CAJ | 학술논문

目的观察壳聚糖应用于输精管节育的生物相容性.方法用4个月龄雄性Wistar 大鼠60只,对壳聚糖进行急性毒性、亚急性毒性、溶血及输精管置入等实验.结果急性毒性实验组体质量为(251.4±16.8)g,与对照组比较差异无统计学意义(P＞0.05).亚急性毒性实验组体质量、血红蛋白、白细胞、淋巴细胞及中性粒细胞比率分别为(252.0±16.1)g、(13.80±1.37) g/dl、(8.68±0.36)×109/L、(76.60±6.81)％、(8.60±3.64)％,与对照组比较差异均无统计学意义(P＞0.05);组织和细胞未见变性坏死.溶血实验组溶血率为0.37％,小于公认的5.00％.输精管置人实验显示炎性反应较轻.结论壳聚糖应用于输精管节育的生物相容性良好.
목적 관찰각취당응용우수정관절육적생물상용성.방법 용4개월령웅성Wistar 대서60지,대각취당진행급성독성、아급성독성、용혈급수정관치입등실험.결과 급성독성실험조체질량위(251.4±16.8)g,여대조조비교차이무통계학의의(P＞0.05).아급성독성실험조체질량、혈홍단백、백세포、림파세포급중성립세포비솔분별위(252.0±16.1)g、(13.80±1.37) g/dl、(8.68±0.36)×109/L、(76.60±6.81)％、(8.60±3.64)％,여대조조비교차이균무통계학의의(P＞0.05);조직화세포미견변성배사.용혈실험조용혈솔위0.37％,소우공인적5.00％.수정관치인실험현시염성반응교경.결론 각취당응용우수정관절육적생물상용성량호.
Objective To evaluate the biocompatibility of the chitosan used in the vas deferens fertility control.Methods In 60 4-month-old male Wistar rats,acute and subacute toxicity test,hemolysis and the vas deferens plantation test were used to explore the biocompatibility of chitosan.Results In acute toxicity test,there was no significant difference in body weight between experimental group and control group (P ＞ 0.05).In subacute toxicity test,the body weight,HB,WBC,lymphocytes and neutrophils ratios in experimental group were (252.0 ± 16.1) g,(13.80 ± 1.37) g/dL,(8.68 ±0.36) x 109/L,(76.60 ± 6.81) ％ and (8.60 ± 3.64) ％,showing no significant differences from control group (P ＞0.05).Tissue and cell degeneration,and necrosis were not seen.The hemolytic rate in hemolytic experimental group was 0.37 percent,less than accepted 5.00 percent.Vas deferens plantation experiment showed a minor inflammatory response.Conclusion Chitosan using in the vas deferens fertility control has good biocompatibility.