中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2012年
11期
2208-2210
,共3页
罗长江%焦作义%李继鹏%王为忠
囉長江%焦作義%李繼鵬%王為忠
라장강%초작의%리계붕%왕위충
胸腺内注射%骨髓细胞%小肠移植%免疫耐受
胸腺內註射%骨髓細胞%小腸移植%免疫耐受
흉선내주사%골수세포%소장이식%면역내수
Injection in thymus%Bone marrow cell%Small intestine transplantation%Immune tolerance
目的 探讨异基因骨髓注射在大鼠小肠移植中的免疫耐受作用和意义.方法 选用近交系大鼠F344/N和Wistar/A进行全小肠异位移植,实验组在异基因移植前7d取供体BMC行受体胸腺内注入,对照单纯同基因及异基因大鼠移植模型了解异基因供体骨髓在受体胸腺内注射能否减少移植后急性排斥反应的发生,观察其生存时间、病理结果及CD4、CD8、CD25的变化.结果 (1)A组移植大鼠平均存活时间为(7.33±1.03) d;B组为(43.76 ±4.57) d;C组为(55.28±7.48)d.(2)异基因大鼠异位全小肠移植术后3、7、15 d可出现典型的轻、中、重度排斥反应,而同基因组和实验组中未出现排斥反应.(3)异基因移植组术后CD4、CD25+,高于其他组(P＜0.05).而CD8的变化差异无统计学意义(P＞0.05).结论 移植术前7d异基因供体骨髓在受体胸腺内注射能显著减少小肠移植后急性排斥反应的发生,并可以抑制移植肠CD4、CD25细胞的表达.
目的 探討異基因骨髓註射在大鼠小腸移植中的免疫耐受作用和意義.方法 選用近交繫大鼠F344/N和Wistar/A進行全小腸異位移植,實驗組在異基因移植前7d取供體BMC行受體胸腺內註入,對照單純同基因及異基因大鼠移植模型瞭解異基因供體骨髓在受體胸腺內註射能否減少移植後急性排斥反應的髮生,觀察其生存時間、病理結果及CD4、CD8、CD25的變化.結果 (1)A組移植大鼠平均存活時間為(7.33±1.03) d;B組為(43.76 ±4.57) d;C組為(55.28±7.48)d.(2)異基因大鼠異位全小腸移植術後3、7、15 d可齣現典型的輕、中、重度排斥反應,而同基因組和實驗組中未齣現排斥反應.(3)異基因移植組術後CD4、CD25+,高于其他組(P＜0.05).而CD8的變化差異無統計學意義(P＞0.05).結論 移植術前7d異基因供體骨髓在受體胸腺內註射能顯著減少小腸移植後急性排斥反應的髮生,併可以抑製移植腸CD4、CD25細胞的錶達.
목적 탐토이기인골수주사재대서소장이식중적면역내수작용화의의.방법 선용근교계대서F344/N화Wistar/A진행전소장이위이식,실험조재이기인이식전7d취공체BMC행수체흉선내주입,대조단순동기인급이기인대서이식모형료해이기인공체골수재수체흉선내주사능부감소이식후급성배척반응적발생,관찰기생존시간、병리결과급CD4、CD8、CD25적변화.결과 (1)A조이식대서평균존활시간위(7.33±1.03) d;B조위(43.76 ±4.57) d;C조위(55.28±7.48)d.(2)이기인대서이위전소장이식술후3、7、15 d가출현전형적경、중、중도배척반응,이동기인조화실험조중미출현배척반응.(3)이기인이식조술후CD4、CD25+,고우기타조(P＜0.05).이CD8적변화차이무통계학의의(P＞0.05).결론 이식술전7d이기인공체골수재수체흉선내주사능현저감소소장이식후급성배척반응적발생,병가이억제이식장CD4、CD25세포적표체.
Objective To investigate the immune tolerance function and significance of allogene bone marrow injection to the rat small intestines transplantation.Methods Selecting inbreeding line rat F344/N and wistar/A to heterotopic graft to the whole small intestine,Before 7 days of allogene transplantation,the testing group get donator BMC and inject it into acceptor thymus.According to the isogene and allogene rat transplant model to comprehend whether injecting allogene donator marrow into acceptor thymus can decrease the acute rejection after transplantation.,To observe the survival time,pathological results and the range of CD4,CD8,CD25.Results (1) The survival time of A group is (7.33 ± 1.03) d,B group is (43.76±4.57) d; C group is(55.28 ±7.48)d. (2) After 3,7 or 14days of allogeneic rat dystopia whole small intestine transplantation,it will appear typical eject reaction,but there is no reject reaction in isogenome and testing group.(3) After transplantation.CD4,CD25 level is higher than other groups (P ＜ 0.05),but it has no clear difference of CD8 level.Conclusion Before 7days of small intestina transplantation,injecting allogenic donator bone marrow into acceptor thymus can reduce the reject reaction after the grafting.And can decreasd the expression of CD4,CD25 cells in small intestine.
