中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2013年
1期
84-85
,共2页
杨牟%张居文%陈萍%孙林%车海杰%勇俊%李鲁滨%宋富波
楊牟%張居文%陳萍%孫林%車海傑%勇俊%李魯濱%宋富波
양모%장거문%진평%손림%차해걸%용준%리로빈%송부파
肢体缺血%缺血后适应%缺血再灌注%急性肠缺血
肢體缺血%缺血後適應%缺血再灌註%急性腸缺血
지체결혈%결혈후괄응%결혈재관주%급성장결혈
Limb ischemia%Ischemic postconditioning%Ischemia-reperfusion injury%Acute intestinal ischemia
目的 探讨肢体缺血后适应对兔急性肠道缺血再灌注损伤的保护作用及其机制.方法 健康雄性新西兰大白兔36只,随机分为3组(每组12只):空白对照组(Con)、肠道缺血再灌注组(IRI)、肢体缺血后适应组(LIP).再灌注结束后测定血清中丙二醛(MDA)含量,测定肠道组织中髓过氧化物酶(MPO)活性并在光镜下观察肠道组织形态学变化.结果 IRI组和LIP组的MDA含量[(8.93±1.25)、(5.32±1.54),mol/L]、MPO活性[(3.68±0.45)、(1.94±0.51) U/100 g]及肠黏膜损伤评分(4.15±0.81、2.96±0.64)均明显高于Con组[(3.96±1.36) μmol/L、(0.56±0.36) U/100 g、0.82±0.70),差异均有统计学意义(P＜0.01).LIP组的MDA含量[(5.32±1.54) μmol/L]、MPO活性[(1.94±0.51) U/100 g]及肠黏膜损伤评分(2.96±0.64)明显轻于IRI组[(8.93±1.25) μmol/L、(3.68±0.45) U/100 g、4.15 ±0.81],差异有统计学意义(P＜0.01).结论 肠道缺血再灌注前肢体短暂缺血再灌注对肠道急性缺血再灌注损伤有显著保护作用.其保护作用可能与减轻活性氧的损伤及加强抗氧化能力有关.
目的 探討肢體缺血後適應對兔急性腸道缺血再灌註損傷的保護作用及其機製.方法 健康雄性新西蘭大白兔36隻,隨機分為3組(每組12隻):空白對照組(Con)、腸道缺血再灌註組(IRI)、肢體缺血後適應組(LIP).再灌註結束後測定血清中丙二醛(MDA)含量,測定腸道組織中髓過氧化物酶(MPO)活性併在光鏡下觀察腸道組織形態學變化.結果 IRI組和LIP組的MDA含量[(8.93±1.25)、(5.32±1.54),mol/L]、MPO活性[(3.68±0.45)、(1.94±0.51) U/100 g]及腸黏膜損傷評分(4.15±0.81、2.96±0.64)均明顯高于Con組[(3.96±1.36) μmol/L、(0.56±0.36) U/100 g、0.82±0.70),差異均有統計學意義(P＜0.01).LIP組的MDA含量[(5.32±1.54) μmol/L]、MPO活性[(1.94±0.51) U/100 g]及腸黏膜損傷評分(2.96±0.64)明顯輕于IRI組[(8.93±1.25) μmol/L、(3.68±0.45) U/100 g、4.15 ±0.81],差異有統計學意義(P＜0.01).結論 腸道缺血再灌註前肢體短暫缺血再灌註對腸道急性缺血再灌註損傷有顯著保護作用.其保護作用可能與減輕活性氧的損傷及加彊抗氧化能力有關.
목적 탐토지체결혈후괄응대토급성장도결혈재관주손상적보호작용급기궤제.방법 건강웅성신서란대백토36지,수궤분위3조(매조12지):공백대조조(Con)、장도결혈재관주조(IRI)、지체결혈후괄응조(LIP).재관주결속후측정혈청중병이철(MDA)함량,측정장도조직중수과양화물매(MPO)활성병재광경하관찰장도조직형태학변화.결과 IRI조화LIP조적MDA함량[(8.93±1.25)、(5.32±1.54),mol/L]、MPO활성[(3.68±0.45)、(1.94±0.51) U/100 g]급장점막손상평분(4.15±0.81、2.96±0.64)균명현고우Con조[(3.96±1.36) μmol/L、(0.56±0.36) U/100 g、0.82±0.70),차이균유통계학의의(P＜0.01).LIP조적MDA함량[(5.32±1.54) μmol/L]、MPO활성[(1.94±0.51) U/100 g]급장점막손상평분(2.96±0.64)명현경우IRI조[(8.93±1.25) μmol/L、(3.68±0.45) U/100 g、4.15 ±0.81],차이유통계학의의(P＜0.01).결론 장도결혈재관주전지체단잠결혈재관주대장도급성결혈재관주손상유현저보호작용.기보호작용가능여감경활성양적손상급가강항양화능력유관.
Objective To investigate the protective effect of remote postconditioning induced by a single 10-min episode femoral artery occlusion referfusion applied immediately before the oneset of superior mesenteric artery perfusion on acute intestinal ischemia-reperfusion injury (IRI).Methods Thirty-six healthy New Zealand white rabbits were randomly divided into three groups (n =12 in each group):control,IRI,and limb ischemic postconditioning (LIP).Tissue myeloperoxidase (MPO) activity and malondialdehyde (MDA) levels were determined and the morphological changes in intestinal tissues were observed under the microscopy.Results MDA levels,MPO activity and the score of intestinal mucosal injury were significandy increased in IRI and LIP groups as compared with control group (P ＜ 0.01).MDA levels and MPO activity were significantly reduced in RLP group [(5.32 ± 1.54) μmol/L,and (1.94 ±0.51) U/100 g] as compared with IRI group [(8.93 ± 1.25) μmol/L,and (3.68 ± 0.45) U/100 g] (P ＜ 0.01).The score of intestinal mucosal injury in LIP group was lower than in IRI group (2.96 ± 0.64 vs.4.15 ±0.81).Conclusion Limb postconditioning protects the mice from acute IRI probable by decreasing the injury caused by oxygen free radicals and increasing the capability of antioxidation.
