CAJ | 학술논문

目的观察基于枯草芽孢杆菌系统制备的EV71 VP1重组抗原诱导BALB/c小鼠产生的免疫应答反应.方法通过建立BALB/c小鼠动物模型,分别将前期构建的基于枯草芽孢杆菌系统制备的EV71重组抗原(rVP1)、灭活EV71病毒免疫组(EV71)和空白对照组(PBS),经鼻腔接种6～8周龄BALB/c小鼠,ELISA方法检测免疫后不同时间产生的特异性IgG、IgA以及IgG1和IgG2a水平.结果 EV71重组抗原可以有效诱导小鼠产生高水平的体液免疫和明显的黏膜免疫应答,且能够诱发均衡的Th1、Th2免疫调节.结论肠道病毒71型重组VP1抗原可诱发小鼠产生明显的特异性体液免疫和黏膜免疫应答.
목적 관찰기우고초아포간균계통제비적EV71 VP1중조항원유도BALB/c소서산생적면역응답반응.방법 통과건립BALB/c소서동물모형,분별장전기구건적기우고초아포간균계통제비적EV71중조항원(rVP1)、멸활EV71병독면역조(EV71)화공백대조조(PBS),경비강접충6～8주령BALB/c소서,ELISA방법검측면역후불동시간산생적특이성IgG、IgA이급IgG1화IgG2a수평.결과 EV71중조항원가이유효유도소서산생고수평적체액면역화명현적점막면역응답,차능구유발균형적Th1、Th2면역조절.결론 장도병독71형중조VP1항원가유발소서산생명현적특이성체액면역화점막면역응답.
Objective To observe the immunological effect of the Recombinant VP1 proteins of enterovirus 71 in mice.Methods VP1 protein was expressed on the bacillus subtilis surface successfully.To establishment of BALB/c mouse model,the recombinant VP1 antigen (rVP1),inactivated EV71 virus (EV71) and blank control group (PBS),were injected by intranasal inoculation in BABL/C mice,specific IgG,IgA and IgG1 and IgG2a levels were detected at different times after immunization by ELISA.Results The specific IgG and IgA were induced at higher levels in rVP1 group compared to that in other two groups,furthermore,rVP1 could induce immune balance of Th1/Th2.Conclusion Recombinant VP1 Protein could induce significant cellular and mucous immune response in mice.