中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2012年
12期
2437-2438
,共2页
口腔黏膜上皮细胞%种子细胞%组织工程%犬
口腔黏膜上皮細胞%種子細胞%組織工程%犬
구강점막상피세포%충자세포%조직공정%견
Oral mucosal epithelial cell%Seeding cells%Tissue engineering%Canine
目的 探讨犬上皮细胞原代培养的可行性,并从中分选出一类上皮细胞亚群.方法 酶消化法获取口腔黏膜上皮细胞,流式分选整合素α6+/CD71-的细胞亚群,观察其生物学特性,对照组细胞未用流式分选.结果 成功获取犬上皮细胞,细胞亚群形态一致,可见角蛋白丝和桥粒结构,克隆形成率(11.63％)高于对照组(6.83％),生长周期也更长;第3代亚群细胞整合素α6阳性表达率(92.3％)高于对照组(86.4％),且整合素β1荧光强度也明显增强.结论 犬上皮细胞原代培养成功,并分选出一种新型、优质的种子细胞.
目的 探討犬上皮細胞原代培養的可行性,併從中分選齣一類上皮細胞亞群.方法 酶消化法穫取口腔黏膜上皮細胞,流式分選整閤素α6+/CD71-的細胞亞群,觀察其生物學特性,對照組細胞未用流式分選.結果 成功穫取犬上皮細胞,細胞亞群形態一緻,可見角蛋白絲和橋粒結構,剋隆形成率(11.63％)高于對照組(6.83％),生長週期也更長;第3代亞群細胞整閤素α6暘性錶達率(92.3％)高于對照組(86.4％),且整閤素β1熒光彊度也明顯增彊.結論 犬上皮細胞原代培養成功,併分選齣一種新型、優質的種子細胞.
목적 탐토견상피세포원대배양적가행성,병종중분선출일류상피세포아군.방법 매소화법획취구강점막상피세포,류식분선정합소α6+/CD71-적세포아군,관찰기생물학특성,대조조세포미용류식분선.결과 성공획취견상피세포,세포아군형태일치,가견각단백사화교립결구,극륭형성솔(11.63％)고우대조조(6.83％),생장주기야경장;제3대아군세포정합소α6양성표체솔(92.3％)고우대조조(86.4％),차정합소β1형광강도야명현증강.결론 견상피세포원대배양성공,병분선출일충신형、우질적충자세포.
Objective To explore the method of primary culture of canine epithelial cells,and to sort a subset of cell groups.Methods Oral mucosa was dissociated into single cell suspensions with enzyme.Cell subsets (integrin α6 +/CD71-) were isolated by using flow cytometry,and their biological characteristics were observed.The control group was not sorted by flow cytometry.Results Epithelial cells were successfully isolated and cultured.Cell subsets had similar morphological characteristics,and keratin filaments and desmosome structure could be seen.Compared with the control group (6.83％),clone formation rate of cell subpopulations was 11.63％,and cell supopulations also showed a longer growth period.Integrin α6 expression rate (92.3％) in the third generation of cell subsets was higher than that in the control group (86.4％),and integrin β1 fluorescence intensity was significantly higher too.Conclusion Canine epithelial cells could be successfully obtained by primary culture,and we sorted out a kind of new and good seeding cells.
