中华实验和临床病毒学杂志
中華實驗和臨床病毒學雜誌
중화실험화림상병독학잡지
CHINESE JOURNAL OF EXPERIMENTAL AND CLINICAL VIROLOGY
2013年
4期
292-294
,共3页
赵静%马洪滨%侯俊%郭静霞%徐军%宋永继%陈霖%刘爱霞%刘佳
趙靜%馬洪濱%侯俊%郭靜霞%徐軍%宋永繼%陳霖%劉愛霞%劉佳
조정%마홍빈%후준%곽정하%서군%송영계%진림%류애하%류가
酶联免疫吸附测定%肝炎病毒,乙型%病毒包膜蛋白质类
酶聯免疫吸附測定%肝炎病毒,乙型%病毒包膜蛋白質類
매련면역흡부측정%간염병독,을형%병독포막단백질류
Enzyme-linked immunosorbent assay%Hepatitis B virus%Viral envilope proteins
目的 建立人血清中乙型肝炎病毒外膜大蛋白(HBV-LP)的酶联免疫吸附试验(ELISA)检测方法.方法 辣根过氧化物酶标记HBV-LP单抗,优化各类反应液的工作浓度和各种反应条件,建立双抗体夹心的ELISA检测方法；同时评价所建立方法的灵敏度、特异性、稳定性等性能指标.结果 所建立方法的灵敏度为5 ng/ml,批内、批间变异均小于10％.在4℃和37℃条件下分别进行了3、5、7d的稳定性考察,线性相关系数均＞0.98,标准偏差＜10％.结论 成功建立了血清中HBV-LP的检测方法,该方法性能能够满足临床检测的需求.
目的 建立人血清中乙型肝炎病毒外膜大蛋白(HBV-LP)的酶聯免疫吸附試驗(ELISA)檢測方法.方法 辣根過氧化物酶標記HBV-LP單抗,優化各類反應液的工作濃度和各種反應條件,建立雙抗體夾心的ELISA檢測方法；同時評價所建立方法的靈敏度、特異性、穩定性等性能指標.結果 所建立方法的靈敏度為5 ng/ml,批內、批間變異均小于10％.在4℃和37℃條件下分彆進行瞭3、5、7d的穩定性攷察,線性相關繫數均＞0.98,標準偏差＜10％.結論 成功建立瞭血清中HBV-LP的檢測方法,該方法性能能夠滿足臨床檢測的需求.
목적 건립인혈청중을형간염병독외막대단백(HBV-LP)적매련면역흡부시험(ELISA)검측방법.방법 랄근과양화물매표기HBV-LP단항,우화각류반응액적공작농도화각충반응조건,건립쌍항체협심적ELISA검측방법；동시평개소건립방법적령민도、특이성、은정성등성능지표.결과 소건립방법적령민도위5 ng/ml,비내、비간변이균소우10％.재4℃화37℃조건하분별진행료3、5、7d적은정성고찰,선성상관계수균＞0.98,표준편차＜10％.결론 성공건립료혈청중HBV-LP적검측방법,해방법성능능구만족림상검측적수구.
Objective To establish enzyme-linked immunosorbent assay (ELISA) for detection of hepatitis B virus large surface protein(HBV-LP) in serum.Methods A sandwich reaction was preformed with horseradish peroxidase labeled monoclonal antibody of HBV-LP as the catalytic enzyme.Several reactions liquid's concentration and reaction conditions were optimized.The method was evaluated in all aspects such as sensitivity,specificity,stability and so on.Results The detection limit was 5 ng/ml.Interassay and intra-assay RSD were both less than 10％.After stored at 4℃ and 37℃ for 3,5,7 days,the analysis showed correlation coefficient higher than 0.98 and RSD lower than 10％.Conclusion Established ELISA for determination of serum HBV-LP has high sensitivity and repeatability.
