CAJ | 학술논문

目的探讨中国汉族人群半胱天冬酶8(CASP8)基因-652 6N ins/del多态性与肾透明细胞癌(CCRCC)易感性的相关性.方法采用以医院为基础的病例-对照研究,通过问卷调查获取并采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法检测年龄和性别匹配的中国汉族300例患者和300例正常人群的CASP8-652 ins/del基因型.并采用分层分析进一步探讨与罹患CCRCC相关的可能因素.结果与ins/ins基因型携带者比较,ins/del基因型(OR =0.78,95％CI=0.55 ～1.09)或del/del基因型(0.34,0.14～0.83)携带者发生CCRCC的危险性明显降低.分层分析显示性别、体质量指数及饮酒与CCRCC危险性之间无明显相关,携带ins/del或del/del基因型的年轻非吸烟患者发生CCRCC的危险性明显降低(年龄:0.56,0.35～0.88;非吸烟:0.56,0.37～0.85).结论 CASP8-652 ins/del多态性可能与我国汉族人群CCRCC易感性有关,ins/del基因型或del/del基因型携带者发生CCRCC的危险性要明显低于ins/ins基因型携带者.
목적 탐토중국한족인군반광천동매8(CASP8)기인-652 6N ins/del다태성여신투명세포암(CCRCC)역감성적상관성.방법 채용이의원위기출적병례-대조연구,통과문권조사획취병채용취합매련반응-한제성편단장도다태성(PCR-RFLP)방법검측년령화성별필배적중국한족300례환자화300례정상인군적CASP8-652 ins/del기인형.병채용분층분석진일보탐토여리환CCRCC상관적가능인소.결과 여ins/ins기인형휴대자비교,ins/del기인형(OR =0.78,95％CI=0.55 ～1.09)혹del/del기인형(0.34,0.14～0.83)휴대자발생CCRCC적위험성명현강저.분층분석현시성별、체질량지수급음주여CCRCC위험성지간무명현상관,휴대ins/del혹del/del기인형적년경비흡연환자발생CCRCC적위험성명현강저(년령:0.56,0.35～0.88;비흡연:0.56,0.37～0.85).결론 CASP8-652 ins/del다태성가능여아국한족인군CCRCC역감성유관,ins/del기인형혹del/del기인형휴대자발생CCRCC적위험성요명현저우ins/ins기인형휴대자.
Objective To investigate the association between the-652 ins/del polymorphism in the caspases 8 (CASP8) gene and renal cell carcinoma (RCC) risk in Chinese Han population.Methods We obtained information on demographic factors by a personal interview.The polymorphism was analyzed by using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method using genomic DNA isolated from 300 renal cell cancer (RCC) patients and 300 age-and sex-matched controls.Stratification analyses by age,sex,smoking,drinking and BMI status were performed.Results As compared with the-652 ins/ins genotype,individuals carrying ins/del genotype (OR =0.78,95％ CI =0.55-1.09) or del/del genotype (0.34,0.14-0.83) had a decreased risk of RCC.Stratified analysis revealed that the increased risk was more pronounced among younger patients (0.56,0.35-0.88) and nonsmokers (0.56,0.37-0.85).Conclusion Our data suggest that CASP8-652 ins/del polymorphism may contribute to the etiology of renal cell carcinoma in Chinese Han population.