CAJ | 학술논문

目的探讨人参皂甙Rb1对肠缺血/再灌注致急性肺损伤的保护效应及核因子NF-E2相关因子2(Nrf2)/血红素氧合酶-1(HO-1)通路参与该效应的分子机制.方法成年雄性C57BL/6J小鼠随机分为5组:假手术组(S组)；肠缺血/再灌注组(I/R组)；再灌注+Rb1组(I/R +Rb1组)；全反式维甲酸(ATRA)+再灌注组(ATRA+ I/R组)；ATRA+再灌注+Rb1组(ATRA+ I/R+Rb1组).采用肠缺血/再灌注模型,Western blot检测肺组织Nrf2、HO-1表达变化；酶联免疫吸附试验(ELISA)法检测肺组织肿瘤坏死因子(TNF)-α、白细胞介素(IL)-6、IL-10水平；检测超氧化物歧化酶(SOD)活性及丙二醛(MDA)含量；检测肺湿/干比及肺组织病理损伤评分.结果与S组比较,其他4组Nrf2、HO-1蛋白表达,TNF-α、IL-6、MDA含量,肺组织湿/干重比及肺组织病理评分增高(P＜0.05)；与1/R组比较,1/R+ Rb1组Nrf2,HO-1蛋白表达,TNF-α,IL-6,MDA含量,肺组织湿/干重比及肺组织病理评分降低(P＜0.05)；与I/R+ Rb1组比较,ATRA+ I/R组,ATRA+ I/R+ Rb1组Nrf2、HO-1蛋白表达,NF-α、IL-6、MDA含量,肺组织湿/干重比及肺组织病理评分增高(P＜0.05).SOD活性、IL-10水平与上述变化相反.结论肠缺血/再灌注可引起急性肺损伤,人参皂甙Rb1后处理能通过激活Nrf2/HO-1通路减轻肠缺血/再灌注所致肺损伤.
목적 탐토인삼조대Rb1대장결혈/재관주치급성폐손상적보호효응급핵인자NF-E2상관인자2(Nrf2)/혈홍소양합매-1(HO-1)통로삼여해효응적분자궤제.방법 성년웅성C57BL/6J소서수궤분위5조:가수술조(S조)；장결혈/재관주조(I/R조)；재관주+Rb1조(I/R +Rb1조)；전반식유갑산(ATRA)+재관주조(ATRA+ I/R조)；ATRA+재관주+Rb1조(ATRA+ I/R+Rb1조).채용장결혈/재관주모형,Western blot검측폐조직Nrf2、HO-1표체변화；매련면역흡부시험(ELISA)법검측폐조직종류배사인자(TNF)-α、백세포개소(IL)-6、IL-10수평；검측초양화물기화매(SOD)활성급병이철(MDA)함량；검측폐습/간비급폐조직병리손상평분.결과 여S조비교,기타4조Nrf2、HO-1단백표체,TNF-α、IL-6、MDA함량,폐조직습/간중비급폐조직병리평분증고(P＜0.05)；여1/R조비교,1/R+ Rb1조Nrf2,HO-1단백표체,TNF-α,IL-6,MDA함량,폐조직습/간중비급폐조직병리평분강저(P＜0.05)；여I/R+ Rb1조비교,ATRA+ I/R조,ATRA+ I/R+ Rb1조Nrf2、HO-1단백표체,NF-α、IL-6、MDA함량,폐조직습/간중비급폐조직병리평분증고(P＜0.05).SOD활성、IL-10수평여상술변화상반.결론 장결혈/재관주가인기급성폐손상,인삼조대Rb1후처리능통과격활Nrf2/HO-1통로감경장결혈/재관주소치폐손상.
Objective To investigate the effects of ginsenoside Rb1 on acute lung injury induced by intestinal ischemia reperfusion in mice and role of nuclear factor erythroid 2-related factor 2 (Nrf2)/heme oxygenase-1 (HO-1) pathway in the pathophysiologic process.Methods Male C57BL/6J mice were randomly divided into 5 groups:(1) Sham group (S group) ; (2) Intestinal Ischemia Reperfusion group (I/R group) ; (3) Intestinal Ischemia Reperfusion + 60 mg/kg ginsenoside Rb1 (I/R + Rb 1 group) ; (4) all-trans retinoic acid (ATRA) + I/R group (ATRA + I/R group) ; (5) ATRA + I/R + 60 mg/kg ginsenoside Rb1 (ATRA + I/R + Rb1 group).The Western blotting analysis were performed to observe the expression of Nrf2 and HO-1; tumor necrosis factor-α (TNF-α),interleukin (IL)-6,IL-10,superoxide dismutase (SOD) and malondialdehyde (MDA) were measured;Lung histology was observed and evaluated.Results Intestinal ischemia reperfusion can increase lung injury which was characterized by Nrf2,HO-1,TNF-α,IL-6,MDA,edema (P ＜ 0.05).Rb1 can reduce the Intestinal ischemia reperfusion which decreased by Nrf2,HO-1,TNF-α,IL-6,MDA,edema (P ＜ 0.05).After administration of ATRA,which was an antagonists of Nrf2,Nrf2,HO-1,TNF-α,IL-6,MDA,edema were increased(P ＜ 0.05).SOD was opposite as well as IL-10.Conclusion Ginsenoside Rb1 attenuates acute lung injury induced by intestinal ischemia reperfusion by activating Nrf2/HO-1 pathway.