中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2013年
1期
93-95,封3
,共4页
高广荣%张成%吕晨光%张宝磊%张雪峰
高廣榮%張成%呂晨光%張寶磊%張雪峰
고엄영%장성%려신광%장보뢰%장설봉
失血性休克%低温复苏%过氧化物酶体增殖物激活受体-γ%诱导性一氧化氮合酶%肿瘤坏死因子-α
失血性休剋%低溫複囌%過氧化物酶體增殖物激活受體-γ%誘導性一氧化氮閤酶%腫瘤壞死因子-α
실혈성휴극%저온복소%과양화물매체증식물격활수체-γ%유도성일양화담합매%종류배사인자-α
Hemorrhagic shock%Hypothermia resuscitation%Peroxisome proliferator activated receptor gamma%Inducible nitric oxide synthase%Tumor necrosis factor-α
目的 观察失血性休克大鼠在不同温度复苏后肺组织内过氧化物酶体增殖物激活受体-γ(PPAR-γ)、诱导性一氧化氮合酶(iNOS)和肿瘤坏死因子-α(TNF-α)基因表达的变化.方法 36只成年雄性Wistar大鼠随机平均分为3组:对照组(S)、休克常温复苏组(NR) (37 ～38℃)和低温复苏组(HR) (33 ～34℃).S组只进行外科插管操作,不建立失血性休克模型及复苏,NR组和HR组在建立失血性休克模型后分别在预定温度下进行复苏.实时定量聚合酶链反应(Real-time PCR)检测复苏60 min和240 min大鼠肺组织PPAR-γ、iNOS和TNF-α mRNA表达变化.结果 (1)与对照组比较,复苏60 min NR组和HR组PPAR-γ mRNA表达量分别为0.61±0.09和1.71±0.16(P＜0.05).复苏240 min NR组和HR组PPAR-γ mRNA表达量分别为0.54±0.08和2.82±0.19(P＜0.05)；(2)与对照组比较,复苏60 min NR组和HR组iNOS mRNA表达量分别为3.43±0.27和2.21±0.28(P ＜0.05).复苏240 min NR组和HR组iNOS mRNA表达量分别为4.86±0.62和2.93±0.37(P ＜0.05)；(3)与对照组比较,复苏60 min NR组和HR组TNF-α mRNA表达量分别为4.12±0.30和3.36±0.34(P ＜0.05).复苏240 min NR组和HR组TNF-α mRNA表达量分别为 5.87±0.34和4.91±0.53(P ＜0.05).结论 低温复苏能够上调失血性休克大鼠肺组织PPAR-γ基因表达,抑制TNF-α和iNOS基因表达,减轻肺组织损伤.
目的 觀察失血性休剋大鼠在不同溫度複囌後肺組織內過氧化物酶體增殖物激活受體-γ(PPAR-γ)、誘導性一氧化氮閤酶(iNOS)和腫瘤壞死因子-α(TNF-α)基因錶達的變化.方法 36隻成年雄性Wistar大鼠隨機平均分為3組:對照組(S)、休剋常溫複囌組(NR) (37 ～38℃)和低溫複囌組(HR) (33 ～34℃).S組隻進行外科插管操作,不建立失血性休剋模型及複囌,NR組和HR組在建立失血性休剋模型後分彆在預定溫度下進行複囌.實時定量聚閤酶鏈反應(Real-time PCR)檢測複囌60 min和240 min大鼠肺組織PPAR-γ、iNOS和TNF-α mRNA錶達變化.結果 (1)與對照組比較,複囌60 min NR組和HR組PPAR-γ mRNA錶達量分彆為0.61±0.09和1.71±0.16(P＜0.05).複囌240 min NR組和HR組PPAR-γ mRNA錶達量分彆為0.54±0.08和2.82±0.19(P＜0.05)；(2)與對照組比較,複囌60 min NR組和HR組iNOS mRNA錶達量分彆為3.43±0.27和2.21±0.28(P ＜0.05).複囌240 min NR組和HR組iNOS mRNA錶達量分彆為4.86±0.62和2.93±0.37(P ＜0.05)；(3)與對照組比較,複囌60 min NR組和HR組TNF-α mRNA錶達量分彆為4.12±0.30和3.36±0.34(P ＜0.05).複囌240 min NR組和HR組TNF-α mRNA錶達量分彆為 5.87±0.34和4.91±0.53(P ＜0.05).結論 低溫複囌能夠上調失血性休剋大鼠肺組織PPAR-γ基因錶達,抑製TNF-α和iNOS基因錶達,減輕肺組織損傷.
