CAJ | 학술논문

目的观察红花对肝星状细胞c-fos和c-jun基因表达的影响.方法在培养的肝星状细胞株中分别加入不同浓度的红花(终质量浓度分别为1.0、0.5 g/L)；于8、24、48、72h4个时间点分别收集细胞,提取肝星状细胞总RNA;用逆转录-聚合酶链反应(RT-PCR)方法测定c-fos和c-jun的基因表达水平.结果红花两组HSC-T细胞c-fos基因表达水平在8 h(0.49 ±0.13、0.34±0.11)、24 h(0.42±0.10、0.26 ±0.08)、48 h(0.31 ±0.07、0.20±0.04)、72 h(0.23 ±0.05、0.12±0.04)4个时间点均明显低于对照组(0.63±0.13、0.50±0.12、0.43±0.06、0.32±0.04)；c-jun基因表达水平在8 h(0.70±0.14、0.46±0.11)、24 h(0.57 ±0.10、0.43±0.07)、48 h(0.42 ±0.09、0.26±0.06)、72 h(0.31 ±0.05、0.12±0.04)4个时间点也均明显低于对照组(0.93±0.13、0.80 ±0.12、0.63 ±0.10、0.46±0.07);随剂量加大,HSC-T细胞c-los、c-jun基因表达水平逐渐降低；两组间差异有统计学意义(P＜0.05).结论红花对肝星状细胞c-fos和c-jun基因表达有明显下调作用.
목적 관찰홍화대간성상세포c-fos화c-jun기인표체적영향.방법 재배양적간성상세포주중분별가입불동농도적홍화(종질량농도분별위1.0、0.5 g/L)；우8、24、48、72h4개시간점분별수집세포,제취간성상세포총RNA;용역전록-취합매련반응(RT-PCR)방법측정c-fos화c-jun적기인표체수평.결과 홍화량조HSC-T세포c-fos기인표체수평재8 h(0.49 ±0.13、0.34±0.11)、24 h(0.42±0.10、0.26 ±0.08)、48 h(0.31 ±0.07、0.20±0.04)、72 h(0.23 ±0.05、0.12±0.04)4개시간점균명현저우대조조(0.63±0.13、0.50±0.12、0.43±0.06、0.32±0.04)；c-jun기인표체수평재8 h(0.70±0.14、0.46±0.11)、24 h(0.57 ±0.10、0.43±0.07)、48 h(0.42 ±0.09、0.26±0.06)、72 h(0.31 ±0.05、0.12±0.04)4개시간점야균명현저우대조조(0.93±0.13、0.80 ±0.12、0.63 ±0.10、0.46±0.07);수제량가대,HSC-T세포c-los、c-jun기인표체수평축점강저；량조간차이유통계학의의(P＜0.05).결론 홍화대간성상세포c-fos화c-jun기인표체유명현하조작용.
Objective To investigate the effects of carthamus tinctorius on c-fos and c-jun gene expression,in hepatic stellate cells (HSCs).Methods Hepatic stellate cell' line HSC-T6 cells were cultured in medium containing different concentrations of carthamus tinctorius (1.0,and 0.5 g/L) and harvested at different incubation periods (8,24,48,and 72 h).Total RNA of HSCs was isolated,and c-fos and c-jun gene expression levels were detected by using reverse transcription polymerase chain reaction.Results C-fos gene expression levels of HSCs in two carthamus tinctorius-treated groups were significantly lower than in control group (0.63 ±0.13,0.50 ±0.12,0.43 ±0.06,0.32 ±0.04) after exposure to carthamus tinctorius for 8 h (0.49 ±0.13,0.34 ±0.11),24 h (0.42 ±0.10,0.26 ±0.08),48 h (0.31 ±0.07,0.20 ±0.04),and 72 h (0.23 ± 0.05,0.12 ± 0.04).C-jun gene expression levels of HSCs in two carthamus tinctorius-treated groups were also significantly lower than in control group (0.93 ±0.13,0.80±0.12,0.63 ±0.10,0.46 ±0.07) after exposure to carthamus tinctorius at 8 h (0.70 ±0.14,0.46 ±0.11),24 h (0.57 ±0.10,0.43 ±0.07),48 h (0.42 ±0.09,0.26 ±0.06),and 72 h(0.31 ±0.05,0.12 ±0.04).The gene expression levels of c-los and c-jun in HSCs were decreased gradually as the dosage of carthamus tinctorius increased,and there were significant difference in the c-los,and c-jun gene expression between two carthamus tinctorius-treated groups.Condusion Carthamus tinctorius may down-regulate c-fos and c-jun gene expression in HSCs.