT-PCR方法扩增N基因羧基末端676个核苷酸片段,再对扩增产物进行核苷酸序列测定和分析,并与我国其他省份流行的麻疹病毒代表株基于N基因羧基末端450个核苷酸片段构建基因亲缘性关系树.结果青海省2000-2011年共分离到19株麻疹病毒,均为H1基因型中的Hla基因亚型.在亲缘性关系树中显示,2000-2005年间流行的麻疹毒株与2009-2011年间流行的麻疹毒株处在两个不同的簇中,提示19株麻疹病毒株至少属于2条病毒传播链,其中2000-2005年间流行的麻疹病毒之后未再监测到.结论青海省2000-2011年间流行的麻疹野病毒以Hla为绝对优势基因亚型,未监测到其他基因型病毒.青海省麻疹病毒不是独立进化的,而是与我国流行的麻疹病毒在共同进化.
목적 대청해성2000-2011년류행마진야병독진행분자류행병학연구,위소제마진제공과학의거.방법 사용B95a세포혹Vero/SLAM세포종2000-2011년청해성6개지시마진폭발화산발병례적인식자표본중분리적마진병독,용RT-PCR방법확증N기인최기말단676개핵감산편단,재대확증산물진행핵감산서렬측정화분석,병여아국기타성빈류행적마진병독대표주기우N기인최기말단450개핵감산편단구건기인친연성관계수.결과 청해성2000-2011년공분리도19주마진병독,균위H1기인형중적Hla기인아형.재친연성관계수중현시,2000-2005년간류행적마진독주여2009-2011년간류행적마진독주처재량개불동적족중,제시19주마진병독주지소속우2조병독전파련,기중2000-2005년간류행적마진병독지후미재감측도.결론 청해성2000-2011년간류행적마진야병독이Hla위절대우세기인아형,미감측도기타기인형병독.청해성마진병독불시독립진화적,이시여아국류행적마진병독재공동진화.
Objective To carry out the molecular epidemiological study of the wild-type measles virus isolated in Qinghai Province during 2000-2011,and provide a scientific basis for the measles elimination.Methods Measles viruses were isolated using B95a cell line or Vero/SLAM cell line from throat swabs collected from suspected measles cases during measles outbreak and sporadic in 6 prefectures during 2000-2011.The fragment of 696 nucleotides of N gene carboxy terminal was amplified by using RTPCR methods.The PCR products were sequenced and analyzed.The phylogenetic tree was conducted with the viruses isolated in viruses from other province.Results Total 19 measles viruses were isolated during 2000-2011 in Qinghai province and all belong to genotype H1 a.The results of phylogenetic tree showed that viruses in 2000-2005 and in 2009-2011 were distributed in two different lineages,and it revealed that these strains belonged to at least 2 viral transmission chains and the viruses circulated during 2000-2005 were not detected after 2005.Conclusion Genotype H1a was the predominant genotype circulated in Qinghai province during 2000-2011.Qinghai measles virus strains had not evolved independently,but coevolved with the measles virus strains in other provinces in mainland China.The variation of important amino acid sites of measles virus should be continuous monitored and provide the scientific strategy for the measles elimination.