CAJ | 학술논문

目的探讨肺癌细胞株及肺癌组织中神经丝蛋白轻链多肽(NEFL)的表达与肺癌患者临床病理特征和生存期之间的相关性.方法链菌素抗生物素蛋白-过氧化物酶(SP)免疫组织化学法检测108例肺癌组织中NEFL的表达,并对108例肺癌患者进行随访观察.非参数秩和检验检测两个独立样本的NEFL的表达差异;Cox比例风险模型分析预后,应用SPSS 13.0软件进行统计学分析.结果 NEFL的阳性表达率在淋巴结转移阳性和阴性的肺癌患者中,分别为9.4％和32.7％,差异有统计学意义(x20.05,1=8.74,P＜0.01),而与性别、吸烟、组织类型、分化程度、肿瘤分期、肿瘤大小无相关.Cox比例风险模型多因素预后分析显示,肿瘤患者的性别[相对危险度(RR)=-1.030,P＜0.01]、肿瘤分期(RR=0.474,P＜0.01)、淋巴结转移(RR=0.675,P＜0.05)和NEFL表达(RR=-1.147,P＜0.05)与死亡有关(P＜0.05).结论 NEFL的表达与肺癌淋巴结转移有关系,其阳性表达与患者预后呈正相关.
목적 탐토폐암세포주급폐암조직중신경사단백경련다태(NEFL)적표체여폐암환자림상병리특정화생존기지간적상관성.방법 련균소항생물소단백-과양화물매(SP)면역조직화학법검측108례폐암조직중NEFL적표체,병대108례폐암환자진행수방관찰.비삼수질화검험검측량개독립양본적NEFL적표체차이;Cox비례풍험모형분석예후,응용SPSS 13.0연건진행통계학분석.결과 NEFL적양성표체솔재림파결전이양성화음성적폐암환자중,분별위9.4％화32.7％,차이유통계학의의(x20.05,1=8.74,P＜0.01),이여성별、흡연、조직류형、분화정도、종류분기、종류대소무상관.Cox비례풍험모형다인소예후분석현시,종류환자적성별[상대위험도(RR)=-1.030,P＜0.01]、종류분기(RR=0.474,P＜0.01)、림파결전이(RR=0.675,P＜0.05)화NEFL표체(RR=-1.147,P＜0.05)여사망유관(P＜0.05).결론 NEFL적표체여폐암림파결전이유관계,기양성표체여환자예후정정상관.
Objective To investigate the neurofilament light chain gene (NEFL) expression of lung cancer cells and the relationship between clinicopathological characteristics and NEFL expression.The prognostic significance of NEFL expression in human lung cancer was studied also.Methods NEFL expression was examined immunohistochemically in paraffin-embedded tissue specimens from 108 patients with lung cancer.The mRNA expression levels of NEFL transcripts in lung cancer cell lines were quantitatively assessed by real-time reverse transcriptase polymerase chain reaction (PCR).The 108 patients were followed up from 1 to 48 months with a median follow-up duration of 29.8 months.Then the association among the expression of NEFL and clinicopathalogic variables and overall survival of lung cancer was analyzed by using SPSS 13.0 software package.Results The positive rate of NEFL expression in specimens without lymph node metastasis (32.4％) was higher than that in tissues with lymph node metastasis(9.4％,x20.05,1 =8.74,P ＜ 0.01),but not correlated with gender,smoking,histologic types,differentiation,pathological grade and tumor diameter (P ＞ 0.05).Multivariate Cox proportional hazards regression analysis indicated that gender (RR,-1.030 ; P ＜ 0.01),tumor grade (RR,0.474 ; P ＜ 0.01),lymph node metastases (RR,0.675 ; P ＜ 0.05) and expression of NEFL (RR,-1.147 ; P ＜ 0.05) predicted for death (P ＜ 0.05).Conclusion The results suggest that NEFL expression was significantly associated with lymph node metastases in the samples and positively correlated with prognosis.