中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2012年
12期
2563-2565
,共3页
口腔鳞癌%粒细胞集落刺激生物因子%白细胞介素-4
口腔鱗癌%粒細胞集落刺激生物因子%白細胞介素-4
구강린암%립세포집락자격생물인자%백세포개소-4
Oral squamous cell carcinoma%Granulocyte macrophage-colony stimulating factor%Interleukin-4
目的 观察白细胞介素-4(IL-4)和粒细胞集落刺激因子(GM-CSF)对肿瘤浸润淋巴细胞的体外培养和细胞毒效应的影响.方法 将提取自肿瘤标本的肿瘤浸润淋巴细胞(TIL)设为A、B、C3组,均采用浓度为10％的RPMI 1640培养基,其中C组仅加入浓度为900 U/ml的IL-2,A组在C组基础上加入浓度为900 U/ml的IL-4,B组在C组基础上加入浓度为100 mg/L的GM-CSF,37℃恒温,8％ CO2环境中培养,绘制生长曲线图,选择各组中处于对数生长期的细胞利用噻唑蓝(MTT)比色法检测其杀瘤活性.结果 C组增殖550倍,A组增殖2810倍,B组增殖3550倍.C组TIL细胞24d进入对数生长期,A组和B组第8天即进入,A组和B组的TIL细胞增殖速度和最后的增殖量均明显大于C组(P＜0.05).杀瘤活性:A组为(55.34 ±0.05)％,B组为(56.47±0.08)％,均强于C组的(25.61±0.07)％ (P ＜0.05).结论 IL-4和GM-CSF能与IL-2协同作用提高TIL细胞在体外培养的增殖速度,能有效提高其杀瘤活性.
目的 觀察白細胞介素-4(IL-4)和粒細胞集落刺激因子(GM-CSF)對腫瘤浸潤淋巴細胞的體外培養和細胞毒效應的影響.方法 將提取自腫瘤標本的腫瘤浸潤淋巴細胞(TIL)設為A、B、C3組,均採用濃度為10％的RPMI 1640培養基,其中C組僅加入濃度為900 U/ml的IL-2,A組在C組基礎上加入濃度為900 U/ml的IL-4,B組在C組基礎上加入濃度為100 mg/L的GM-CSF,37℃恆溫,8％ CO2環境中培養,繪製生長麯線圖,選擇各組中處于對數生長期的細胞利用噻唑藍(MTT)比色法檢測其殺瘤活性.結果 C組增殖550倍,A組增殖2810倍,B組增殖3550倍.C組TIL細胞24d進入對數生長期,A組和B組第8天即進入,A組和B組的TIL細胞增殖速度和最後的增殖量均明顯大于C組(P＜0.05).殺瘤活性:A組為(55.34 ±0.05)％,B組為(56.47±0.08)％,均彊于C組的(25.61±0.07)％ (P ＜0.05).結論 IL-4和GM-CSF能與IL-2協同作用提高TIL細胞在體外培養的增殖速度,能有效提高其殺瘤活性.
목적 관찰백세포개소-4(IL-4)화립세포집락자격인자(GM-CSF)대종류침윤림파세포적체외배양화세포독효응적영향.방법 장제취자종류표본적종류침윤림파세포(TIL)설위A、B、C3조,균채용농도위10％적RPMI 1640배양기,기중C조부가입농도위900 U/ml적IL-2,A조재C조기출상가입농도위900 U/ml적IL-4,B조재C조기출상가입농도위100 mg/L적GM-CSF,37℃항온,8％ CO2배경중배양,회제생장곡선도,선택각조중처우대수생장기적세포이용새서람(MTT)비색법검측기살류활성.결과 C조증식550배,A조증식2810배,B조증식3550배.C조TIL세포24d진입대수생장기,A조화B조제8천즉진입,A조화B조적TIL세포증식속도화최후적증식량균명현대우C조(P＜0.05).살류활성:A조위(55.34 ±0.05)％,B조위(56.47±0.08)％,균강우C조적(25.61±0.07)％ (P ＜0.05).결론 IL-4화GM-CSF능여IL-2협동작용제고TIL세포재체외배양적증식속도,능유효제고기살류활성.
Objective To observe the efficacy of interleukin-4 (IL-4) and granulocyte macrophage-colony stimulating factor (GM-CSF) on the proliferation and cytotoxicity of tumor infiltrating lymphocytes (TIL).Methods TILs were divided into 3 groups,in which RPMI 1640 (10％) medium was used.Groups C,A and B were given IL-2 (900 U/mL),IL-2 (900 U/L) +IL-4 (900 U/mL),and IL-2 (900 U/L) + IL-4 (900 U/mL) + GM-CSF (100 mg/L) respectively.Growth curves were drawn.The TILs in logarithmic growth phase in the 3 groups were selected for measurement of tumor-cytotoxic effect by using methyl thiazol tetrazolium (MTT) assay.Results The final number of cells was 550 times greater than the initial number in group C,while 2810 times in group A,and 3550 times in group B.The cells reached the logarithmic growth phase at 24th day in group C,while at 8th day in both groups A and B.The speed and final amount of cell proliferation were increased in groups A and B as compared with group C (P ＜ 0.05).The MTT assay revealed that the tumor-cytotoxic effect of TILs in groups A and B was (55.34 ± 0.05) ％ and (56.47 ± 0.08) ％,stronger than that in group C [(25.61 ± 0.07) ％] (P ＜ 0.05).Conclusion IL-4 and GM-CSF could increase the proliferation of TILs.Both of them could also enhance the cytotoxicity of TILs.
