CAJ | 학술논문

目的建立不同照射剂量下大鼠放射性肠炎模型及比较其各自特点,探讨其发病机制.方法雄性SD大鼠90只,随机分为正常对照A组(18只)、小剂量5GyB组(24只)、中剂量10 Gy C组(24只)及高剂量15 Gy D组(24只),直线加速器对大鼠腹部一次性照射,在照射后2、4、7d,观察大鼠的体质量变化、死亡率、回盲部细菌移位率,光镜下观察小肠组织形态学变化,检测空肠一氧化氮(NO)的含量及采用酶联免疫吸附试验(ELESA)法检测空肠黏膜促炎细胞因子白细胞介素(IL)-1β、IL-6、IL-8、肿瘤坏死因子(TNF)-α和抗炎细胞因子IL-4、IL-10、IL-1受体拮抗剂(IL-1ra)的蛋白含量.结果放射后第2天,放射组均出现不同程度的精神差、进食少、腹泻、黏液血便等症状,与A组比较,B组各指标均无统计学意义(P＞0.05),而C、D组差异有统计学意义(P＜0.05),出现放射性肠炎,且D组较C组改变更大(P＜0.05)；第4天放射组各症状均加重,且D组大鼠全部死亡,与A组比较,B、C、D组各指标差异有统计学意义(P＜0.05),均存在放射性肠炎,且程度B组＜C组＜D组(P＜0.05)；第7天,C组仍存在各症状但较前缓解,与A组比较,B组各指标差异无统计学意义(P＞0.05),C组差异有统计学意义(P＜0.05),C组与B组比较差异有统计学意义(P＜0.05).结论本模型在不同的时间段均有各自典型的特点,适合放射性肠炎治疗研究的模型需要；放射性肠炎发病机制与NO及细胞因子变化存在密切的关系.
목적 건립불동조사제량하대서방사성장염모형급비교기각자특점,탐토기발병궤제.방법 웅성SD대서90지,수궤분위정상대조A조(18지)、소제량5GyB조(24지)、중제량10 Gy C조(24지)급고제량15 Gy D조(24지),직선가속기대대서복부일차성조사,재조사후2、4、7d,관찰대서적체질량변화、사망솔、회맹부세균이위솔,광경하관찰소장조직형태학변화,검측공장일양화담(NO)적함량급채용매련면역흡부시험(ELESA)법검측공장점막촉염세포인자백세포개소(IL)-1β、IL-6、IL-8、종류배사인자(TNF)-α화항염세포인자IL-4、IL-10、IL-1수체길항제(IL-1ra)적단백함량.결과 방사후제2천,방사조균출현불동정도적정신차、진식소、복사、점액혈편등증상,여A조비교,B조각지표균무통계학의의(P＞0.05),이C、D조차이유통계학의의(P＜0.05),출현방사성장염,차D조교C조개변경대(P＜0.05)；제4천방사조각증상균가중,차D조대서전부사망,여A조비교,B、C、D조각지표차이유통계학의의(P＜0.05),균존재방사성장염,차정도B조＜C조＜D조(P＜0.05)；제7천,C조잉존재각증상단교전완해,여A조비교,B조각지표차이무통계학의의(P＞0.05),C조차이유통계학의의(P＜0.05),C조여B조비교차이유통계학의의(P＜0.05).결론 본모형재불동적시간단균유각자전형적특점,괄합방사성장염치료연구적모형수요；방사성장염발병궤제여NO급세포인자변화존재밀절적관계.
Objective To establish and compare acute radiation enteritis models in rats with different irradiation doses,and study the pathogenesis.Methods Ninety male SD rats were divided randomly into four groups:group A (control group:n =18),group B (low dose with 5 Gy:n =24),group C (medium dose with 10 Gy:n =24),group D (high dose with 15 Gy:n =24).After a single irradiation on rat' s abdomen using linear accelerator,weight,mortality and bacterial translocation of ileocecum after irradiation at 2nd,4th and 7th day were observed.Morphologic indexes were measured by using light microscopy.Nitric oxide (NO) concentration of jejunum was measured.Protein contents of proinflammatory cytokines [interleukin (IL)-1 β,IL-6,IL-8,tumor necrosis factor (TNF)-α] and anti-inflammatory cytokines (IL-4,IL-10,IL-1ra) were examined by ELESA.Results At day 2,all irradiated groups had mental status with less eating,diarrhea and bloody stools with mucus.There were no significant differences in all indexes between groups A and B (P ＞ 0.05).However there were significant differences between group C or group D and group A (P ＜ 0.05).The changes were more severe in group D than in group C (P ＜ 0.05).At day 4,the symptoms of all irradiated groups were more severe and the rats in group D were all died.The indexes in groups B,C and D were significantly different from those in group A (P ＜ 0.05).The severity of existed radioactive enteritis was group B ＜ group C ＜ group D(P ＜ 0.05).At day 7,there still existed all symptoms in group C,but they were alleviated.There was no significant difference in all indexes between group A and group B (P ＞ 0.05),but there were significant difference between group A and group C (P ＜ 0.05),and between group C and group B (P ＜ 0.05).Conclusion In our radiation enteritis models,there were different characters at different time points after irradiation,which is suitable for study on radioactive enteritis.Pathogenesis of radiation enteritis has close relationship with cytokines and NO.