CAJ | 학술논문

目的探讨枝化状聚乙二醇(PEG)交联去细胞瓣复合材料的皮下抗钙化性能.方法 N-丁二酸-S-乙酰基巯基乙二醇酯(SATA)巯基化修饰去细胞瓣,以相对分子质量为20×103的枝化状丙烯酰化PEG交联获得复合材料(PEG组).以戊二醛固定天然瓣(GA-NAV组)和戊二醛固定去细胞瓣(GA-DAV组)为对照.取3组瓣膜样本各18枚,植入新西兰白兔背部皮下,分别于2、4、8周取出行Von Kossa染色观察钙沉积,原子吸收光谱法测定其钙含量.结果 GA-NAV组钙化发生快且进展迅速,2、4、8周的钙含量依次为(22.39±1.79)、(93.05±6.08)、(174.97±8.34) μg/mg,GA-DAV组钙化程度较轻,钙含量分别为(5.57±2.11)、(31.15 ±5.16)、(72.54±6.50) μg/mg,而PEG组未见明显钙化发生,对应钙含量仅为(0.46±0.07)、(1.68±0.10)、(2.92±0.25) μg/mg,相同时间点组间差异有统计学意义(P＜0.05).结论相对分子质量为20×103的枝化状丙烯酰化PEG交联去细胞瓣复合材料具有良好的抗钙化性能.
목적 탐토지화상취을이순(PEG)교련거세포판복합재료적피하항개화성능.방법 N-정이산-S-을선기구기을이순지(SATA)구기화수식거세포판,이상대분자질량위20×103적지화상병희선화PEG교련획득복합재료(PEG조).이무이철고정천연판(GA-NAV조)화무이철고정거세포판(GA-DAV조)위대조.취3조판막양본각18매,식입신서란백토배부피하,분별우2、4、8주취출행Von Kossa염색관찰개침적,원자흡수광보법측정기개함량.결과 GA-NAV조개화발생쾌차진전신속,2、4、8주적개함량의차위(22.39±1.79)、(93.05±6.08)、(174.97±8.34) μg/mg,GA-DAV조개화정도교경,개함량분별위(5.57±2.11)、(31.15 ±5.16)、(72.54±6.50) μg/mg,이PEG조미견명현개화발생,대응개함량부위(0.46±0.07)、(1.68±0.10)、(2.92±0.25) μg/mg,상동시간점조간차이유통계학의의(P＜0.05).결론 상대분자질량위20×103적지화상병희선화PEG교련거세포판복합재료구유량호적항개화성능.
Objective To assess the anti-calcification ability of branched polyethylene glycol diacrylate (PEG-DA) cross-linked porcine decellularized aortic valves (DAVs) in rabbit subcutaneous model.Methods PEG-DA with a weight of 20 kD was used to cross-link the DAVs which have been modified by SATA.Fresh native aortic valves (NAVs) and DAVs were fixed by 0.625％ glutaraldehyde buffer saline (GA) as control groups.Eighteen samples of each group were implanted subcutaneously in rabbits.Six samples of each group were taken out for calcium staining and quantitative analysis at 2nd,4th and 8th week,respectively.Results Calcium staining showed that severe calcification appeared in GA-NAV group,and the calcium contents at 2nd,4th and 8th week were (22.39 ± 1.79),(93.05 ± 6.08) and (174.97 ± 8.34) μg/mg respectively.Mild calcification occurred in GA-DAV group,and the calcium contents at 2nd,4th and 8th week were (5.57 ± 2.11),(31.15 ± 5.16),and (72.54 ± 6.50) μg/mg respectively.No obvious calcification was observed in PEG group,and the calcium contents in PEG group at 2nd,4th and 8th week were (0.46 ±0.07),(1.68 ±0.10) and (2.92 ±0.25) μg/mg,respectively.There was significant difference between two groups at the same time points (P ＜ 0.05).Conclusion PEG cross-linking can remarkably inhabit the calcification of the DAVs in rabbit subcutaneous model.