CAJ | 학술논문

目的探讨RNA干扰Toll样受体4(Toll-like receptor 4,TLR4)信号通路对枯否细胞(Kupffer cells,KCs)吞噬功能的影响.方法以RAW 264.7小鼠单核巨噬细胞白血病细胞为研究对象,经QPCR验证得到最优干扰RNA为Tlr4-mus-1567后,以正常细胞为对照组,Negative Control RNA 干扰为NC对照组,Tlr4-mus-1567 RNA干扰为实验组,倒置显微镜观察各组细胞吞噬酵母菌情况,并测定各组的吞噬细胞百分数和吞噬指数,组间进行比较.结果实验组平均吞噬细胞百分数较正常对照组明显降低(17.67％±3.51％和32.00％±3.00％,P＜0.01),实验组平均吞噬指数较正常对照组明显降低(46.33％ ±7.51％和82.00％±6.08％,P＜0.01).结论 RNA干扰TLR4信号通路能降低KCs的吞噬功能.
목적 탐토RNA간우Toll양수체4(Toll-like receptor 4,TLR4)신호통로대고부세포(Kupffer cells,KCs)탄서공능적영향.방법 이RAW 264.7소서단핵거서세포백혈병세포위연구대상,경QPCR험증득도최우간우RNA위Tlr4-mus-1567후,이정상세포위대조조,Negative Control RNA 간우위NC대조조,Tlr4-mus-1567 RNA간우위실험조,도치현미경관찰각조세포탄서효모균정황,병측정각조적탄서세포백분수화탄서지수,조간진행비교.결과 실험조평균탄서세포백분수교정상대조조명현강저(17.67％±3.51％화32.00％±3.00％,P＜0.01),실험조평균탄서지수교정상대조조명현강저(46.33％ ±7.51％화82.00％±6.08％,P＜0.01).결론 RNA간우TLR4신호통로능강저KCs적탄서공능.
Objective To investigate the effect of RNA interfering TLR4 signal pathway on phagocytosis of Kupffer cells.Methods RAW2647 mice mononuclear macrophage leukemia cells were observed.The tested group was interfered by Tlr4-mus-1567 RNA which had the best result confirmed by QPCR,cells interfered by Negative Control RNA as NC group,and normal cell as control.We perform the phagocytosis test on each group.Results The tested group has lower phagocytes percentage than control (17.67％ ±3.51％ vs 32.00％ ±3.00％,P＜0.01),and lower phagocytic index(46.33％ ±7.51％ vs 82.00％ ± 6.08％,P ＜0.01).Conclusions Decreased phagocytic activity was observed on Kupffer cells by RNA interference.