中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2012年
10期
1957-1959
,共3页
狄冬梅%张晓膺%罗光华%郑璐%蒋波%Peter Nilsson-Ehle%徐宁
狄鼕梅%張曉膺%囉光華%鄭璐%蔣波%Peter Nilsson-Ehle%徐寧
적동매%장효응%라광화%정로%장파%Peter Nilsson-Ehle%서저
载脂蛋白M%瘦素%载脂蛋白B%低密度脂蛋白受体
載脂蛋白M%瘦素%載脂蛋白B%低密度脂蛋白受體
재지단백M%수소%재지단백B%저밀도지단백수체
Apolipoprotein M%Leptin%Apolipoprotein B%Low density lipoprotein receptor
目的 观察瘦素对正常SD大鼠肝脏载脂蛋白M(ApoM)、载脂蛋白AI (ApoAI)、载脂蛋白B(ApoB)和低密度脂蛋白受体(LDL-R)表达的影响.方法 通过给健康SD大鼠尾静脉置管,微量注射泵持续泵入瘦素的方法建立高瘦素模型,对照组泵入生理盐水.6h后检测大鼠肝脏ApoM表达的变化,以及血清ApoM、脂蛋白的变化.通过建立离体肝灌注模型,检测肝脏ApoM mRNA的变化.结果 在尾静脉实验中,瘦素组较生理盐水组血糖增加了14.68％ (P＜0.01),胰岛素水平下降了68.82％(P＜0.05),大鼠肝脏ApoM、ApoB和LDL-R mRNA水平分别下降了55.15％ (P ＜0.05)、48.60％(P＜0.01)及40.43％ (P ＜0.05),而ApoAI表达增加了149.00％ (P ＜0.01).但在离体肝灌注模型中,瘦素对ApoM、ApoAI、ApoB和LDL-R的表达无明显影响(P＞0.05).结论 瘦素对大鼠肝脏脂蛋白和LDL-R表达的影响可能通过其他信使介导,具体机制还需进一步研究.
目的 觀察瘦素對正常SD大鼠肝髒載脂蛋白M(ApoM)、載脂蛋白AI (ApoAI)、載脂蛋白B(ApoB)和低密度脂蛋白受體(LDL-R)錶達的影響.方法 通過給健康SD大鼠尾靜脈置管,微量註射泵持續泵入瘦素的方法建立高瘦素模型,對照組泵入生理鹽水.6h後檢測大鼠肝髒ApoM錶達的變化,以及血清ApoM、脂蛋白的變化.通過建立離體肝灌註模型,檢測肝髒ApoM mRNA的變化.結果 在尾靜脈實驗中,瘦素組較生理鹽水組血糖增加瞭14.68％ (P＜0.01),胰島素水平下降瞭68.82％(P＜0.05),大鼠肝髒ApoM、ApoB和LDL-R mRNA水平分彆下降瞭55.15％ (P ＜0.05)、48.60％(P＜0.01)及40.43％ (P ＜0.05),而ApoAI錶達增加瞭149.00％ (P ＜0.01).但在離體肝灌註模型中,瘦素對ApoM、ApoAI、ApoB和LDL-R的錶達無明顯影響(P＞0.05).結論 瘦素對大鼠肝髒脂蛋白和LDL-R錶達的影響可能通過其他信使介導,具體機製還需進一步研究.
목적 관찰수소대정상SD대서간장재지단백M(ApoM)、재지단백AI (ApoAI)、재지단백B(ApoB)화저밀도지단백수체(LDL-R)표체적영향.방법 통과급건강SD대서미정맥치관,미량주사빙지속빙입수소적방법건립고수소모형,대조조빙입생리염수.6h후검측대서간장ApoM표체적변화,이급혈청ApoM、지단백적변화.통과건립리체간관주모형,검측간장ApoM mRNA적변화.결과 재미정맥실험중,수소조교생리염수조혈당증가료14.68％ (P＜0.01),이도소수평하강료68.82％(P＜0.05),대서간장ApoM、ApoB화LDL-R mRNA수평분별하강료55.15％ (P ＜0.05)、48.60％(P＜0.01)급40.43％ (P ＜0.05),이ApoAI표체증가료149.00％ (P ＜0.01).단재리체간관주모형중,수소대ApoM、ApoAI、ApoB화LDL-R적표체무명현영향(P＞0.05).결론 수소대대서간장지단백화LDL-R표체적영향가능통과기타신사개도,구체궤제환수진일보연구.
Objective To observe the effects of leptin on the expression of apolipoprotein M' s (ApoM) expression in HepG2 cells.Methods A series experiments were performed in the isolated rat's liver perfusion and in normal rats.ApoM protein concentrations in serum were determined by the dot-blotting method and ApoM mRNA levels in liver tissues were determined by real-time reverse transcription-polymerase chain reaction (RT-PCR).Results After short time leptin administration,serum insulin levels were significantly decreased by 68.82％ (P ＜ 0.05),at the meanwhile blood glucose levels were increased by 14.68％ (P ＜ 0.01) in the leptin treated rats compared to the control rats.Moreover,leptin could significantly decrease hepatic mRNA levels of ApoM,ApoB and LDL-R by 55.15％ (P ＜ 0.05),48.60％ (P ＜0.01) and 40.43％ (P ＜ 0.05),respectively.whereas ApoAI mRNA level was increased by 149.00％(P ＜0.01) in the leptin treated rats.However,Leptin could not alter expression of ApoM,ApoAI,ApoB and LDL-R in the isolated liver perfusion (P ＞ O.05).Conclusion Leptin influences hepatic expressions of apolipoproteins and LDL-R needs secondary mediator in rats' body.The detailed mechanism needs further investigation.
