CAJ | 학술논문

目的探讨化疗药物5-氟尿嘧啶(5-Fu)在结肠癌细胞SW480和肝癌细胞HepG2对免疫共抑制分子B7-H1表达的诱导及机制.方法 5-Fu处理细胞后24 h用免疫荧光和流式细胞仪检测5-Fu对B7-H1蛋白表达的影响,在3～24h用荧光定量聚合酶链反应(RT-qPCR)检测其mRNA水平.结果 5-Fu(10 μg/L)处理这两种细胞24h后可探测出增强的B7-H1免疫荧光信号,流式细胞仪检测发现5-Fu处理SW480细胞后B7-H1蛋白含量为(14.88±1.43)％,处理HepG2细胞后B7-H1蛋白含量为(18.29±1.11)％,与各自对照组比较差异均有统计学意义(P＜0.05).然而RT-qPCR检测表明在5-Fu处理后3～24h,SW480和HepG细胞内B7-H1 mRNA的相对含量相对恒定.与各自对照组比较差异均无统计学意义(P＞0.05).结论 5-Fu在治疗肿瘤的同时会引起B7-H1蛋白的表达,但这种增加与mRNA分子的转录无关.
목적 탐토화료약물5-불뇨밀정(5-Fu)재결장암세포SW480화간암세포HepG2대면역공억제분자B7-H1표체적유도급궤제.방법 5-Fu처리세포후24 h용면역형광화류식세포의검측5-Fu대B7-H1단백표체적영향,재3～24h용형광정량취합매련반응(RT-qPCR)검측기mRNA수평.결과 5-Fu(10 μg/L)처리저량충세포24h후가탐측출증강적B7-H1면역형광신호,류식세포의검측발현5-Fu처리SW480세포후B7-H1단백함량위(14.88±1.43)％,처리HepG2세포후B7-H1단백함량위(18.29±1.11)％,여각자대조조비교차이균유통계학의의(P＜0.05).연이RT-qPCR검측표명재5-Fu처리후3～24h,SW480화HepG세포내B7-H1 mRNA적상대함량상대항정.여각자대조조비교차이균무통계학의의(P＞0.05).결론 5-Fu재치료종류적동시회인기B7-H1단백적표체,단저충증가여mRNA분자적전록무관.
Objective To study the mechanism of 5-fluorouracil (5-Fu) inducing B7-H1 in SW480 and HepG2 ceils.Methods The B7-H1 protein expression was detected by using immunofluorescence and flow cytometry in SW480 and HepG2 cells at 24 h after 5-Fu treatment.The expression of B7-H1 mRNA was detected by using real-time quantitative polymerase chain reaction (RT-qPCR) 3-24 h after 5-Fu treatment.Results Immunofluorescence revealed a stronger fluorescence signal of BT-H1 in SW480 and HepG2 cells treated with 5-Fu (10 μg/L) for 24 h.Flow cytometry demonstrated B7-H1 protein in SW480 cells and HepG2 ceils treated with 5-Fu treatment was (14.88 ± 1.43)％ and (18.29 ± 1.11)％respectively,which was significantly different from that in control group (P ＜ 0.05).Quantitative real-time PCR showed that the mRNA expression of B7-H1 was stable in SW480 and HepG2 cells 3-24 h after 5-Fu treatment.Conclusion 5-Fu could cause increased protein expression of B7-H1,which was not related with the increased mRNA expression.