CAJ | 학술논문

目的观察大鼠骨髓间充质干细胞(BMSCs)经肾包膜下移植对可复性单侧输尿管梗阻模型(R-UUO)大鼠肾间质纤维化及纤维化过程中炎性反应的影响,探讨包膜下移植的安全性.方法 54只4月龄SD大鼠随机分为实验组(R-UUO+ MSCs)、对照组(R-UUO+ DMEM)、损伤对照组(R-UUO),每组18只,肾包膜下注射法移植后,于3、7、14 d随机于每组内取6只大鼠,取患肾.免疫组织化学检测单核／巨噬细胞表面特异性标志抗原(ED-1)表达,Masson染色检测胶原沉积量.结果 (1)和对照组(0.000773±0.000380)比较,实验组(0.000384±0.000407)在第14天的ED-1表达水平显著降低(P＜0.05),但胶原沉积量未见改善.(2)与损伤对照组(0.000293±0.000281)比较,对照组(0.000773±0.000380)在第14天时ED-1表达显著增加(P＜0.05),但胶原沉积量未见有显著增加.结论 MSCs包膜下移植可抑制肾间质纤维化过程中的巨噬细胞浸润,但短期内不能改善纤维化.包膜下注射短期内有明确损伤,但并未造成纤维化水平加重.
목적 관찰대서골수간충질간세포(BMSCs)경신포막하이식대가복성단측수뇨관경조모형(R-UUO)대서신간질섬유화급섬유화과정중염성반응적영향,탐토포막하이식적안전성.방법 54지4월령SD대서수궤분위실험조(R-UUO+ MSCs)、대조조(R-UUO+ DMEM)、손상대조조(R-UUO),매조18지,신포막하주사법이식후,우3、7、14 d수궤우매조내취6지대서,취환신.면역조직화학검측단핵／거서세포표면특이성표지항원(ED-1)표체,Masson염색검측효원침적량.결과 (1)화대조조(0.000773±0.000380)비교,실험조(0.000384±0.000407)재제14천적ED-1표체수평현저강저(P＜0.05),단효원침적량미견개선.(2)여손상대조조(0.000293±0.000281)비교,대조조(0.000773±0.000380)재제14천시ED-1표체현저증가(P＜0.05),단효원침적량미견유현저증가.결론 MSCs포막하이식가억제신간질섬유화과정중적거서세포침윤,단단기내불능개선섬유화.포막하주사단기내유명학손상,단병미조성섬유화수평가중.
Objective To investigate the efficacy and safety of mesenchymal stem cells (MSCs)subcapsular injection in R-UUO model of rats by observing the changes in macrophage infiltration and collagen deposition.Methods A total of 54 S-D rats were randomly divided into three groups (n =18 each),including the MSCs delivered group,DMEM delivered group and R-UUO group.At the day 3,7 and 14 after subcapsular transplantation of MSCs or vehicle,the 4 μm thick sections of the kidney were stained with the Masson trichrome technique.Immunohistochemical staining was performed with mouse anti-CD68 antibody (ED-1).Results (1) The subcapsular transplantation of MSCs significantly reduced the mean IA of ED-1 expression at 14th day after transplantation (P ＜ 0.05),but didn ' t attenuate the collagen deposition; (2) The subcapsular transplantation of MSCs caused a significant rise in ED-1 mean IA as compared with sham-operated group on the 14th day (P ＜ 0.05),but didn' t augment the interstitial fibrosis.Conclusion The subcapsular transplantation of MSCs in R-UUO rats could ameliorate the macrophage infiltration in interstitium,but didn' t improve the interstitial fibrosis within 2 weeks.The subcapsular transplantation of MSCs aggravates inflammation of obstructive nephropathy on the 14th day after transplantation,but doesn' t contribute to the interstitial fibrosis.