CAJ | 학술논문

目的通过在人皮肤成纤维细胞(HSFs)中过表达TRAP-1-Like Protein(TLP),观察其对细胞生物学性状的影响.方法通过慢病毒途径在人正常皮肤成纤维细胞中获得稳定过表达TLP基因后,检测细胞增殖、收缩活性以及TLP、Ⅰ、Ⅲ型胶原的表达变化.结果慢病毒介导外源性TLP基因在细胞中稳定表达.TLP可协同转化生长因子-β1(TGF-β1)促进细胞活力升高60％(48 h)(P ＜0.05)；纤维凝胶体积变化差异有统计学意义(P＜0.05)；实时定量聚合酶链反应(Real-time PCR)和Western blot结果都显示Ⅰ/Ⅲ型胶原的合成与TLP的表达量呈正相关(P＜0.05),细胞因子TGF-β1刺激下此反应更明显.结论 TLP可促进人成纤维细胞增殖和收缩,并促进其合成Ⅰ/Ⅲ型胶原.
목적 통과재인피부성섬유세포(HSFs)중과표체TRAP-1-Like Protein(TLP),관찰기대세포생물학성상적영향.방법 통과만병독도경재인정상피부성섬유세포중획득은정과표체TLP기인후,검측세포증식、수축활성이급TLP、Ⅰ、Ⅲ형효원적표체변화.결과 만병독개도외원성TLP기인재세포중은정표체.TLP가협동전화생장인자-β1(TGF-β1)촉진세포활력승고60％(48 h)(P ＜0.05)；섬유응효체적변화차이유통계학의의(P＜0.05)；실시정량취합매련반응(Real-time PCR)화Western blot결과도현시Ⅰ/Ⅲ형효원적합성여TLP적표체량정정상관(P＜0.05),세포인자TGF-β1자격하차반응경명현.결론 TLP가촉진인성섬유세포증식화수축,병촉진기합성Ⅰ/Ⅲ형효원.
Objective To investigate the biological effects of overexpression of TRAP-1-like protein (TLP) on human dermal fibroblasts.Methods Lentiviral vectors encoding TLP was constructed to transfect fibroblasts derived from normal human skin.After stable transfection,the variation of cell viability,contraction activity and the expression levels of TLP and COL Ⅰ/Ⅲ genes in various groups induced by transforming growth factor-β1 (TGF-β1) after TLP gene treatment were detected.Results Exogenetic TLP gene delivery system mediated by lentivirus vector could be expressed stably in cells.The cell viability of skin fibroblasts was increased by up to 60％ after TLP treatment cooperated with TGF-β1 (P ＜ 0.05).TLP could facilitate the contraction of collagen gel induced by fibroblasts down to 70％ of its previous volume,Real-time PCR and Western blotting revealed that the synthesis of COL Ⅰ/Ⅲ was positively correlated with the expression of TLP.These variation trends became obvious under the stimulation of TGF-β1.Conclusion TLP was able to improve the overall cell viability of skin fibroblasts,as well as,to promote scar contracture.And the synthesis of COL Ⅰ/Ⅲ was positively correlated with the expression of TLP,especially under the stimulation of TGF-β1.