CAJ | 학술논문

目的设计一种大鼠下肢神经及肌肉功能测定系统,以实现对单组肌肉力量变化的无创动态观测,并评估该系统在生理及病理状态下的应用价值.方法测定系统由固定装置、传动装置、张力换能器、电刺激仪、生物信号采集系统及计算机等组成；评估SD雄性大鼠下肢肌肉收缩强度与电刺激脉冲宽度(0.1、0.2、0.3、0.4、0.5、0.6、0.7 ms)/脉冲强度(8、12、16、20、24、28、32、36、40 V)的量效关系；评估A型肉毒毒素腓肠肌麻痹模型(保妥适,1 U/0.1 ml)及对照组(0.1 ml生理盐水)腓肠肌收缩强度的动态变化.结果腓肠肌收缩强度随脉冲强度(0 ～28 V)或脉冲宽度(0～0.4 ms)的递增呈增强趋势；当脉冲强度超过28 V或脉冲宽度达到0.4 ms以上,腓肠肌收缩强度曲线呈平台样改变[达最大值(28.2±2.1)g]；注射肉毒毒素后靶肌肉收缩强度于第7天降至最低水平[(1.87±0.66)g],然后开始缓慢恢复,第3、7、14、30、45、60、75天均小于对照组,差异有统计学意义(P＜0.05).结论大鼠下肢神经肌肉功能测定系统可以实现对单组肌肉功能的无创量化评估,能够有效反映肉毒毒素生物学效应的动态演变规律.
목적 설계일충대서하지신경급기육공능측정계통,이실현대단조기육역량변화적무창동태관측,병평고해계통재생리급병리상태하적응용개치.방법 측정계통유고정장치、전동장치、장력환능기、전자격의、생물신호채집계통급계산궤등조성；평고SD웅성대서하지기육수축강도여전자격맥충관도(0.1、0.2、0.3、0.4、0.5、0.6、0.7 ms)/맥충강도(8、12、16、20、24、28、32、36、40 V)적량효관계；평고A형육독독소비장기마비모형(보타괄,1 U/0.1 ml)급대조조(0.1 ml생리염수)비장기수축강도적동태변화.결과 비장기수축강도수맥충강도(0 ～28 V)혹맥충관도(0～0.4 ms)적체증정증강추세；당맥충강도초과28 V혹맥충관도체도0.4 ms이상,비장기수축강도곡선정평태양개변[체최대치(28.2±2.1)g]；주사육독독소후파기육수축강도우제7천강지최저수평[(1.87±0.66)g],연후개시완만회복,제3、7、14、30、45、60、75천균소우대조조,차이유통계학의의(P＜0.05).결론 대서하지신경기육공능측정계통가이실현대단조기육공능적무창양화평고,능구유효반영육독독소생물학효응적동태연변규률.
Objective To dynamically observe the changes of muscle strength non-invasively,we design a novel rat lower limbs' nerve and muscle function measuring system.The application of the system was also evaluated under physiological and pathological conditions.Methods The system was composed of fixing device,actuating device,tension transducer,electrical stimulator,biological signal acquisition system and computer.Not only the dose-effect relationship of the pulse duration (0.1,0.2,0.3,0.4,0.5,0.6,0.7 ms) and pulse strength (8,12,16,20,24,28,32,36,40 V) on muscle strength,but also the dynamic change of muscle strength in both botulinum toxin-A group and control group were tested.Results There was an increase in muscle strength with increased pulse strength (under 28 V) or with increased pulse duration (under 0.4 ms).When stimulation with a pulse strength above 28 V or a pulse duration above 0.4 ms,a plateau phase would be detected [(28.2 ± 2.1) g].After injection of botulinum toxin,the strength of target muscle was decreased to the lowest level [(1.87 ± 0.66) g] at day 7,followed by a recovery of muscle strength,but still lower than control group (P ＜ 0.05).Conclusion The rat lower limbs' nerve and muscle function measuring system can evaluate muscle function non-invasively and quantitatively,and can effectively reflect the dynamic evolution of botulinum toxin biological effect.