中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2012年
11期
2219-2221
,共3页
闫长青%赵玉沛%刘三光%戚诚%张建生%王文斌
閆長青%趙玉沛%劉三光%慼誠%張建生%王文斌
염장청%조옥패%류삼광%척성%장건생%왕문빈
胰腺癌%血管内皮细胞生长因子%碱性成纤维细胞生长因子
胰腺癌%血管內皮細胞生長因子%堿性成纖維細胞生長因子
이선암%혈관내피세포생장인자%감성성섬유세포생장인자
Pancreatic carcinoma%Vascular endothelial growth factor%Basic fibroblast growth factor
目的 观察碱性成纤维细胞生长因子(bFGF)小干扰RNA(siRNA)对胰腺癌细胞血管内皮生长因子(VEGF)表达水平的影响.方法 利用siRNA技术抑制VEGF及bFGF表达,通过逆转录-聚合酶链反应(RT-PCR)检测及酶联免疫吸附试验(ELISA)检测胰腺癌细胞株VEGF表达变化.结果 Panc-1及PCT-3细胞VEGF mRNA表达受到显著抑制,Panc-1为0.20 ±0.05,PCT-3为0.23±0.02.sw1990及PCT-3分泌VEGF受到显著抑制,sw1990为(1940.67 ±93.84) ng/L,对照组为(2560.67±181.79) ng/L；PCT-3为(174.00±98.73) ng/L,对照组为(779.33 ±317.52) ng/L.结论 bFGFsiRNA能够抑制胰腺癌细胞VEGF核酸及蛋白水平表达.
目的 觀察堿性成纖維細胞生長因子(bFGF)小榦擾RNA(siRNA)對胰腺癌細胞血管內皮生長因子(VEGF)錶達水平的影響.方法 利用siRNA技術抑製VEGF及bFGF錶達,通過逆轉錄-聚閤酶鏈反應(RT-PCR)檢測及酶聯免疫吸附試驗(ELISA)檢測胰腺癌細胞株VEGF錶達變化.結果 Panc-1及PCT-3細胞VEGF mRNA錶達受到顯著抑製,Panc-1為0.20 ±0.05,PCT-3為0.23±0.02.sw1990及PCT-3分泌VEGF受到顯著抑製,sw1990為(1940.67 ±93.84) ng/L,對照組為(2560.67±181.79) ng/L；PCT-3為(174.00±98.73) ng/L,對照組為(779.33 ±317.52) ng/L.結論 bFGFsiRNA能夠抑製胰腺癌細胞VEGF覈痠及蛋白水平錶達.
목적 관찰감성성섬유세포생장인자(bFGF)소간우RNA(siRNA)대이선암세포혈관내피생장인자(VEGF)표체수평적영향.방법 이용siRNA기술억제VEGF급bFGF표체,통과역전록-취합매련반응(RT-PCR)검측급매련면역흡부시험(ELISA)검측이선암세포주VEGF표체변화.결과 Panc-1급PCT-3세포VEGF mRNA표체수도현저억제,Panc-1위0.20 ±0.05,PCT-3위0.23±0.02.sw1990급PCT-3분비VEGF수도현저억제,sw1990위(1940.67 ±93.84) ng/L,대조조위(2560.67±181.79) ng/L；PCT-3위(174.00±98.73) ng/L,대조조위(779.33 ±317.52) ng/L.결론 bFGFsiRNA능구억제이선암세포VEGF핵산급단백수평표체.
Objective To investigate the effects of basic fibroblast growth factor (bFGF) small interfering RNA (siRNA) on the expression of vascular endothelial growth factor (VEGF) in pancreatic carcinoma cells.Methods The expression and secretion of VEGF and bFGF in pancreatic carcinoma cells were inhabited by VEGF siRNA and bFGF siRNA.The expression of VEGF in pancreatic carcinoma cells was detected by using reverse transcription-polymerase chain reaction (RT-PCR) and enzyme linked immunosorbent assay (ELISA).Results Both VEGF siRNA and bFGF siRNA significantly inhabited the expression of VEGF mRNA in Panc-1 and PCT-3 cells.Both VEGF siRNA and bFGF siRNA significantly inhabited the expression of VEGF in sw1990 and PCT-3 cells.Conclusion The bFGFsiRNA inhabited the expression of VEGF mRNA and VEGF in pancreatic carcinoma cells.
