CAJ | 학술논문

目的探讨白花蛇舌草诱导胶质瘤U87细胞株凋亡的作用及机制.方法采用不同浓度的白花蛇舌草含药培养基(0、100、200 ml/L)处理U87细胞48 h,流式细胞仪检测对照组与实验组细胞的凋亡率及线粒体膜电位差异,Western blot法检测两组细胞中抗凋亡基因[B细胞淋巴瘤/白血病-2(bcl-2)]和促凋亡基因(bax)的表达水平改变.结果经100、200 ml/L白花蛇舌草处理的U87细胞凋亡率明显增加,分别为(17.31±1.36)％、(41.23±0.72)％,明显高于对照组的(8.11±1.71)％(P＜0.01),呈显著的剂量依赖性；经JC-1染色法处理后,呈绿色荧光的U87细胞比率随含药培养基浓度升高而增多,分别为(11.90±0.20)％、(34.17±2.79)％,明显高于对照组的(5.53±1.20)％(P＜0.05),提示其线粒体膜电位受药物影响而逐步递减；白花蛇舌草能显著上调促凋亡蛋白bax基因的表达并降低bcl-2/bax比率.结论白花蛇舌草通过线粒体依赖性途径诱导人胶质瘤U87细胞的凋亡.
목적 탐토백화사설초유도효질류U87세포주조망적작용급궤제.방법 채용불동농도적백화사설초함약배양기(0、100、200 ml/L)처리U87세포48 h,류식세포의검측대조조여실험조세포적조망솔급선립체막전위차이,Western blot법검측량조세포중항조망기인[B세포림파류/백혈병-2(bcl-2)]화촉조망기인(bax)적표체수평개변.결과 경100、200 ml/L백화사설초처리적U87세포조망솔명현증가,분별위(17.31±1.36)％、(41.23±0.72)％,명현고우대조조적(8.11±1.71)％(P＜0.01),정현저적제량의뢰성；경JC-1염색법처리후,정록색형광적U87세포비솔수함약배양기농도승고이증다,분별위(11.90±0.20)％、(34.17±2.79)％,명현고우대조조적(5.53±1.20)％(P＜0.05),제시기선립체막전위수약물영향이축보체감；백화사설초능현저상조촉조망단백bax기인적표체병강저bcl-2/bax비솔.결론 백화사설초통과선립체의뢰성도경유도인효질류U87세포적조망.
Objective To investigate the possible mechanism of apoptosis induction by hedyotic diffusa (HD) on human glioma U87 cells.Methods U87 cells were cultured for 24 h in the medium containing HD with different concentrations (0,100,200 ml/L).The apoptosis of U87 cells and collapse of mitochondrial membrane potential were examined by flow cytometry.The protein expression levels of bcl-2 and bax were detected by using immunoblot.Results The apoptosis rate in 100 and 200 ml/L HD groups was (17.31 ± 1.36)％ and (41.23 ± 0.72)％ respectively,significantly higher than that in 0 ml/L HD group (P ＜0.01).The JC-1-green-bright rate of cells treated with 100 and 200 ml/L HD was (11.90 ± 0.20) ％ and (34.17 ± 2.79) ％ respectively,significantly higher than untreated U87 cells (P ＜ 0.05).The protein expression level of bax was increased and bcl-2-to-bax ratio was reduced dose-dependently.Conclusion HD induced apoptosis of U87 cells via mitochondrion pathway.