CAJ | 학술논문

目的构建DNA电化学传感器用于快速检测结核杆菌的新方法.方法根据互补序列一段设计捕获探针,另一段设计信号探针,两段探针与互补链结合构建“三明治”电化学传感模式,结合酶的催化作用放大电化学信号,实现对靶序列的高灵敏检测.结果在优化条件下目标链浓度(10 nmol/L)检测结果的相对标准偏差(RSD)=5.0％(n=5)；目标DNA在浓度为50 ～ 200 pmol/L范围内与电流值呈线性相关,回归方程:电流(I) =720.4 +93.6×浓度(c),r=0.9982,检出限为1.6×10-12 mol/L.结论此方法构建的传感器具有较高的检测灵敏度与良好的特异性.
목적 구건DNA전화학전감기용우쾌속검측결핵간균적신방법.방법 근거호보서렬일단설계포획탐침,령일단설계신호탐침,량단탐침여호보련결합구건“삼명치”전화학전감모식,결합매적최화작용방대전화학신호,실현대파서렬적고령민검측.결과 재우화조건하목표련농도(10 nmol/L)검측결과적상대표준편차(RSD)=5.0％(n=5)；목표DNA재농도위50 ～ 200 pmol/L범위내여전류치정선성상관,회귀방정:전류(I) =720.4 +93.6×농도(c),r=0.9982,검출한위1.6×10-12 mol/L.결론 차방법구건적전감기구유교고적검측령민도여량호적특이성.
Objective DNA electrochemical biosensor,as a novel technology,is establised to screen tubercule bacillus.Methods Based on the specific sequence IS6110 of tuberculosis bacilli,capture probe and signal probe are corresponding to the two segments of the complementary target chain,and they are used to form "sandwich" structure with target sequence.Then horseradish peroxidase (HRP) is added to the "sandwich" structure to catalyze the reaction of substate and amplific the electrochemical signal for detection of target sequence.Results On optimum conditions,the method was used to parallel detect 10 nmol/L target DNA for five times,with relative standard deviation(RSD) 5.0％.The relationship between current and the concentration of the synthetic target complementary strand is linear in the range from 50 pmol/L to 200 pmol/L,with the detection limit of 1.6 × 10-12 mol/L.The equation can be expressed as I(nA) =720.4 + 93.6c (target sequence),r =0.9982.Conclusion The biosensor was establised for the screening of tubercle bacillus with high sensitivity and good selectivity.