中华实验和临床病毒学杂志
中華實驗和臨床病毒學雜誌
중화실험화림상병독학잡지
CHINESE JOURNAL OF EXPERIMENTAL AND CLINICAL VIROLOGY
2013年
6期
413-416
,共4页
林益敏%张国良%刘映霞%袁静%刘威龙%李建明%赵美芬%陈川铁%刘京
林益敏%張國良%劉映霞%袁靜%劉威龍%李建明%趙美芬%陳川鐵%劉京
림익민%장국량%류영하%원정%류위룡%리건명%조미분%진천철%류경
手足口病%白细胞介素IL-22%多态性,单核苷酸%飞行时间质谱
手足口病%白細胞介素IL-22%多態性,單覈苷痠%飛行時間質譜
수족구병%백세포개소IL-22%다태성,단핵감산%비행시간질보
Hand,foot and muth disease%Interleukins%Plymorphism,single nucleotide%Time of flight mass spectrum
目的 探讨Th22细胞相关性细胞因子IL-22/IL-22RA1基因单核苷酸多态性(SNP)与手足口病易感性及其转归的关系.方法 采用病例-对照研究方法,选取2012年4月至9月深圳市第三人民医院收治并确诊的手足口病住院患者296例为病例组,其中轻症手足口病患者178例,重症患者118例,对照组为同期在该医院健康体检儿童155例.应用飞行时间质谱(time of fight mass spectrum,TOF-MS)生物芯片技术,检测IL-22/IL-22RA1基因共4个SNP位点基因型(rs2227473、rs2227483、rs3795299、rs34379702),对SNP进行Hardy-Weiberg平衡检测,并计算各SNP位点在不同人群中等位基因频率,筛选与手足口病易感性、预后相关的SNP位点.结果 纳入研究的4个SNP位点的基因型分布均处于遗传平衡状态.入组人群中IL-22的基因rs2227473位点存在GG、GA和AA基因型,重症手足口病组rs222743位点A等位基因频率(13.14％,31/236)显著高于轻症组(6.18％,22/356),差异有统计学意义(x2=8.424, P＜0.001,A=2.296,95％CI=1.294-4.074).对于EV71相关性手足口病而言,重症组A等位基因频率(19.64％,22/112)显著高于轻症组(7.41％,4/54),差异有统计学意义(x2=4.129,P =0.042,A=3.056,95％CI=0.997 ～9.368).结论 IL-22基因rs2227473位点多态性与手足口病转归密切相关,尤其是EV71相关性手足口病.
目的 探討Th22細胞相關性細胞因子IL-22/IL-22RA1基因單覈苷痠多態性(SNP)與手足口病易感性及其轉歸的關繫.方法 採用病例-對照研究方法,選取2012年4月至9月深圳市第三人民醫院收治併確診的手足口病住院患者296例為病例組,其中輕癥手足口病患者178例,重癥患者118例,對照組為同期在該醫院健康體檢兒童155例.應用飛行時間質譜(time of fight mass spectrum,TOF-MS)生物芯片技術,檢測IL-22/IL-22RA1基因共4箇SNP位點基因型(rs2227473、rs2227483、rs3795299、rs34379702),對SNP進行Hardy-Weiberg平衡檢測,併計算各SNP位點在不同人群中等位基因頻率,篩選與手足口病易感性、預後相關的SNP位點.結果 納入研究的4箇SNP位點的基因型分佈均處于遺傳平衡狀態.入組人群中IL-22的基因rs2227473位點存在GG、GA和AA基因型,重癥手足口病組rs222743位點A等位基因頻率(13.14％,31/236)顯著高于輕癥組(6.18％,22/356),差異有統計學意義(x2=8.424, P＜0.001,A=2.296,95％CI=1.294-4.074).對于EV71相關性手足口病而言,重癥組A等位基因頻率(19.64％,22/112)顯著高于輕癥組(7.41％,4/54),差異有統計學意義(x2=4.129,P =0.042,A=3.056,95％CI=0.997 ～9.368).結論 IL-22基因rs2227473位點多態性與手足口病轉歸密切相關,尤其是EV71相關性手足口病.
목적 탐토Th22세포상관성세포인자IL-22/IL-22RA1기인단핵감산다태성(SNP)여수족구병역감성급기전귀적관계.방법 채용병례-대조연구방법,선취2012년4월지9월심수시제삼인민의원수치병학진적수족구병주원환자296례위병례조,기중경증수족구병환자178례,중증환자118례,대조조위동기재해의원건강체검인동155례.응용비행시간질보(time of fight mass spectrum,TOF-MS)생물심편기술,검측IL-22/IL-22RA1기인공4개SNP위점기인형(rs2227473、rs2227483、rs3795299、rs34379702),대SNP진행Hardy-Weiberg평형검측,병계산각SNP위점재불동인군중등위기인빈솔,사선여수족구병역감성、예후상관적SNP위점.결과 납입연구적4개SNP위점적기인형분포균처우유전평형상태.입조인군중IL-22적기인rs2227473위점존재GG、GA화AA기인형,중증수족구병조rs222743위점A등위기인빈솔(13.14％,31/236)현저고우경증조(6.18％,22/356),차이유통계학의의(x2=8.424, P＜0.001,A=2.296,95％CI=1.294-4.074).대우EV71상관성수족구병이언,중증조A등위기인빈솔(19.64％,22/112)현저고우경증조(7.41％,4/54),차이유통계학의의(x2=4.129,P =0.042,A=3.056,95％CI=0.997 ～9.368).결론 IL-22기인rs2227473위점다태성여수족구병전귀밀절상관,우기시EV71상관성수족구병.
Objective To explore the relationship between Th22 cell relative cytokine gene(IL-22 and IL-22RA1)polymorphism and the hand-foot-mouth disease (HFMD).Methods 296 patients in Shenzhen third people's hospital from April and September who were diagnosed as HFMD(including 178 mild cases and 118 severe eases)and 155 healthy controls were investigated using a case-control method.Total 4 SNPs,including rs2227473,rs2227483 in IL-22 gene,rs3795299,rs34379702 in IL-22RA1 gene,were genotyped using time of fight mass spectrum (TOF-MS).All the SNPs were detected whether they were in hardy-weiberg equilibrium and allele frequencies were calculated to analyze the relationship with susceptibility and outcome of HFMD.Results All the SNPs were in hardy-weinberg equilibrium.The genotypes of rs2227473 included GG,GA,and AA,and the frequency of allele A was higher in severe cases (13.14％,31/236) than that in mild cases (6.18％,22/356),there was significant difference(x2 =8.424,P＜0.001,A =2.296,95％ CI=1.294-4.074).For the HFMD infected by enterovirus 71(EV71),significant difference were also observed between severe cases (19.64％,22/112)and mild cases (7.41％,4/ 54)(x2 =4.129,P =0.042,A =3.056,95％CI=0.997 ～9.368).Conclusion IL-22 gene rs2227473 SNP was correlated with the outcomes of HFMD,especially in patients with HFMD infected by EV71.
