CAJ | 학술논문

目的观察犬梗阻小肠黏膜紧密连接的变化和Claudin2的表达,探讨手术松解时机对紧密连接修复的影响.方法 40条犬制作低位小肠梗阻模型,随机分为对照组、肠梗阻72、120 h组和以上2时相点的手术松解组,透射电镜观察各组小肠黏膜紧密连接变化,免疫组织化学方法观察黏膜Claudin2表达.结果肠梗阻72 h梗阻近段黏膜紧密连接缩短、模糊,Claudin2相对吸光度值(32.83),较对照组(94.84)表达下调(P＜0.01),松解梗阻后紧密连接修复,Claudin2 (46.67)较梗阻72 h组表达上调(P＜0.01).肠梗阻120 h紧密连接消失,松解梗阻后未见修复,Claudin2(7.33 ～9.63)较梗阻120 h组(6.37 ～7.83)表达未见上调.结论肠梗阻致黏膜紧密连接破坏的严重程度与肠梗阻时间相关,肠梗阻120 h后肠黏膜紧密连接的损伤不可修复,肠梗阻的早期诊断和积极治疗非常重要.
목적 관찰견경조소장점막긴밀련접적변화화Claudin2적표체,탐토수술송해시궤대긴밀련접수복적영향.방법 40조견제작저위소장경조모형,수궤분위대조조、장경조72、120 h조화이상2시상점적수술송해조,투사전경관찰각조소장점막긴밀련접변화,면역조직화학방법관찰점막Claudin2표체.결과 장경조72 h경조근단점막긴밀련접축단、모호,Claudin2상대흡광도치(32.83),교대조조(94.84)표체하조(P＜0.01),송해경조후긴밀련접수복,Claudin2 (46.67)교경조72 h조표체상조(P＜0.01).장경조120 h긴밀련접소실,송해경조후미견수복,Claudin2(7.33 ～9.63)교경조120 h조(6.37 ～7.83)표체미견상조.결론 장경조치점막긴밀련접파배적엄중정도여장경조시간상관,장경조120 h후장점막긴밀련접적손상불가수복,장경조적조기진단화적겁치료비상중요.
Objective To observe tight junction (TJ) of intestinal epithelial and expression of Claudin2 after small bowel obstruction in dogs,and investigate the effect of surgical adhesiolysis opportunity on the repair of TJ.Methods Forty dogs were divided into 72-h obstruction group,120-h obstruction group,72-h adhesiolysis group,120-adhesiolysis group and control group.Models of mechanical bowel obstruction at the ileum were made in each test group.At definite time point samples of intestinal mueosa were obtained and examined under the transmission electron microscopy.The expression of claudin2 in the intestinal mucosa was detected by using immunohistochemistry.Results At 72nd h after bowel obstruction,it was found TJ was shortened and dim,and the expression of claudin2 (relative absorbance =32.83) was down-regulated as compared with 94.84 in control group (P ＜ 0.01).At 120 h after adhesiolysis,TJ was repaired,and the expression of claudin 2 (46.67) was up-regulated as compared with 72-h obstruction group.At 120 h after obstruction,TJ disappeared,and no repair was found after adhesiolysis.The expression of claudin2 (6.37-7.83) was also down-regulated obviously.In 120-h adhesiolysis group,there was no repair in TJ,and there was slight up-regulated expression of claudin2 (7.33-9.67) as compared with 120-h obstruction group.Conclusion Bowl obstruction damaged TJ of intestinal mucosa,and resulted in down-regulated expression of claudin2.The severity of the injury was related with the duration of obstruction.120 h after bowel obstruction,TJ injury can' t be repaired by surgical adhesiolysis.