中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2013年
1期
23-25,封3
,共4页
朱武晖%杨波%王卫星%余佳%邓文宏%赵凯亮%石乔
硃武暉%楊波%王衛星%餘佳%鄧文宏%趙凱亮%石喬
주무휘%양파%왕위성%여가%산문굉%조개량%석교
急性胰腺炎%甲状腺滤泡上皮细胞%超微结构%功能改变
急性胰腺炎%甲狀腺濾泡上皮細胞%超微結構%功能改變
급성이선염%갑상선려포상피세포%초미결구%공능개변
Acute pancreatitis%Thyroid follicular cells%Ultrastructure%Functional change
目的 观察重症急性胰腺炎(SAP)时甲状腺滤泡上皮细胞超微结构和功能的变化.方法 雄性Wistar大鼠40只,体质量200～ 250 g(湖北省疾病预防控制中心提供),随机分为5组:假手术组(SO组)、重症急性胰腺炎模型1、3、6、12h组(SAP 1、3、6、12h组).胆胰管逆行注射5％牛磺胆酸钠制备SAP模型.测定淀粉酶(AMY)、磷脂酶A2(sPLA2)、T3、T4、游离甲状腺原氨酸(FT3)、游离甲状腺素(FT4)水平.抽血后取甲状腺腺叶组织,光镜观察病理改变,电镜下观察滤泡上皮细胞超微结构改变.结果 与SO组比较,功能上SAP组大鼠血AMY、sPLA2水平明显升高,分别为(2489±442～5253±717) U/L和(621.33 ±23.77～ 1021.11 ±54.17) mol/(min·ml) (P ＜0.05),血T3、FT3、FT4水平明显下降,结果分别为(0.55 ±0.21 ～0.76±0.28) nmol/L、(0.93±0.15 ～1.98±0.63) nmol/L和(0.63 ±0.37 ～3.29±1.49) nmol/L(P＜ 0.05),T4水平先升高后降低,结果为(20.70 ±5.75 ～42.95±19.82) nmol/L,差异有统计学意义(p＜0.05).形态学上,滤泡增生、融合,滤泡上皮细胞脱落,细胞核膜内陷,微绒毛减少,粗面内质网脱颗粒,高尔基体、线粒体肿胀,间质纤维增生,毛细血管扩张、充血.结论 机体甲状腺激素水平的改变,可作为评估SAP病情严重程度的评估指标.
目的 觀察重癥急性胰腺炎(SAP)時甲狀腺濾泡上皮細胞超微結構和功能的變化.方法 雄性Wistar大鼠40隻,體質量200～ 250 g(湖北省疾病預防控製中心提供),隨機分為5組:假手術組(SO組)、重癥急性胰腺炎模型1、3、6、12h組(SAP 1、3、6、12h組).膽胰管逆行註射5％牛磺膽痠鈉製備SAP模型.測定澱粉酶(AMY)、燐脂酶A2(sPLA2)、T3、T4、遊離甲狀腺原氨痠(FT3)、遊離甲狀腺素(FT4)水平.抽血後取甲狀腺腺葉組織,光鏡觀察病理改變,電鏡下觀察濾泡上皮細胞超微結構改變.結果 與SO組比較,功能上SAP組大鼠血AMY、sPLA2水平明顯升高,分彆為(2489±442～5253±717) U/L和(621.33 ±23.77～ 1021.11 ±54.17) mol/(min·ml) (P ＜0.05),血T3、FT3、FT4水平明顯下降,結果分彆為(0.55 ±0.21 ～0.76±0.28) nmol/L、(0.93±0.15 ～1.98±0.63) nmol/L和(0.63 ±0.37 ～3.29±1.49) nmol/L(P＜ 0.05),T4水平先升高後降低,結果為(20.70 ±5.75 ～42.95±19.82) nmol/L,差異有統計學意義(p＜0.05).形態學上,濾泡增生、融閤,濾泡上皮細胞脫落,細胞覈膜內陷,微絨毛減少,粗麵內質網脫顆粒,高爾基體、線粒體腫脹,間質纖維增生,毛細血管擴張、充血.結論 機體甲狀腺激素水平的改變,可作為評估SAP病情嚴重程度的評估指標.
목적 관찰중증급성이선염(SAP)시갑상선려포상피세포초미결구화공능적변화.방법 웅성Wistar대서40지,체질량200～ 250 g(호북성질병예방공제중심제공),수궤분위5조:가수술조(SO조)、중증급성이선염모형1、3、6、12h조(SAP 1、3、6、12h조).담이관역행주사5％우광담산납제비SAP모형.측정정분매(AMY)、린지매A2(sPLA2)、T3、T4、유리갑상선원안산(FT3)、유리갑상선소(FT4)수평.추혈후취갑상선선협조직,광경관찰병리개변,전경하관찰려포상피세포초미결구개변.결과 여SO조비교,공능상SAP조대서혈AMY、sPLA2수평명현승고,분별위(2489±442～5253±717) U/L화(621.33 ±23.77～ 1021.11 ±54.17) mol/(min·ml) (P ＜0.05),혈T3、FT3、FT4수평명현하강,결과분별위(0.55 ±0.21 ～0.76±0.28) nmol/L、(0.93±0.15 ～1.98±0.63) nmol/L화(0.63 ±0.37 ～3.29±1.49) nmol/L(P＜ 0.05),T4수평선승고후강저,결과위(20.70 ±5.75 ～42.95±19.82) nmol/L,차이유통계학의의(p＜0.05).형태학상,려포증생、융합,려포상피세포탈락,세포핵막내함,미융모감소,조면내질망탈과립,고이기체、선립체종창,간질섬유증생,모세혈관확장、충혈.결론 궤체갑상선격소수평적개변,가작위평고SAP병정엄중정도적평고지표.
Objective To observe the ultra,structural and functional changes of the thyroid follicular cells in rats with severe acute pancreatitis (SAP) and then to provide a theoretical basis for clinical treatment of thyroid damage in patients with SAP.Methods Forty Wistar rats weighing 200-250 g were randomly divided into 5 groups:the sham operation group (SO group) and the SAP 1,3,6,12 h groups (8 rats per group).SAP model was induced by retrograde injection of 5％ sodium taurocholate into the biliopancreatic duct.AMY,sPLA2,T3,T4,FT3 and FT4 in serum were determined.The pathological and ultrastructural changes in the thyroid tissue were observed under the light microscopy and TEM respectively.Results As compared with SO group,the AMY,sPLA2,T3,FT3 and FT4 levels in SAP groups were significantly reduced in serum:(2489 ±442-5253 ±717) U/L,(621.33 ± 23.77-1021.11 ± 54.7) mol/(min· mt),(0.55 ±0.21-0.76 ±0.28) nmol/L,(0.93 ±0.15-1.98 ±0.63) nmol/Land (0.63 ±0.37-3.29 ±1.49) nmol/L (P ＜ 0.05) ; The T4 levels were increased first and then decreased:(20.70 ± 5.75-42.95 ± 19.82) nmol/L (P ＜ 0.05).Morphologically,thyroid follicles were increased and thyroid follicular cells exfoliated,nuclear membrane retracted,microvilli reduced,rough endoplasmic reticulum degranulated,Golgi apparatus and mitochondria enlarged,interstitial fibrosis increased,and capillaries were expanded and filled will blood cells.Conclusion The changes of thyroid hormones car be used to evaluate the severity of SAP.
