CAJ | 학술논문

目的观察肾透明细胞癌(ccRCC)与正常肾组织中ALS2CL基因的表达及差异,并探讨其意义.方法采用实时定量聚合酶链反应(PCR)、免疫组织化学(IPH)及Western blot检测26例配对癌组织／癌旁正常肾组织标本及2株ccRCC来源细胞(786/O、SN12-PM6)中ALS2CL基因mRNA和蛋白的表达.结果比较正常对照组,ALS2CL基因mRNA在65.38％ (17/26)的ccRCC组织中表达显著下调,在786/O、SN12-PM6中表达亦显著下调;IPH结果示73.08％ (19/26) ccRCC组织ALS2CL蛋白呈阴性或弱阳性表达;Western blot结果显示53.58％ (14/26)ccRCC组织ALS2CL蛋白表达下调,两株细胞ALS2CL蛋白表达亦下调,比较各自对照组,其表达差异均有统计学意义(P＜0.05).结论 ALS2CL基因表达缺失或下调可能与肾透明细胞癌发生密切相关.
목적 관찰신투명세포암(ccRCC)여정상신조직중ALS2CL기인적표체급차이,병탐토기의의.방법 채용실시정량취합매련반응(PCR)、면역조직화학(IPH)급Western blot검측26례배대암조직／암방정상신조직표본급2주ccRCC래원세포(786/O、SN12-PM6)중ALS2CL기인mRNA화단백적표체.결과 비교정상대조조,ALS2CL기인mRNA재65.38％ (17/26)적ccRCC조직중표체현저하조,재786/O、SN12-PM6중표체역현저하조;IPH결과시73.08％ (19/26) ccRCC조직ALS2CL단백정음성혹약양성표체;Western blot결과현시53.58％ (14/26)ccRCC조직ALS2CL단백표체하조,량주세포ALS2CL단백표체역하조,비교각자대조조,기표체차이균유통계학의의(P＜0.05).결론 ALS2CL기인표체결실혹하조가능여신투명세포암발생밀절상관.
Objective To study the differential expression of ALS2CL in clear cell renal cell carcinoma (ccRCC) and normal kidney tissue,and its implication.Methods Real-time polymerase chain reaction (PCR),immunohistochemistry and Western blotting were applied to examine the expression of ALS2CL in 26 cases of ccRCC tissues and adjacent normal kidney tissues as well as two kinds of ccRCCderived cell lines (786/O,SN12-PM6).Results As compared with normal control,the two ccRCC-derived cell lines (786/O,SN12-PM6) and 65.38％ (17/26) of ccRCC tissues showed an obvious downregulated expression of ALS2CL mRNA.IPH revealed that 73.08％ (19/26) of ccRCC specimens were negative or weakly positive for ALS2CL protein.Western blotting also showed a highly down-regulated rate of 53.85％ (14/26) in ccRCC samples,and the low expression of ALS2CL was also found in 786/O and SN12-PM6 cells.All of them showed significant difference between ccRCC and corresponding normal control,P ＜ 0.05.Conclusion The absence or downregulated expression of ALS2CL may be closely related to the occurrence of ccRCC.