CAJ | 학술논문

目的探讨血清肝型脂肪酸结合蛋白在大鼠肝脏缺血再灌注损伤的早期诊断价值.方法雄性Wistar大鼠随机分为3组:假手术组(A组)；缺血15 min再灌注组(B组)；缺血30 min再灌注组(C组).建立肝缺血再灌注损伤模型,分别在缺血再灌注后15 min、1h、3h、6h、1d、3d检测血清丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)水平和肝型脂肪酸结合蛋白(L-FABP)含量；免疫组织荧光法检测肝脏L-FABP的表达；观察肝组织病理学改变并对其进行Suzuki's评分.结果与A组比较,B组血清L-FABP变化:再灌注15 min,血清L-FABP水平升高[(0.57±0.14) μg/L,P＜0.05],峰值出现于再灌注3 h[(1.70±0.26) μg/L,P＜0.05],3d基本降至正常[(0.16±0.05) μg/L,P＞0.05]；血清ALT、AST变化:再灌注15 min无明显升高,6h达高峰,3d仍高于正常(P＜0.05)；肝组织L-FABP变化:再灌注15 min表达下降(0.148 ±0.047,P＜0.05)；3h时处于谷值(0.071±0.019,P＜0.05)；3 d时基本恢复正常(0.142±0.047,P＞0.05).与B组比较,C组各时间点血清L-FABP、AST和ALT水平均明显升高(P＜0.05),肝组织L-FABP表达明显降低(P＜0.05),病理学改变明显加重.结论与传统肝功能指标(ALT、AST)比较,L-FABP是一种监测肝脏缺血再灌注损害的更为敏感的指标,与肝组织病理学变化趋势一致.
목적 탐토혈청간형지방산결합단백재대서간장결혈재관주손상적조기진단개치.방법 웅성Wistar대서수궤분위3조:가수술조(A조)；결혈15 min재관주조(B조)；결혈30 min재관주조(C조).건립간결혈재관주손상모형,분별재결혈재관주후15 min、1h、3h、6h、1d、3d검측혈청병안산안기전이매(ALT)、천동안산안기전이매(AST)수평화간형지방산결합단백(L-FABP)함량；면역조직형광법검측간장L-FABP적표체；관찰간조직병이학개변병대기진행Suzuki's평분.결과 여A조비교,B조혈청L-FABP변화:재관주15 min,혈청L-FABP수평승고[(0.57±0.14) μg/L,P＜0.05],봉치출현우재관주3 h[(1.70±0.26) μg/L,P＜0.05],3d기본강지정상[(0.16±0.05) μg/L,P＞0.05]；혈청ALT、AST변화:재관주15 min무명현승고,6h체고봉,3d잉고우정상(P＜0.05)；간조직L-FABP변화:재관주15 min표체하강(0.148 ±0.047,P＜0.05)；3h시처우곡치(0.071±0.019,P＜0.05)；3 d시기본회복정상(0.142±0.047,P＞0.05).여B조비교,C조각시간점혈청L-FABP、AST화ALT수평균명현승고(P＜0.05),간조직L-FABP표체명현강저(P＜0.05),병이학개변명현가중.결론 여전통간공능지표(ALT、AST)비교,L-FABP시일충감측간장결혈재관주손해적경위민감적지표,여간조직병이학변화추세일치.
Objective To investigate the diagnostic value and significance of serum liver fatty acid-binding protein (L-FABP) in hepatic ischemia-reperfusion injury in rat.Methods Male Wistar rats were randomly divided into three groups:sham operation group (group A) ; reperfusion after 15 min of ischemia group (group B) ; reperfusion after 30 min of ischemia group (group C).Each group was divided into 6 subgroups based on the time of reperfusion (15 min,1 h,3 h,6 h,1 d,3 d).The model of hepatic ischemia-reperfusion injury was established,the level of alanine aminotransferase (ALT),aspartate aminotransferase (AST) and L-FABP were tested at each time point and the expression of L-FABP was tested by Immunohistochemical Fluorescence.The pathological changes observed in the liver and evaluated the changes by Suzuki's scoring system.Results Compared with group A,the changes of serum L-FABP:increased after 15 min of reperfusion [(0.57 ± 0.14) μg/L,P ＜ 0.05],reached the peak after 3 h of reperfusion [(1.70 ± 0.26) μg/L,P ＜ 0.05] and returned to normal at 3 d [(0.16 ± 0.05) μg/L,P ＞0.05] ; the changes of serum ALT and AST:no significant increase after 15min of reperfusion,reach the top at 6h and the level was still higher than normal at 3 d (P ＜ 0.05) ; L-FABP in liver tissue:the expression was decreased after 15min of reperfusion (0.148 ± 0.047,P ＜ 0.05),reached to the trough at 3 h (0.071 ± 0.019,P ＜ 0.05) and returned to normal at 3 d (0.142 ± 0.047,P ＞ 0.05).Compared with group B,the level of serum L-FABP,AST and ALT in group C were significandy increased at each time point (P ＜ 0.05),and the expression of L-FABP was significantly decreased (P ＜ 0.05),the pathological changes were significantly worse.Conclusion Compared with the traditional indicator of liver function (ALT,AST),L-FABP is the more sensitive indicator to monitor the hepatic ischemia-reperfusion injury,and it consistents with the changes in the liver tissue pathology.