CAJ | 학술논문

目的观察塞来昔布对人甲状腺髓样癌裸鼠皮下种植瘤生长的抑制作用,并探讨其作用机制.方法按照人甲状腺髓样癌TT细胞2×106个/只接种在40只BALB/c裸鼠皮下制作成肿瘤动物模型,随机分成A、B、C、D4组,分别给予生理盐水、塞来昔布、阿霉素、塞来昔布联合阿霉素,其中塞来昔布每只300 mg/(kg·d),阿霉素每只2 mg/(kg·),待接种部位出现直径约0.5 cm瘤结节后连续给予相应药物治疗1周,分别测量裸鼠体质量、肿瘤质量,3周后处死裸鼠取瘤体计算抑瘤率,采用免疫组织化学法检测肿瘤组织及肝、肺组织中环氧合酶-2(COX-2)、基质金属蛋白酶-9(MMP-9)、血管内皮生长因子(VEGF)的表达.结果 A、B、C、D组的抑瘤率分别为0％、45.37％、49.71％、69.68％,B、C、D组与A组比较(P＜0.05),其余各组间抑瘤率比较差异无统计学意义(P＞0.05).COX-2、MMP-9、VEGF在A组中的表达明显高于B、C、D组,差异有统计学意义(P＜0.05),三者在B、C、D组中的表达差异均无统计学意义(P＞0.05).在各实验组肝组织中均出现肿瘤细胞的侵袭,COX-2、MMP-9、VEGF均呈现阳性.结论塞来昔布能抑制甲状腺髓样癌皮下种植瘤的生长,联合阿霉素能增强抑瘤效果.
목적 관찰새래석포대인갑상선수양암라서피하충식류생장적억제작용,병탐토기작용궤제.방법 안조인갑상선수양암TT세포2×106개/지접충재40지BALB/c라서피하제작성종류동물모형,수궤분성A、B、C、D4조,분별급여생리염수、새래석포、아매소、새래석포연합아매소,기중새래석포매지300 mg/(kg·d),아매소매지2 mg/(kg·),대접충부위출현직경약0.5 cm류결절후련속급여상응약물치료1주,분별측량라서체질량、종류질량,3주후처사라서취류체계산억류솔,채용면역조직화학법검측종류조직급간、폐조직중배양합매-2(COX-2)、기질금속단백매-9(MMP-9)、혈관내피생장인자(VEGF)적표체.결과 A、B、C、D조적억류솔분별위0％、45.37％、49.71％、69.68％,B、C、D조여A조비교(P＜0.05),기여각조간억류솔비교차이무통계학의의(P＞0.05).COX-2、MMP-9、VEGF재A조중적표체명현고우B、C、D조,차이유통계학의의(P＜0.05),삼자재B、C、D조중적표체차이균무통계학의의(P＞0.05).재각실험조간조직중균출현종류세포적침습,COX-2、MMP-9、VEGF균정현양성.결론 새래석포능억제갑상선수양암피하충식류적생장,연합아매소능증강억류효과.
Objective To observe the inhibitory effect of celecoxib on the growth of transplant tumor of medullary thyroid carcinoma in nude mice.Methods Forty mice were randomly divided into 4 groups (A,B,C and D):control group,celecoxib group,doxorubicin group and celecoxib ± doxorubicin group.All vaccination animal models were established by subcutaneous injection of 2 × 106 of human medullary thyroid carcinoma TT cells.The celecoxib was given 300 mg/(kg·d),and doxorubicin 2 mg/(kg·d).Tumor volumes and weight of mice were measured one week after administration when 0.5 cm tumor nodus appeared in the injection region,and tumor inhibition rate was calculated after 3 weeks.The expression levels of cyclooxegenase-2 (COX-2),matrix metalloproteinases-9 (MMP-9) and vascular endothelial growth factor (VEGF) in the vaccination tumor,liver and lung tissues were detected by using immunohistochemistry,Results The inhibition rate in groups A,B,C,and D was 0％,45.37％,49.71％,and 69.68％ respectively,and the inhibitory effects in groups B,C and D were increased as compared with group A (P ＜ 0.05),but there was no obvious difference among groups B,C and D.The expression levels of COX-2,MMP-9 and VEGF in group A were obviously higher.than in groups B,C and D (P ＜ 0.05),but there was no significant difference among groups B,C and D (P ＞ 0.05).In groups B,C and D,invasion of tumor cells in the liver and lung tissues was seen,and the expression of COX-2,MMP-9 and VEGF was positive.Conclusion Celecoxib can inhibit the growth of vaccination tumors of medullary thyroid carcinoma,and combination of celecoxib with doxorubicin can exert the synergistic effects.