中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2013年
1期
17-19
,共3页
钟骏桥%向俊峰%尹航%温超%谢元康%郭旭%陈桢坤%黄约翰%张启瑜
鐘駿橋%嚮俊峰%尹航%溫超%謝元康%郭旭%陳楨坤%黃約翰%張啟瑜
종준교%향준봉%윤항%온초%사원강%곽욱%진정곤%황약한%장계유
胰腺癌%脱落酸%增殖%脱噬作用
胰腺癌%脫落痠%增殖%脫噬作用
이선암%탈락산%증식%탈서작용
Pancreatic carcinoma%Abscisic acid%Proliferation%Apoptosis
目的 探讨脱落酸(ABA)对人胰腺癌细胞株(PANC-1)增殖的影响及其作用机制.方法 细胞计数试剂盒(CCK-8)法筛选出ABA处理浓度,实验分为对照组和ABA处理组,处理组设3个处理浓度,0.001、0.010、0.100 mmol/L.通过细胞形态观察、CCK-8法及膜联蛋白V/碘化丙锭(Annexin V/PI)方法观察ABA对PANC-1增殖的影响.逆转录-聚合酶链反应(RT-PCR)及Western blot法分别检测端粒酶mRNA(hTERT mRNA)及半胱氨酰天冬氨酸特异性蛋白酶(Caspase)-3、B淋巴细胞/白血病-2(bcl-2)、细胞周期素(Cyclin) D1蛋白的表达.结果 ABA使细胞增殖减慢(24 h:F =52.253,P＜0.01;48 h:F=107.461,P＜0.01),促进凋亡(对照组和各处理组抑制率分别为4.26％、15.42％、22.02％、39.95％).处理组hTERT mRNA表达下降,Caspase-3表达增强,bcl-2及Cyclin D1表达减弱,此效应随ABA浓度升高而明显.结论 ABA能抑制PANC-1细胞增殖,促进凋亡,其机制是活化Caspase-3,降低hTERT mRNA及bcl-2、Cyclin D1蛋白的表达.
目的 探討脫落痠(ABA)對人胰腺癌細胞株(PANC-1)增殖的影響及其作用機製.方法 細胞計數試劑盒(CCK-8)法篩選齣ABA處理濃度,實驗分為對照組和ABA處理組,處理組設3箇處理濃度,0.001、0.010、0.100 mmol/L.通過細胞形態觀察、CCK-8法及膜聯蛋白V/碘化丙錠(Annexin V/PI)方法觀察ABA對PANC-1增殖的影響.逆轉錄-聚閤酶鏈反應(RT-PCR)及Western blot法分彆檢測耑粒酶mRNA(hTERT mRNA)及半胱氨酰天鼕氨痠特異性蛋白酶(Caspase)-3、B淋巴細胞/白血病-2(bcl-2)、細胞週期素(Cyclin) D1蛋白的錶達.結果 ABA使細胞增殖減慢(24 h:F =52.253,P＜0.01;48 h:F=107.461,P＜0.01),促進凋亡(對照組和各處理組抑製率分彆為4.26％、15.42％、22.02％、39.95％).處理組hTERT mRNA錶達下降,Caspase-3錶達增彊,bcl-2及Cyclin D1錶達減弱,此效應隨ABA濃度升高而明顯.結論 ABA能抑製PANC-1細胞增殖,促進凋亡,其機製是活化Caspase-3,降低hTERT mRNA及bcl-2、Cyclin D1蛋白的錶達.
목적 탐토탈락산(ABA)대인이선암세포주(PANC-1)증식적영향급기작용궤제.방법 세포계수시제합(CCK-8)법사선출ABA처리농도,실험분위대조조화ABA처리조,처리조설3개처리농도,0.001、0.010、0.100 mmol/L.통과세포형태관찰、CCK-8법급막련단백V/전화병정(Annexin V/PI)방법관찰ABA대PANC-1증식적영향.역전록-취합매련반응(RT-PCR)급Western blot법분별검측단립매mRNA(hTERT mRNA)급반광안선천동안산특이성단백매(Caspase)-3、B림파세포/백혈병-2(bcl-2)、세포주기소(Cyclin) D1단백적표체.결과 ABA사세포증식감만(24 h:F =52.253,P＜0.01;48 h:F=107.461,P＜0.01),촉진조망(대조조화각처리조억제솔분별위4.26％、15.42％、22.02％、39.95％).처리조hTERT mRNA표체하강,Caspase-3표체증강,bcl-2급Cyclin D1표체감약,차효응수ABA농도승고이명현.결론 ABA능억제PANC-1세포증식,촉진조망,기궤제시활화Caspase-3,강저hTERT mRNA급bcl-2、Cyclin D1단백적표체.
Objective To probe the effects of abscisic acid (ABA) on proliferation of human pancreatic cancer cell line (PANC-1) and the mechanisms.Methods The processing concentration of ABA was screened out by cell counting kit-8 (CCK-8) assay.The experiment was divided into control group and ABA-treated groups (0.001,0.01,0.1 mmol/L).The effects of ABA on proliferation of PANC-1 cells were observed by using morphologic examination,CCK-8 assay and Annexin V/PI staining.The expression of hTERT mRNA was detected by using reverse transcription-polymerase chain reaction (RT-PCR).The expressions of Caspase-3,B lymphocytes/leukemia-2 (bcl-2) and Cyclin D1 was detected by using Western blotting.Results ABA slowed down proliferation of PANC-1 cells (24 h:F =52.253,P ＜ 0.01 ; 48 h:F =107.461,P ＜0.01) as compared with control group.ABA could promote apoptosis of PANC-1 cells (The inhibitory rate in control group and ABA groups was respectively 4.26％,15.42％,22.02％,and 39.95％).The expression of hTERT mRNA was decreased,the expression of Caspase-3 was increased,while bcl-2 and Cyclin D1 were decreased in ABA groups.The effects were concentration-dependent.Conclusion ABA can inhibit proliferation and promote apoptosis of PANC-1 cells probably by activating Caspase-3,and reducing the expression of hTERT mRNA,bcl-2 and Cyclin D1.
