CAJ | 학술논문

目的探讨RhoA蛋白在食管癌组织中的表达水平及其临床病理意义.方法应用免疫组织化学链霉亲和素-生物素-过氧化物酶复合物(SABC)法检测140例食管癌组织中RhoA蛋白的表达水平,并分析RhoA蛋白在食管癌组织中的表达水平与临床病理特征以及患者预后之间的关系.采用逆转录-聚合酶链反应(RT-PCR)检测RhoA mRNA在30例食管癌组织及其配对的癌旁组织中的相对表达水平.结果 RhoA蛋白在140例食管癌组织中的阳性率为60.7％,其高表达水平与食管癌患者的肿瘤浸润程度、淋巴结转移、TNM分期密切相关(P＜0.05);Kaplan-Meier单因素生存分析显示RhoA蛋白高表达组患者的5年无病生存率(6.0％)和总生存率(18.1％)显著小于低表达组患者的22.6％和39.6％(P＜0.05);Cox回归模型显示RhoA蛋白表达在无病生存率和总生存率中危险比为1.669和1.638,是食管癌患者的一个独立危险因素(P＜0.05).RhoA mRNA在食管癌组织中表达(0.49±0.21)较配对的癌旁组织中表达(0.28±0.19)明显增高(P＜0.01).结论 RhoA在人类食管癌中高表达,其在食管癌的发生和发展过程中起重要作用,与肿瘤侵袭及转移密切相关.
목적 탐토RhoA단백재식관암조직중적표체수평급기림상병리의의.방법 응용면역조직화학련매친화소-생물소-과양화물매복합물(SABC)법검측140례식관암조직중RhoA단백적표체수평,병분석RhoA단백재식관암조직중적표체수평여림상병리특정이급환자예후지간적관계.채용역전록-취합매련반응(RT-PCR)검측RhoA mRNA재30례식관암조직급기배대적암방조직중적상대표체수평.결과 RhoA단백재140례식관암조직중적양성솔위60.7％,기고표체수평여식관암환자적종류침윤정도、림파결전이、TNM분기밀절상관(P＜0.05);Kaplan-Meier단인소생존분석현시RhoA단백고표체조환자적5년무병생존솔(6.0％)화총생존솔(18.1％)현저소우저표체조환자적22.6％화39.6％(P＜0.05);Cox회귀모형현시RhoA단백표체재무병생존솔화총생존솔중위험비위1.669화1.638,시식관암환자적일개독립위험인소(P＜0.05).RhoA mRNA재식관암조직중표체(0.49±0.21)교배대적암방조직중표체(0.28±0.19)명현증고(P＜0.01).결론 RhoA재인류식관암중고표체,기재식관암적발생화발전과정중기중요작용,여종류침습급전이밀절상관.
Objective To explore the expression and clinical significance of RhoA in oesophageal cancer.Methods The expression of RhoA protein in 140 specimens of primary oesophageal cancer tissues was detected by using immunohistochemical strept avidin-biotin complex (SABC) staining,and its correlation with clinicopathological parameters and prognosis of patients was analyzed.The expression of RhoA mRNA in 30 cases of primary oesophageal cancer tissues and their paired adjacent non-cancer tissues was evaluated by using reverse transcription-polymerase chain reaction (RT-PCR).Results The positive rate of RhoA protein was 60.7％,and the high expression of RhoA protein was significantly associated with depth of invasion (T),lymph node metastasis (N),and clinical TNM stage respectively (P ＜ 0.05).Kaplan-Meier univariate survival analysis revealed that five-year disease-free survival and overall survival rate for patients with high expression of RhoA protein were 6.0％ and 18.1％ respectively,which were significantly lower than 22.6％ and 39.6％ in patients with low expression of RhoA protein (P ＜ 0.05).Multivariate Cox regression analysis revealed that RhoA expression was an independent prognostic factor in disease-free survival and overall survival for patients with oesophageal cancer (Hazard ratio was 1.669 and 1.638 respectively,P ＜ 0.05).The expression of RhoA mRNA was detectable in 30 cases of primary oesophageal cancer tissues (0.49 ±0.21) and their paired adjacent non-cancer tissues (0.28 ±0.19),but the expression in oesophageal cancer tissues was significantly higher than that in paired adjacent non-cancer tissues (P ＜ 0.01).Conclusion High expression of RhoA was detectably in oesophageal cancer,suggesting its important role in the malignant tumorigenesis and progression of oesophageal cancer,and its close correlation with invasion and metastasis of oesophageal cancer.