목적 관찰실혈성휴극대서재불동온도복소후폐조직내과양화물매체증식물격활수체-γ(PPAR-γ)、유도성일양화담합매(iNOS)화종류배사인자-α(TNF-α)기인표체적변화.방법 36지성년웅성Wistar대서수궤평균분위3조:대조조(S)、휴극상온복소조(NR) (37 ～38℃)화저온복소조(HR) (33 ～34℃).S조지진행외과삽관조작,불건립실혈성휴극모형급복소,NR조화HR조재건립실혈성휴극모형후분별재예정온도하진행복소.실시정량취합매련반응(Real-time PCR)검측복소60 min화240 min대서폐조직PPAR-γ、iNOS화TNF-α mRNA표체변화.결과 (1)여대조조비교,복소60 min NR조화HR조PPAR-γ mRNA표체량분별위0.61±0.09화1.71±0.16(P＜0.05).복소240 min NR조화HR조PPAR-γ mRNA표체량분별위0.54±0.08화2.82±0.19(P＜0.05)；(2)여대조조비교,복소60 min NR조화HR조iNOS mRNA표체량분별위3.43±0.27화2.21±0.28(P ＜0.05).복소240 min NR조화HR조iNOS mRNA표체량분별위4.86±0.62화2.93±0.37(P ＜0.05)；(3)여대조조비교,복소60 min NR조화HR조TNF-α mRNA표체량분별위4.12±0.30화3.36±0.34(P ＜0.05).복소240 min NR조화HR조TNF-α mRNA표체량분별위 5.87±0.34화4.91±0.53(P ＜0.05).결론 저온복소능구상조실혈성휴극대서폐조직PPAR-γ기인표체,억제TNF-α화iNOS기인표체,감경폐조직손상.
Objective To investigate the effects of different body temperature resuscitation on the expression of peroxisome proliferator activated receptor gamma (PPAR-γ),inducible nitric oxide synthase (iNOS) and tumor necrosis factor-α (TNF-α) genes in the lung tissue of rats with hemorrhagic shock.Methods Thirty-six adult male Wistar rats were randomly assigned to three groups:control group (S),normal thermia group (NR) and hypothermia group (HR).Rats in S control group underwent the surgical procedure but were not bled.Rats in NR group and HR group received normal thermia (37-38 ℃) resuscitation or hypothermia (33-34 ℃) resuscitation respectively during resuscitation and hourly thereafter.Realtime polymerase chain reaction (teal-time PCR) method was used to detect the mRNA expression of PPAR-γ,iNOS and TNF-α in the lung tissutes of rats with hemorrhagic shock after resuscitation for 60 min or 240min.Results (1) As compared with S group,PPAR-γ mRNA expression in NR group and HR group was0.61 ± 0.09 and 1.71 ± 0.16 respectively (P ＜ 0.05) after resuscitation for 60 min following hemorrhagic shock and 0.54 ± 0.08,and 2.82 ± 0.19 (P ＜ 0.05) after resuscitation for 240 min following hemorrhagic shock ; (2) As compared with S group,iNOS mRNA expression in NR group and HR group was 3.43 ±0.27 and 2.21 ±0.28 (P ＜0.05) after resuscitation for 60 min following hemorrhagic shock,and 4.86 ±0.62 and 2.93 ± 0.37 (P ＜ 0.05) after resuscitation for 240 min following hemorrhagic shock; (3) As compared with S group,TNF-α mRNA expression in NR group and HR group was 4.12 ±0.30 and 3.36 ±0.34 (P ＜0.05) after resuscitation for 60 min following hemorrhagic shock,and 5.87 ±0.34 and 4.91 ±0.53 (P ＜ 0.05) after resuscitation for 240 min following hemorrhagic shock.Conclusion Hypothermia resuscitation after hemorrhagic shock can raise the PPAR-γgene expression and inhibit iNOS and TNF-α gene expression in the rat lung tissues.Hypothermia resuscitation can reduce the lung tissue damage.
