CAJ | 학술논문

目的观察洛铂(LBP)对人胃癌细胞株(SGC-7901)增殖、凋亡及Metadherin(MTDH)基因表达的影响.方法采用噻唑蓝(MTT)比色法检测LBP对SGC-7901细胞的杀伤抑制作用;流式细胞仪检测SGC-7901细胞的凋亡;逆转录-聚合酶链反应(RT-PCR)和Western blot法检测MTDHmRNA及其蛋白的表达.结果 LBP可抑制胃癌SGC-7901的生长增殖,呈剂量-时间双效应关系(P ＜0.05);LBP作用胃癌SGc-7901细胞48 h后出现凋亡,且随着浓度的升高凋亡率也越来越高(P＜0.05).不同浓度的LBP作用下的胃癌SGC-7901细胞中MTDH mRNA的表达明显减弱,且呈剂量梯度下降,0、5、10、20 mg/L的LBP作用于胃癌SGC-7901细胞后的MTDH蛋白表达分别为:1.046±0.072、0.704 ±0.108、0.657±0.069、0.384±0.057,差异均有统计学意义(P＜0.05).结论 LBP可能通过抑制MTDH表达途径来抑制人胃癌SGC-7901细胞增殖并促进其凋亡.
목적 관찰락박(LBP)대인위암세포주(SGC-7901)증식、조망급Metadherin(MTDH)기인표체적영향.방법 채용새서람(MTT)비색법검측LBP대SGC-7901세포적살상억제작용;류식세포의검측SGC-7901세포적조망;역전록-취합매련반응(RT-PCR)화Western blot법검측MTDHmRNA급기단백적표체.결과 LBP가억제위암SGC-7901적생장증식,정제량-시간쌍효응관계(P ＜0.05);LBP작용위암SGc-7901세포48 h후출현조망,차수착농도적승고조망솔야월래월고(P＜0.05).불동농도적LBP작용하적위암SGC-7901세포중MTDH mRNA적표체명현감약,차정제량제도하강,0、5、10、20 mg/L적LBP작용우위암SGC-7901세포후적MTDH단백표체분별위:1.046±0.072、0.704 ±0.108、0.657±0.069、0.384±0.057,차이균유통계학의의(P＜0.05).결론 LBP가능통과억제MTDH표체도경래억제인위암SGC-7901세포증식병촉진기조망.
Objective To investigate The effect of lobaplatin (LBP) on proliferation,apoptosis and Metadherin (MTDH) expression in human gastric cancer SGC-7901 cells,and the possible mechanism.Methods Methyl thiazol tetrazolium (MTT) method was used to detect antiproliferative rate of LBP on SGC-7901 cells.Flow cytometry was used to analyze the gastric cancer cell apoptosis.Reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting were used to examine the expression of MTDH at mRNA and protein levels respectively.Results LBP significantly exhibited the cell growth inhibition in the SGC-7901 cells in a time-and concentration-dependent manner (P ＜ 0.05).LBP significantly induced apoptosis in SGC-7901 cells in a dose-dependent manner (P ＜ 0.05).MTDH expression was significantly decreased by LBP at mRNA levels,and MTDH protein levels in SGC-7901 cells treated with 0,5,10 and 20 mg/L LBP were 1.046 ±0.072,0.704 ±0.108,0.657 ±0.069 and 0.384 ±0.057 respectively (P ＜ 0.05).Conclusion LBP can inhibit proliferation of SGC-7901 ceils and induce their apoptosis in vitroprobably by suppressing the MTDH expression pathway.