中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2012年
12期
2419-2421,封3
,共4页
急性肾损伤%脂肪间充质干细胞%细胞移植
急性腎損傷%脂肪間充質榦細胞%細胞移植
급성신손상%지방간충질간세포%세포이식
Acute kidney disease%Adipose-derived mesenchymal stem cells%Cell transplantation
目的 观察大鼠注射自体脂肪间充质干细胞(ADMSCs)对缺血再灌注(IR)损伤的保护作用.方法 将24只成年雄性SD大鼠随机分为3组:A组(假手术正常组)、B组(IR+培养基)、C组(IR+ ADMSCs),IR后1h尾静脉注射剂量为1.0×106的自体ADMSCs.缺血时间1h,再灌注72 h,接着处死大鼠观察.结果 72 h后,C组血清肌酐水平为(0.718 ±0.003) mg/dl,B组血清肌酐水平为(1.277±0.011) mg/dl,C组24h内尿量为(57.742 ±0.012) ml,B组24 h内尿量为(23.665±0.027) ml,C组尿蛋白／肌酐的比值为1.217±0.033,B组比值为1.672±0.013,各指标两组比较差异有统计学意义(P＜0.05),C组肾组织异常程度相对于B组明显减轻;Western blot法分析显示C组抗氧化标志物苯醌氧化还原酶基因(NQO1)和血红素氧合酶-1(HO-1)的蛋白表达水平显著高于B组(P＜0.05).结论 ADMSCs通过抑制氧化应激减轻IR诱导的肾损伤.
目的 觀察大鼠註射自體脂肪間充質榦細胞(ADMSCs)對缺血再灌註(IR)損傷的保護作用.方法 將24隻成年雄性SD大鼠隨機分為3組:A組(假手術正常組)、B組(IR+培養基)、C組(IR+ ADMSCs),IR後1h尾靜脈註射劑量為1.0×106的自體ADMSCs.缺血時間1h,再灌註72 h,接著處死大鼠觀察.結果 72 h後,C組血清肌酐水平為(0.718 ±0.003) mg/dl,B組血清肌酐水平為(1.277±0.011) mg/dl,C組24h內尿量為(57.742 ±0.012) ml,B組24 h內尿量為(23.665±0.027) ml,C組尿蛋白／肌酐的比值為1.217±0.033,B組比值為1.672±0.013,各指標兩組比較差異有統計學意義(P＜0.05),C組腎組織異常程度相對于B組明顯減輕;Western blot法分析顯示C組抗氧化標誌物苯醌氧化還原酶基因(NQO1)和血紅素氧閤酶-1(HO-1)的蛋白錶達水平顯著高于B組(P＜0.05).結論 ADMSCs通過抑製氧化應激減輕IR誘導的腎損傷.
목적 관찰대서주사자체지방간충질간세포(ADMSCs)대결혈재관주(IR)손상적보호작용.방법 장24지성년웅성SD대서수궤분위3조:A조(가수술정상조)、B조(IR+배양기)、C조(IR+ ADMSCs),IR후1h미정맥주사제량위1.0×106적자체ADMSCs.결혈시간1h,재관주72 h,접착처사대서관찰.결과 72 h후,C조혈청기항수평위(0.718 ±0.003) mg/dl,B조혈청기항수평위(1.277±0.011) mg/dl,C조24h내뇨량위(57.742 ±0.012) ml,B조24 h내뇨량위(23.665±0.027) ml,C조뇨단백／기항적비치위1.217±0.033,B조비치위1.672±0.013,각지표량조비교차이유통계학의의(P＜0.05),C조신조직이상정도상대우B조명현감경;Western blot법분석현시C조항양화표지물분곤양화환원매기인(NQO1)화혈홍소양합매-1(HO-1)적단백표체수평현저고우B조(P＜0.05).결론 ADMSCs통과억제양화응격감경IR유도적신손상.
Objective To observe the protective effects of autologous adipose-derived mesenchymal stem cells (ADMSCs) on the kidney in rats during renal ischemia reperfusion (IR) injury.Methods Adult male Sprague-DaWley (SD) rats (n =24) were equally randoinized into group A (sham control),group B (IR plus culture medium only),and group C (IR plus intravenous administration of 1.0 x 106 autologous ADMSCs 1 h after IR).The duration of ischemia was 1 h,followed by 72 h of reperfusion before the animals were sacrificed.Results After 72 h,serum creatinine levels in groups C and B were (0.718 ±0.003) and (1.277 ± 0.011) mg/dL,the urine volume in group C and B was (57.742 ± 0.012) and (23.665 ±0.027) mL,the ratio of protein and creatinine in group C and B was 1.217 ±0.033 and 1.672 ±0.013 respectively,and the comparison of all above items showed statistically significan difference (all P ＜ 0.05) ; Also,apparently,the renal pathohistological lesions were aggravated in group B as compared with those in group C.Western blotting showed notably higher NAD(P) H quinone oxidoreductase 1 (NQO1) and heme oxygenase-1 (HO-1) protein expression in group C than in group B.Conclusion ADMSCs therapy minimized kidney damage after IR injury through suppressing oxidative stress.
