CAJ | 학술논문

目的观察鱼藤素对胃癌细胞化疗敏感性的影响.方法胃癌SGC-7901细胞以1 μmol/L鱼藤素处理24h后,噻唑蓝(MTT)比色法检测对其对氟尿嘧啶处理48 h的敏感性变化；经鱼藤素联合氟尿嘧啶(12.5 mg/L)处理后形态学观察进一步验证MTT结果；提取1μmol/L鱼藤素处理24 h后细胞总蛋白,Western blot法检测相关蛋白表达水平的变化.结果鱼藤素预处理后可明显增强氟尿嘧啶对SGC-7901细胞的抑制作用,其IC50值降至7.86 mg/L(P＜0.05)；增强氟尿嘧啶对SGC-7901细胞增殖抑制和凋亡诱导作用；鱼藤素可明显降低蛋白激酶B(Akt)的磷酸化水平.结论鱼藤素可通过下调Akt的活性,增强SGC-7901细胞对氟尿嘧啶的敏感性,与化疗药物联用可能有助于提高抗肿瘤治疗效果.
목적 관찰어등소대위암세포화료민감성적영향.방법 위암SGC-7901세포이1 μmol/L어등소처리24h후,새서람(MTT)비색법검측대기대불뇨밀정처리48 h적민감성변화；경어등소연합불뇨밀정(12.5 mg/L)처리후형태학관찰진일보험증MTT결과；제취1μmol/L어등소처리24 h후세포총단백,Western blot법검측상관단백표체수평적변화.결과 어등소예처리후가명현증강불뇨밀정대SGC-7901세포적억제작용,기IC50치강지7.86 mg/L(P＜0.05)；증강불뇨밀정대SGC-7901세포증식억제화조망유도작용；어등소가명현강저단백격매B(Akt)적린산화수평.결론 어등소가통과하조Akt적활성,증강SGC-7901세포대불뇨밀정적민감성,여화료약물련용가능유조우제고항종류치료효과.
Objective To investigate the chemosensitivity of gastric carcinoma cells to deguelin.Methods Human gastric carcinoma cell line SGC-7901 and 5-fluorouracil (5-Fu) were used in present study.The chemosensitivity of cells was determined by the methyl thiazol tetrazolium (MTT) assay after exposure to 1 μmol/L deguelin for 24 h.The results of MTT were further confirmed by morphological analysis of cells treated with 1 mol/L deguelin combined with 5-Fu (12.5 mg/L).Total protein of cells was extracted after treatment with 1 μmol/L deguelin for 24 h,and the expression of proteins related was detected by using Western blotting.Results After pretreatment with deguelin,IC50 of 5-Fu in SGC-7901 cells was reduced to 7.86 mg/L (P ＜ 0.05).Meanwhile,pretreatment with deguelin increased the proliferation inhibition and apoptotic induction of 5-Fu.The phosphorylated Akt was significantly downregulated after treatment with duguelin.Conclusion Deguelin enhanced the sensitivity of SGC-7901 cells to 5-Fu via inhibiting the activation of Akt,and it might be a potential sensitizer to improve the effects of chemotherapy.