CAJ | 학술논문

目的观察低相对分子质量肝素(LMWH)对裸鼠胃癌模型CD40/CD40L表达的影响.方法应用原位移植方法建立裸鼠胃癌原位模型,术后7d将裸鼠模型随机分为2组:对照组、LMWH治疗组.每周注射药物2次,共5周,第6周处死裸鼠,观察两组裸鼠胃癌模型成瘤情况,采用免疫组织化学染色法检测肿瘤组织CD40/CD40L的表达.结果 LWMH组胃癌原位模型肿瘤体积较对照组明显缩小,抑瘤率为42.25％,远处转移率为20％,较对照组70％显著降低(P＜0.05)；LMWH组CD40表达水平显著低于对照组(P＜0.05),而CD40L表达水平显著高于对照组(P＜0.05).结论 LMWH可抑制裸鼠胃癌的增殖及转移,其机制可能与干预肿瘤CD40/CD40L通路有关.
목적 관찰저상대분자질량간소(LMWH)대라서위암모형CD40/CD40L표체적영향.방법 응용원위이식방법건립라서위암원위모형,술후7d장라서모형수궤분위2조:대조조、LMWH치료조.매주주사약물2차,공5주,제6주처사라서,관찰량조라서위암모형성류정황,채용면역조직화학염색법검측종류조직CD40/CD40L적표체.결과 LWMH조위암원위모형종류체적교대조조명현축소,억류솔위42.25％,원처전이솔위20％,교대조조70％현저강저(P＜0.05)；LMWH조CD40표체수평현저저우대조조(P＜0.05),이CD40L표체수평현저고우대조조(P＜0.05).결론 LMWH가억제라서위암적증식급전이,기궤제가능여간예종류CD40/CD40L통로유관.
Objective To study the effect of Low-molecular weight heparins on the expression of CD40/CD40L in orthotopic transplantation gastric tumor model in nude mice.Methods A model of human gastric cancer was established by orthotopic transplantation of histologically intact tumor tissue blocks in the gastric walls of nude mice.All mice were randomly divided into 2 groups:control group and LMWH group.Forty-two days after treatment,tumor proliferation and metastasis rates were evaluated respectively,The expressions of CD40/CD40L proteins were detected by immunohistochemistry.Results In comparison with that in the control group,the tumor volume of LMWH group was reduced significantly,and the inhibitory rate was 42.25％.The distant metastasis rate (20％) compared with the control group (70％) was significantly lower (P ＜ 0.05) ;The expressions of CD40 protein in the LMWH group was significantly lower than that in the control group (P ＜0.05),The expressions of CD40L protein in the LMWH group was significantly higher (P ＜ 0.05).Conclusion Low-molecular weight heparins has inhibitory effects on tumor proliferation and metastasis,the mechanism may interfere in CD40/CD40L passageway.