中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2012年
10期
2085-2086
,共2页
何玮%李路%余虓%兰儒竹%王少刚%李家贵%刘继红%叶章群
何瑋%李路%餘虓%蘭儒竹%王少剛%李傢貴%劉繼紅%葉章群
하위%리로%여효%란유죽%왕소강%리가귀%류계홍%협장군
细菌性炎症%下尿路%Beagle犬%模型,动物
細菌性炎癥%下尿路%Beagle犬%模型,動物
세균성염증%하뇨로%Beagle견%모형,동물
Bacterial inflammation%Lower urinary tract%The Beagle dog%Model,animal
目的 建立一种简单、稳定、符合临床模式的细菌性下尿路炎Beagle犬动物模型.方法 选用24个月成年Beagle犬6条,随机分为模型组(3条)和对照组(3条),每次模型组采用标准致病菌大肠杆菌(ATCC25922)2.5 ml,对照组采用相同体积的灭菌生理盐水膀胱灌注.模型成功后进行细菌培养鉴定、药敏实验和病理学观察.结果 与对照组比较,模型组的细菌培养差异有统计学意义(P＜0.05),细菌鉴定为同型大肠杆菌,对氨苄西林的耐药率最高,对美罗培南最敏感.病理切片中组织形成改变明显,镜下可见大量炎性细胞浸润.结论 大肠杆菌能导致Beagle犬下尿路炎,并与人类的下尿路炎具有相似性,多次膀胱灌注是建立动物模型简洁、有效的试验方法,并证实细菌的耐药性是导致下尿路感染的主要病因之一.
目的 建立一種簡單、穩定、符閤臨床模式的細菌性下尿路炎Beagle犬動物模型.方法 選用24箇月成年Beagle犬6條,隨機分為模型組(3條)和對照組(3條),每次模型組採用標準緻病菌大腸桿菌(ATCC25922)2.5 ml,對照組採用相同體積的滅菌生理鹽水膀胱灌註.模型成功後進行細菌培養鑒定、藥敏實驗和病理學觀察.結果 與對照組比較,模型組的細菌培養差異有統計學意義(P＜0.05),細菌鑒定為同型大腸桿菌,對氨芐西林的耐藥率最高,對美囉培南最敏感.病理切片中組織形成改變明顯,鏡下可見大量炎性細胞浸潤.結論 大腸桿菌能導緻Beagle犬下尿路炎,併與人類的下尿路炎具有相似性,多次膀胱灌註是建立動物模型簡潔、有效的試驗方法,併證實細菌的耐藥性是導緻下尿路感染的主要病因之一.
목적 건립일충간단、은정、부합림상모식적세균성하뇨로염Beagle견동물모형.방법 선용24개월성년Beagle견6조,수궤분위모형조(3조)화대조조(3조),매차모형조채용표준치병균대장간균(ATCC25922)2.5 ml,대조조채용상동체적적멸균생리염수방광관주.모형성공후진행세균배양감정、약민실험화병이학관찰.결과 여대조조비교,모형조적세균배양차이유통계학의의(P＜0.05),세균감정위동형대장간균,대안변서림적내약솔최고,대미라배남최민감.병리절편중조직형성개변명현,경하가견대량염성세포침윤.결론 대장간균능도치Beagle견하뇨로염,병여인류적하뇨로염구유상사성,다차방광관주시건립동물모형간길、유효적시험방법,병증실세균적내약성시도치하뇨로감염적주요병인지일.
Objective To establish a clinical animal model of bacterial inflammation in the lower urinary tract and to provide a research platform for the physical treatment of lower urinary tract inflammation.Methods Six 24-month-old adult Beagle dogs were randomly divided into model group (n =3) and control group (n =3).By bladder irrigation,the dogs were given the standard pathogenic E.coli (ATCC25922) 2.5 mL in the model group and the same dose of saline in the control group every time.Bacteria were cultured and identified cation,subjected to susceptibility testing and observed pathologically after the model was constructed.Results Compared with the control group,the bacterial culture in the model group was positive and the bacteria identified was the same type of E.coli,the resistance rate of E.coli to ampicillin was the highest and they to meropenem was the most sensitive.HE dyeing show that the changes of tissues were significantly and a great number of inflammatory cells infiltrated under microscope.Conclusion E.coli can cause inflammation in Beagle dogs' lower urinary tract,and the clinical and tissue changes were similarity with the human lower urinary tract inflammation.it is very effective that the multiple bladder irrigation can establish animal model,and the antimicrobial resistance of bacteriais a major cause of lower urinary tract infection.
