CAJ | 학술논문

目的观察N-(3,4-二甲氧基肉桂酰)邻氨基苯甲酸(3,4-DAA)对大鼠肝移植急性排斥反应的预防作用.方法以Wistar大鼠为供体,SD大鼠为受体,建立大鼠肝移植模型,随机分为4组.A组:对照组(n=22),B组:3,4-DAA 400 mg/(kg·d)组(n=22),C组:环孢素A(CsA)5 mg/(kg·d)组(n=22).D组:3,4-DAA 400 mg/(kg·d) +CsA 5 mg/(kg·d)组(n=22).各组于手术当天起连续口服给药,分别于术后第3、5、7天处死受体,收集血液行肝功能检测并观察肝组织病理学变化.各组另随机选4只受体观察生存时间.结果 (1)4组受体平均生存时间分别为(11.3±1.7)、(16.0±0.8)、(21.5±1.7)、(26.5±1.3)d,B组与A组比较生存时间延长,差异有统计学意义(P＜0.01)；D组受体存活时间最长,与A、B、C3组比较,差异均有统计学意义(P＜0.0l).(2)B组大鼠术后各时间外周血谷丙转氨酶(ALT)、谷草转氨酶(AST)、总红胆素(T-BIL)值均低于A组(P＜0.05).D组各时间点ALl、AST、T-BIL值低于A、B、C组(P＜0.05).(3)术后第7天肝脏排斥活动指数B组明显低于A组(P＜0.05),D组得分最低,与A、B、C组比较,差异有统计学意义(P＜0.05).结论 3,4-DAA能抑制大鼠肝移植术后急性排斥反应,与CsA合用具有协同作用.
목적 관찰N-(3,4-이갑양기육계선)린안기분갑산(3,4-DAA)대대서간이식급성배척반응적예방작용.방법 이Wistar대서위공체,SD대서위수체,건립대서간이식모형,수궤분위4조.A조:대조조(n=22),B조:3,4-DAA 400 mg/(kg·d)조(n=22),C조:배포소A(CsA)5 mg/(kg·d)조(n=22).D조:3,4-DAA 400 mg/(kg·d) +CsA 5 mg/(kg·d)조(n=22).각조우수술당천기련속구복급약,분별우술후제3、5、7천처사수체,수집혈액행간공능검측병관찰간조직병이학변화.각조령수궤선4지수체관찰생존시간.결과 (1)4조수체평균생존시간분별위(11.3±1.7)、(16.0±0.8)、(21.5±1.7)、(26.5±1.3)d,B조여A조비교생존시간연장,차이유통계학의의(P＜0.01)；D조수체존활시간최장,여A、B、C3조비교,차이균유통계학의의(P＜0.0l).(2)B조대서술후각시간외주혈곡병전안매(ALT)、곡초전안매(AST)、총홍담소(T-BIL)치균저우A조(P＜0.05).D조각시간점ALl、AST、T-BIL치저우A、B、C조(P＜0.05).(3)술후제7천간장배척활동지수B조명현저우A조(P＜0.05),D조득분최저,여A、B、C조비교,차이유통계학의의(P＜0.05).결론 3,4-DAA능억제대서간이식술후급성배척반응,여CsA합용구유협동작용.
Objective To investigate the role of N-(3',4'-dimethoxycinnamonyl) anthranilic acid (3,4-DAA) in the prevention of acute rejection after orthotopic liver transplantation in rats.Methods The Wistar-SD rat orthotopic liver transplantation model was established and divided into four groups:group A (control group),given normal saline (2 ml/d) orally after operation; group B,given 3,4-DAA at dose of 400 mg/(kg·d) ; group C,given cyclosporin A (CsA) at 5 mg/(kg·d) ; group D,given 3,4-DAA at 400 mg/(kg· d) and CsA at 5 mg/(kg· d).The samples (peripheral blood and liver tissue) were obtained at 3rd,5th and 7th day post-operation.Pathological study and serum test were performed on each sample.Four recipients were chosen randomly in each group for survival observation.Results (1) The average survival time of recipients in groups A,B,C and D was (11.3 ± 1.7),(16.0 ±0.8),(21.5 ± 1.7) and (26.5 ± 1.3) days,respectively.Recipients in group B survived longer than in group A (P ＜0.01),and those in group D survived the longest in four groups (P ＜ 0.01) ; (2) In group A,alanine aminotransferase (ALT),aspartate aminotransferase (AST) and total bilirubin (T-BIL) were dramatically increased,and the pathological examination showed severe acute rejection (AR) on the day 7.In group B,ALT,AST and T-BIL were decreased and the pathological examination showed lower RAI on the day 7 (P ＜0.05).In group D,ALT,AST and T-BIL were decreased to the normal levels and AR was uncertainly detected on the day 7 (P ＜0.05).Conclusion 3,4-DAA exerts the anti-rejection effect after liver transplantation in rats.