中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2012年
10期
1944-1946,后插1
,共4页
林福生%沈世强%闫瑞承%陈祖兵%王琦
林福生%瀋世彊%閆瑞承%陳祖兵%王琦
림복생%침세강%염서승%진조병%왕기
雌激素%缺血再灌注损伤%肝切除术%肝移植%细胞周期蛋白D1
雌激素%缺血再灌註損傷%肝切除術%肝移植%細胞週期蛋白D1
자격소%결혈재관주손상%간절제술%간이식%세포주기단백D1
Estrogen%Ischemia-reperfusion injury%Hepatectomy%Liver transplantation%Cyclin D1
目的 观察17-β雌二醇对大鼠肝切除肝缺血再灌注损伤后残肝再生的影响.方法 雄性SD大鼠随机分为单纯50％肝切除组(R组)、50％肝切除肝缺血再灌注损伤组(R+IR组)及17-β雌二醇处理组(E2 +R+IR组).观察术后7d生存率、血清谷丙转氨酶(ALT)、肝脏组织学及肝脏再生情况.结果 与R +IR组比较,17-β雌二醇显著延长大鼠生存率(80％比20％,P＜0.05),各时间点ALT水平显著下降(P＜0.05),病理损伤明显改善,再生肝重量(RLW)明显升高,在再灌注后2d残肝组织细胞核增殖抗原(Ki-67)、增殖细胞核抗原(PCNA)和细胞周期蛋白D1(Cyclin D1)表达显著增多.结论 7-β雌二醇对大鼠肝切除肝缺血再灌注损伤后残肝的再生具有促进作用,其作用机制可能与诱导Cyclin D1的高表达有关.
目的 觀察17-β雌二醇對大鼠肝切除肝缺血再灌註損傷後殘肝再生的影響.方法 雄性SD大鼠隨機分為單純50％肝切除組(R組)、50％肝切除肝缺血再灌註損傷組(R+IR組)及17-β雌二醇處理組(E2 +R+IR組).觀察術後7d生存率、血清穀丙轉氨酶(ALT)、肝髒組織學及肝髒再生情況.結果 與R +IR組比較,17-β雌二醇顯著延長大鼠生存率(80％比20％,P＜0.05),各時間點ALT水平顯著下降(P＜0.05),病理損傷明顯改善,再生肝重量(RLW)明顯升高,在再灌註後2d殘肝組織細胞覈增殖抗原(Ki-67)、增殖細胞覈抗原(PCNA)和細胞週期蛋白D1(Cyclin D1)錶達顯著增多.結論 7-β雌二醇對大鼠肝切除肝缺血再灌註損傷後殘肝的再生具有促進作用,其作用機製可能與誘導Cyclin D1的高錶達有關.
목적 관찰17-β자이순대대서간절제간결혈재관주손상후잔간재생적영향.방법 웅성SD대서수궤분위단순50％간절제조(R조)、50％간절제간결혈재관주손상조(R+IR조)급17-β자이순처리조(E2 +R+IR조).관찰술후7d생존솔、혈청곡병전안매(ALT)、간장조직학급간장재생정황.결과 여R +IR조비교,17-β자이순현저연장대서생존솔(80％비20％,P＜0.05),각시간점ALT수평현저하강(P＜0.05),병리손상명현개선,재생간중량(RLW)명현승고,재재관주후2d잔간조직세포핵증식항원(Ki-67)、증식세포핵항원(PCNA)화세포주기단백D1(Cyclin D1)표체현저증다.결론 7-β자이순대대서간절제간결혈재관주손상후잔간적재생구유촉진작용,기작용궤제가능여유도Cyclin D1적고표체유관.
Objective To investigate whether 17-βestradiol can promote regeneration of the remnant liver after semi-hepatectomy with 45 minutes of hepatic ischemia in rats.Methods Male Sprague-Dawley rats were randomly allocated into 3 groups:R group,rats subjected to 50％ hepatectomy alone;R + IR group,rats subjected to 50％ hepatectomy and 45min of hepatic ischemia; E2 + R + IR group,rats administration with 17-βestradiol.The 7d survival rate,serum alanine aminotransferase (ALT) level,liver histology and remnant liver regeneration were then investigated.Results Compared with R + IR group,17-βestradiol significantly improved the 7 d survival rate (80％ vs 20％,P ＜ 0.05),decreased ALT levels (P ＜0.05) and increased the RLW (P ＜0.05).The pathological changes at 12h after reperfusion were milder and the expression of Ki-67,proliferating cell nuclear antigen (PCNA) and Cyclin D1 in the 2d after reperfusion were significantly higher in E2 + R + IR group comparison to those in the R + IR group.Conclusion 17-βestradiol could promote regeneration of the remnant liver after semi-hepatectomy with 45 minutes of hepatic ischemia in rats.The high expression of Cyclin D1 may be one of the reasons.
