中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2012年
10期
1966-1969
,共4页
张文筱%方翼%戴淑琴%吴仲凯%赵擎宇
張文篠%方翼%戴淑琴%吳仲凱%趙擎宇
장문소%방익%대숙금%오중개%조경우
脓毒症%多烯磷脂酰胆碱%过氧化物酶体增殖物激活受体γ%血流动力学
膿毒癥%多烯燐脂酰膽堿%過氧化物酶體增殖物激活受體γ%血流動力學
농독증%다희린지선담감%과양화물매체증식물격활수체γ%혈류동역학
Sepsis%Polyenylphosphatidylcholine%Peroxisome proliferator activated receptor γ%Hemodynamic
目的 探讨多烯磷脂酰胆碱(PPC)对大鼠脓毒症模型的保护作用及其机制.方法 利用大鼠盲肠结扎穿孔(CLP)模型建立大鼠脓毒症模型.将75只SD雄性大鼠随机分为假手术(Sham)组、PPC对照(Sham-PPC)组、脓毒症(CLP)组、PPC保护(CLP-PPC)组、过氧化物酶体增殖物激活受体γ(PPARγ)拮抗(CLP-PPC/GW9662)组.另外两组大鼠观察CLP组与CLP-PPC组脓毒症建立后7d生存率.模型建立18h后测量血流动力学指标、血糖(GLU)、动脉血气、谷丙转氨酶(ALT)、谷草转氨酶(AST)、一氧化氮(NO)、超氧化物歧化酶(SOD)、丙二醛(MDA)、肿瘤坏死因子(TNF)-α水平及肝脏湿/干重比(W/D).结果 脓毒症建立7d后,CLP组仅有4(4/20)只大鼠存活,而CLP-PPC组有12(12/20)只大鼠存活.PPC能够纠正脓毒症休克状态,显著改善血流动力学指标(P＜0.01).CLP-PPC组肝脏炎症损伤较CLP组轻,W/D、ALT、AST、LAC、TNF-α、NO和MDA 水平显著降低,SOD活力及血糖显著升高,差异有统计学意义(P＜0.05).静脉注射PPC前给予GW9662,能够阻断PPC对脓毒症大鼠的保护作用.结论 预保护应用PPC能够显著降低脓毒症大鼠肝脏炎症反应,改善肝代谢功能,纠正感染性休克.PPC对脓毒症大鼠的抗炎保护作用与PPARγ有关.
目的 探討多烯燐脂酰膽堿(PPC)對大鼠膿毒癥模型的保護作用及其機製.方法 利用大鼠盲腸結扎穿孔(CLP)模型建立大鼠膿毒癥模型.將75隻SD雄性大鼠隨機分為假手術(Sham)組、PPC對照(Sham-PPC)組、膿毒癥(CLP)組、PPC保護(CLP-PPC)組、過氧化物酶體增殖物激活受體γ(PPARγ)拮抗(CLP-PPC/GW9662)組.另外兩組大鼠觀察CLP組與CLP-PPC組膿毒癥建立後7d生存率.模型建立18h後測量血流動力學指標、血糖(GLU)、動脈血氣、穀丙轉氨酶(ALT)、穀草轉氨酶(AST)、一氧化氮(NO)、超氧化物歧化酶(SOD)、丙二醛(MDA)、腫瘤壞死因子(TNF)-α水平及肝髒濕/榦重比(W/D).結果 膿毒癥建立7d後,CLP組僅有4(4/20)隻大鼠存活,而CLP-PPC組有12(12/20)隻大鼠存活.PPC能夠糾正膿毒癥休剋狀態,顯著改善血流動力學指標(P＜0.01).CLP-PPC組肝髒炎癥損傷較CLP組輕,W/D、ALT、AST、LAC、TNF-α、NO和MDA 水平顯著降低,SOD活力及血糖顯著升高,差異有統計學意義(P＜0.05).靜脈註射PPC前給予GW9662,能夠阻斷PPC對膿毒癥大鼠的保護作用.結論 預保護應用PPC能夠顯著降低膿毒癥大鼠肝髒炎癥反應,改善肝代謝功能,糾正感染性休剋.PPC對膿毒癥大鼠的抗炎保護作用與PPARγ有關.
목적 탐토다희린지선담감(PPC)대대서농독증모형적보호작용급기궤제.방법 이용대서맹장결찰천공(CLP)모형건립대서농독증모형.장75지SD웅성대서수궤분위가수술(Sham)조、PPC대조(Sham-PPC)조、농독증(CLP)조、PPC보호(CLP-PPC)조、과양화물매체증식물격활수체γ(PPARγ)길항(CLP-PPC/GW9662)조.령외량조대서관찰CLP조여CLP-PPC조농독증건립후7d생존솔.모형건립18h후측량혈류동역학지표、혈당(GLU)、동맥혈기、곡병전안매(ALT)、곡초전안매(AST)、일양화담(NO)、초양화물기화매(SOD)、병이철(MDA)、종류배사인자(TNF)-α수평급간장습/간중비(W/D).결과 농독증건립7d후,CLP조부유4(4/20)지대서존활,이CLP-PPC조유12(12/20)지대서존활.PPC능구규정농독증휴극상태,현저개선혈류동역학지표(P＜0.01).CLP-PPC조간장염증손상교CLP조경,W/D、ALT、AST、LAC、TNF-α、NO화MDA 수평현저강저,SOD활력급혈당현저승고,차이유통계학의의(P＜0.05).정맥주사PPC전급여GW9662,능구조단PPC대농독증대서적보호작용.결론 예보호응용PPC능구현저강저농독증대서간장염증반응,개선간대사공능,규정감염성휴극.PPC대농독증대서적항염보호작용여PPARγ유관.
Objective To investigate the protective effects of polyenylphosphatidylcholine (PPC) in rat cecal ligation and puncture (CLP) model and the underlying mechanisms.Methods Prospective,randomized and controlled animal study was performed.Seventy-five male Sprague-Dawley rats were randomly divided into five groups by using random number table:sham group,sham-PPC group,CLP group,CLP-PPC group,and CLP-PPC/GW9662 group in which peroxisome proliferator activated receptor γ (PPARγ) antagonist GW9662 was administrated before the pretreatment of PPC in CLP rats.The 7-day survival of CLP group and CLP-PPC group was monitored after the introduction of sepsis.The rats were subjected to sepsis by CLP.The hemodynamic parameters of all animals were recorded 18 h after the surgery and the samples were collected.The arterial blood gas was analyzed,and the levels of liver wet/dry ratio (W/D),aspartate aminotransferase (AST),alanine aminotransferase (ALT),nitric oxide (NO),superoxidase dismutase (SOD),malondialdehyde (MDA) and tumor necrosis factor (TNF)-α in serum were determined.Results Seven days after CLP,there were only 4 (4/20) rats left in CLP group while 12 (12/20) rats survived in CLP-PPC group.PPC could reverse the hypodynamic collapse in sepsis (P ＜0.01).As compared with CLP group,the rats in CLP-PPC group showed less severe hepatic inflammation.The levels of W/D,ALT,AST,TNF-α,NO,MDA and LAC were significantly lower in CLP-PPC group while blood glucose and the activity of SOD were significantly increased(P ＜ 0.05).GW9662 blocked the protective effects of PPC in sepsis.Conclusion Intravenous administration of PPC before the onset of sepsis may alleviate hepatic injury in sepsis,improve liver functions and reverse septic shock.PPC exerts its protective influence on rat sepsis model partially through PPARγ.
