CAJ | 학술논문

目的探讨结直肠癌组织中丝氨酸257/苏氨酸261位点磷酸化丝裂原活化蛋白激酶激酶4 (pMKK4)表达与结直肠癌临床病理的相关性.方法通过构建组织芯片,用免疫组织化学染色方法检测并比较96例含不同分期的散发结直肠癌、24例结直肠腺瘤及24例正常结直肠黏膜组织中pMKK4的表达,结合临床病理资料进行分析.结果 pMKK4在正常结直肠黏膜中高水平表达,在腺瘤和结直肠癌组织中表达水平有不同程度的下降(P＜0.01).pMKK4表达水平与浸润深度、远处转移及肝转移相关(P＜0.05),与性别、年龄、分化程度、KRAS突变、淋巴结转移及TNM分期无明显相关(P＞0.05).结论丝氨酸257/苏氨酸261位点pMKK4可能与结直肠癌的发生发展有关.其表达水平在结直肠癌组织中降低,且与远处转移相关.
목적 탐토결직장암조직중사안산257/소안산261위점린산화사렬원활화단백격매격매4 (pMKK4)표체여결직장암림상병리적상관성.방법 통과구건조직심편,용면역조직화학염색방법검측병비교96례함불동분기적산발결직장암、24례결직장선류급24례정상결직장점막조직중pMKK4적표체,결합림상병리자료진행분석.결과 pMKK4재정상결직장점막중고수평표체,재선류화결직장암조직중표체수평유불동정도적하강(P＜0.01).pMKK4표체수평여침윤심도、원처전이급간전이상관(P＜0.05),여성별、년령、분화정도、KRAS돌변、림파결전이급TNM분기무명현상관(P＞0.05).결론 사안산257/소안산261위점pMKK4가능여결직장암적발생발전유관.기표체수평재결직장암조직중강저,차여원처전이상관.
Objective To investigate the expression of phosphorylated mitogen-activated protein kinase kinase 4 (pMKK4) expression in colorect.al cancer and its association with clinicopathological factors.Methods Specimens and clinicopathological data of 96 cases of colorectal cancer with different stages,24 cases of colorectal adenomas,and 24 cases of normal colonic mucosae were collected.The expression of pMKK4 (S257/T261) was detected by using immunohistochemistry,and its association with clinicopathological factors was analyzed.Results There was strong pMKK4 staining in most normal colonic mucosae.In adenomas and colorectal tumors,pMKK4 expression was reduced (P ＜ 0.01).pMKK4 expression was associated with invasion depth (P ＜ 0.05),distant metastasis (P ＜ 0.05).Conclusion pMKK4 (S257/T261) plays an important role in tumor progression in colorectal cancer,and lowered expression of pMKK4 is associated with metastasis.