中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2012年
11期
2117-2119
,共3页
黄尧%曾永毅%刘景丰%林科灿%罗顺峰%张翔
黃堯%曾永毅%劉景豐%林科燦%囉順峰%張翔
황요%증영의%류경봉%림과찬%라순봉%장상
RNA干扰%癌胚抗原相关细胞黏附分子-6%肝门部胆管癌
RNA榦擾%癌胚抗原相關細胞黏附分子-6%肝門部膽管癌
RNA간우%암배항원상관세포점부분자-6%간문부담관암
RNA interference%Carcinoembryonic antigen-related cell adhesion molecule 6%Hilar cholangiocarcinoma
目的 观察癌胚抗原相关细胞黏附分子-6(CEACAM6)在人肝门部胆管癌细胞株QBC939中的表达,探讨其在侵袭转移中的作用.方法 实时定量聚合酶链反应(Real-time PCR)检测CEACAM6在QBC939细胞中的表达；构建CEACAM6的RNA干扰(RNAi)慢病毒载体,并感染QBC939细胞,Real-time PCR验证RNAi效果；Transwell侵袭试验检测QBC939细胞体外侵袭力的改变.结果 CEACAM6在QBC939细胞中高表达.成功构建CEACAM6基因RNAi慢病毒载体并转染QBC939细胞.和对照组比较,RNAi组CEACAM6 mRNA表达抑制率为93.1％ (P ＜0.05)；Tran-swell实验提示RNAi后,侵袭细胞OD570 RNAi组(0.09 ±0.01)明显少于对照组(0.13 ±0.02),侵袭抑制率为69.2％ (P ＜0.05).结论 CEACAM6在QBC939细胞中高表达,其表达程度与肝门部胆管癌细胞侵袭转移能力呈正相关.
目的 觀察癌胚抗原相關細胞黏附分子-6(CEACAM6)在人肝門部膽管癌細胞株QBC939中的錶達,探討其在侵襲轉移中的作用.方法 實時定量聚閤酶鏈反應(Real-time PCR)檢測CEACAM6在QBC939細胞中的錶達；構建CEACAM6的RNA榦擾(RNAi)慢病毒載體,併感染QBC939細胞,Real-time PCR驗證RNAi效果；Transwell侵襲試驗檢測QBC939細胞體外侵襲力的改變.結果 CEACAM6在QBC939細胞中高錶達.成功構建CEACAM6基因RNAi慢病毒載體併轉染QBC939細胞.和對照組比較,RNAi組CEACAM6 mRNA錶達抑製率為93.1％ (P ＜0.05)；Tran-swell實驗提示RNAi後,侵襲細胞OD570 RNAi組(0.09 ±0.01)明顯少于對照組(0.13 ±0.02),侵襲抑製率為69.2％ (P ＜0.05).結論 CEACAM6在QBC939細胞中高錶達,其錶達程度與肝門部膽管癌細胞侵襲轉移能力呈正相關.
목적 관찰암배항원상관세포점부분자-6(CEACAM6)재인간문부담관암세포주QBC939중적표체,탐토기재침습전이중적작용.방법 실시정량취합매련반응(Real-time PCR)검측CEACAM6재QBC939세포중적표체；구건CEACAM6적RNA간우(RNAi)만병독재체,병감염QBC939세포,Real-time PCR험증RNAi효과；Transwell침습시험검측QBC939세포체외침습력적개변.결과 CEACAM6재QBC939세포중고표체.성공구건CEACAM6기인RNAi만병독재체병전염QBC939세포.화대조조비교,RNAi조CEACAM6 mRNA표체억제솔위93.1％ (P ＜0.05)；Tran-swell실험제시RNAi후,침습세포OD570 RNAi조(0.09 ±0.01)명현소우대조조(0.13 ±0.02),침습억제솔위69.2％ (P ＜0.05).결론 CEACAM6재QBC939세포중고표체,기표체정도여간문부담관암세포침습전이능력정정상관.
Objective To explore the expression of carcinoembryonic antigen-related cell adhesion molecule 6 (CEACAM6) in human hilar cholangiocarcinoma cell line QBC939 and its roles in invasion and metastasis.Methods Real-time polymerase chain reaction (real-time PCR) was used to detect the expression of CEACAM6 in QBC939 cells.Lentiviral-mediated CEACAM6 small interfering RNA (siRNA) was established to infect QBC939 cells.The silencing effect was assayed by real-time PCR.The invasive activity of infected QBC939 cells was analyzed by Transwell invasion assay.Results There was high expression of CEACAM6 in QBC939 cells.Recombinant lentiviral vector expressing siRNA targeting CEACAM6 gene was established successfully and confirmed by PCR and DNA sequencing.The expression of CEACAM6 mRNA in QBC939 cells infected with LV-CEACAM6-siRNA was decreased by 93.1％ as compared with control group (P ＜ 0.05).After CEACAM6 gene was knocked down,Transwell test revealed that the invasion potency of QBC939 cells was significantly suppressed by 69.2％ as compared with control group (P ＜ 0.05).Conclusion There was high expression of CEACAM6 in QBC939 cells.There is a positive correlation between the expression level of CEACAM6 and the invasion potency of hilar cholangiocarcinoma.
