中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2012年
12期
2590-2591,封4
,共3页
王亚东%胡学宁%张冬蕾%王思博%朱少平
王亞東%鬍學寧%張鼕蕾%王思博%硃少平
왕아동%호학저%장동뢰%왕사박%주소평
特发性气胸%空气细微颗粒%肺泡巨噬细胞%单核细胞趋化蛋白-1
特髮性氣胸%空氣細微顆粒%肺泡巨噬細胞%單覈細胞趨化蛋白-1
특발성기흉%공기세미과립%폐포거서세포%단핵세포추화단백-1
Idiopathic pneumothorax%Fine particulate mater%Alveolar macrophage%Monocyte chemoattractant protein-1
目的 探讨空气细微颗粒与青年特发性气胸的关系.方法 收集武汉大学中南医院胸心外科2011年3月至2011年9月治疗的20例青年特发性气胸患者肺大疱组织标本作为实验组,10例青年正常肺组织标本作为对照组,采用苏木素-伊红(HE)染色法、Wright-Giemsa (W-G)染色法和酶联免疫吸附试验(ELISA)法分别检测肺泡巨噬细胞(AM)、空气细微颗粒及单核细胞趋化蛋白-1(MCP-1)在两组肺组织中的表达.结果 实验组可见空气细微颗粒物明显沉积,而对照组肺组织中极少见空气细微颗粒物;实验组AM的百分比为(30.100±3.636)％,对照组为(14.260±4.312)％,AM在实验组表达明显高于对照组(P＜0.05);实验组肺组织中MCP-1表达水平为(1.191×103±184.812) ng/L,对照组为(0.540×103±125.058) ng/L,MCP-1在实验组中表达明显增强(P＜0.05).结论 青年特发性气胸肺组织中存在空气细微颗粒,此颗粒刺激气道上皮细胞引发炎性反应,激活AM产生大量的MCP-1,进而促进和加重气道炎性反应,结果提示青年特发性气胸的发生与空气细微颗粒沉积有关.
目的 探討空氣細微顆粒與青年特髮性氣胸的關繫.方法 收集武漢大學中南醫院胸心外科2011年3月至2011年9月治療的20例青年特髮性氣胸患者肺大皰組織標本作為實驗組,10例青年正常肺組織標本作為對照組,採用囌木素-伊紅(HE)染色法、Wright-Giemsa (W-G)染色法和酶聯免疫吸附試驗(ELISA)法分彆檢測肺泡巨噬細胞(AM)、空氣細微顆粒及單覈細胞趨化蛋白-1(MCP-1)在兩組肺組織中的錶達.結果 實驗組可見空氣細微顆粒物明顯沉積,而對照組肺組織中極少見空氣細微顆粒物;實驗組AM的百分比為(30.100±3.636)％,對照組為(14.260±4.312)％,AM在實驗組錶達明顯高于對照組(P＜0.05);實驗組肺組織中MCP-1錶達水平為(1.191×103±184.812) ng/L,對照組為(0.540×103±125.058) ng/L,MCP-1在實驗組中錶達明顯增彊(P＜0.05).結論 青年特髮性氣胸肺組織中存在空氣細微顆粒,此顆粒刺激氣道上皮細胞引髮炎性反應,激活AM產生大量的MCP-1,進而促進和加重氣道炎性反應,結果提示青年特髮性氣胸的髮生與空氣細微顆粒沉積有關.
목적 탐토공기세미과립여청년특발성기흉적관계.방법 수집무한대학중남의원흉심외과2011년3월지2011년9월치료적20례청년특발성기흉환자폐대포조직표본작위실험조,10례청년정상폐조직표본작위대조조,채용소목소-이홍(HE)염색법、Wright-Giemsa (W-G)염색법화매련면역흡부시험(ELISA)법분별검측폐포거서세포(AM)、공기세미과립급단핵세포추화단백-1(MCP-1)재량조폐조직중적표체.결과 실험조가견공기세미과립물명현침적,이대조조폐조직중겁소견공기세미과립물;실험조AM적백분비위(30.100±3.636)％,대조조위(14.260±4.312)％,AM재실험조표체명현고우대조조(P＜0.05);실험조폐조직중MCP-1표체수평위(1.191×103±184.812) ng/L,대조조위(0.540×103±125.058) ng/L,MCP-1재실험조중표체명현증강(P＜0.05).결론 청년특발성기흉폐조직중존재공기세미과립,차과립자격기도상피세포인발염성반응,격활AM산생대량적MCP-1,진이촉진화가중기도염성반응,결과제시청년특발성기흉적발생여공기세미과립침적유관.
Objective To observe the relationship between fine particulate matter and idiopathic spontaneous pneumothorax in youngs.Methods Twenty young patients with idiopathic spontaneous pneumothorax as experimental group were randomly collected from March 2011 to September 2011,and ten normal lung tissue specimens served as normal control group.The lung tissue sections stained by hematoxylin and eosin (HE) and Wright-Giemsa (W-G) respectively were examined in terms of alveolar macrophage (AM) and fine particulate matters.The level of MCP-1 in the lung tissue was measured by using enzyme linked immunosorbent assay (ELISA).Results Fine particulate matters were found obviously in experimental group,but rarely seen in normal control group.The percentage of AM in experimental group and normal control group was (30.100 ± 3.636) ％ and (14.260 ± 4.312) ％ respectively.MCP-1 levels in serum were (1.191 x 103 ± 184.812) ng/L and (0.540 x 103 ± 125.058) ng/L in experimental group and normal control group respectively.AM counts and MCP-1 levels in experimental group were significantly higher than those in normal control group (P ＜ 0.05).Conclusion In the lung tissues of the idiopathic spontaneous pneumothorax in youngs,there exist fine particulate matters,which can stimulate the airway epithelial cells and cause inflammatory responses.At the same time they can stimulate mononuclear macrophage to produce amounts of MCP-1,which may contribute to and deteriorate the airway inflammatory responses.It is believed that the deposition of fine particulate matters may be related with the occurrence of idiopathic spontaneous pneumothorax in youngs.
