CAJ | 학술논문

目的探讨Cullin1(Cul1)基因对乳腺癌细胞迁移及侵袭的影响及机制.方法 Cul1小干扰RNA(siRNA)、对照siRNA分别转染人乳腺癌MDA-MB-231和BT-549细胞,细胞迁移试验观察迁移能力；细胞侵袭试验观察侵袭能力；明胶酶谱实验检测细胞分泌基质金属蛋白酶(MMPs)的能力；Western blot法检测MMPs及组织金属蛋白酶抑制剂(TIMPs)蛋白的表达.结果与对照组比较,Cul1 siRNA处理组MDA-MB-231和BT-549两种细胞的迁移能力分别减少了83％和77％；侵袭能力分别减少了84％和79％；MMP-2活力分别减少了88％和76％;MMP-2蛋白表达分别减少了47.87％和15.35％,TIMP-2蛋白表达量分别增加了49.03％和68.84％,差异均有统计学意义(P＜0.05).结论 Cul1基因干涉后通过上调TIMP-2表达进而抑制MMP-2表达及分泌,最终抑制乳腺癌细胞的侵袭能力.
목적 탐토Cullin1(Cul1)기인대유선암세포천이급침습적영향급궤제.방법 Cul1소간우RNA(siRNA)、대조siRNA분별전염인유선암MDA-MB-231화BT-549세포,세포천이시험관찰천이능력；세포침습시험관찰침습능력；명효매보실험검측세포분비기질금속단백매(MMPs)적능력；Western blot법검측MMPs급조직금속단백매억제제(TIMPs)단백적표체.결과 여대조조비교,Cul1 siRNA처리조MDA-MB-231화BT-549량충세포적천이능력분별감소료83％화77％；침습능력분별감소료84％화79％；MMP-2활력분별감소료88％화76％;MMP-2단백표체분별감소료47.87％화15.35％,TIMP-2단백표체량분별증가료49.03％화68.84％,차이균유통계학의의(P＜0.05).결론 Cul1기인간섭후통과상조TIMP-2표체진이억제MMP-2표체급분비,최종억제유선암세포적침습능력.
Objective To investigate the role of Cullin1 (Cul1) gene in human breast cancer cel1 migration and invasion and its molecular mechanisms.Methods The Cul1 small interfering RNA (siRNA)was transfected into MDA-MB-231 and BT-549 breast cancer cells.The migration abilities of two breast cancer cel lines were examined by cell migration assay.The invasive abilities were tested by using cell invasion assay.The matrix metalloproteinase (MMPs) activities were detected by using gelatin zymography,and the expression of MMPs and tissue inhibitor of metalloproteinase (TIMPs) proteins was detected by using Western blotting.Results Compared to the control siRNA treatment,Cul1 interference could drastically decreased cell migration abilities of both cell lines by 83％ and 77％,and the cell invasive abilities of both cell lines by 83％ and 77％,respectively.The MMP-2 activity was significantly suppressed after interference of Cul1 in the two breast cancer cell lines by 88％ and 76％,respectively,compared to the control cells.The expression levels of MMP-2 protein were drastically decreased by 88％ and 76％ in the two breast cancer cell lines after Cul1 interference,but TIMP-2 protein expression level was significantly increased by 49.03％ and 68.84％,significantly.Conclusion Silencing Cul1 could suppress human breast cancer cell invasion through up-regulation of TIMP-2 and down-regulation of MMP-2.