CAJ | 학술논문

目的探讨A3NEP1-40以RADA-IKVAV/FRM为载体的缓释规律.方法 DMEM/F12溶液能触发RADA-IKVAV/FRM自组装,包裹A3NEP1-40形成均质水凝胶.将包裹125 μg及25 μgA3NEP1-40的水凝胶各自放入2.5ml及0.5ml磷酸盐缓冲液(PBS)中进行释放,并依此分为A、B、C、D4组.应用高效液相色谱仪检测并计算A3NEP1-40每天释放量及累计释放量,统计学分析释放规律.结果 (1)最初的24h呈爆发释放,40％的A3NEPl-40自凝胶内释出；平稳释放阶段内样本中可持续检测到A3NEP1-40释放达40d.(2)A组与B组、C组与D组差异无统计学意义,A组与C、D两组、B组与C、D两组差异有统计学意义(P＜0.05).结论 RADA-IKVAV/FRM可以控制A3NEP1-40释放速度,达到缓释效果,是一种较为理想的神经组织工程材料,同时也可以用作载体构建缓释药物.
목적 탐토A3NEP1-40이RADA-IKVAV/FRM위재체적완석규률.방법 DMEM/F12용액능촉발RADA-IKVAV/FRM자조장,포과A3NEP1-40형성균질수응효.장포과125 μg급25 μgA3NEP1-40적수응효각자방입2.5ml급0.5ml린산염완충액(PBS)중진행석방,병의차분위A、B、C、D4조.응용고효액상색보의검측병계산A3NEP1-40매천석방량급루계석방량,통계학분석석방규률.결과 (1)최초적24h정폭발석방,40％적A3NEPl-40자응효내석출；평은석방계단내양본중가지속검측도A3NEP1-40석방체40d.(2)A조여B조、C조여D조차이무통계학의의,A조여C、D량조、B조여C、D량조차이유통계학의의(P＜0.05).결론 RADA-IKVAV/FRM가이공제A3NEP1-40석방속도,체도완석효과,시일충교위이상적신경조직공정재료,동시야가이용작재체구건완석약물.
Objective To mvestigate the controlled-release law of A3NEP1-40 encapsulated in the hydrogel of RADA-IKVAV/FRM formed by self-assembly.Methods DMEM/F12 solution can trigger RADA-IKVAV/FRM self-assembly into a porous homogeneous hydrogel,and encapsulate A3NEP1-40 into the internal voids of the hydrogel via hydrophobic interaction.The hydrogels containing 125 μg or 25 μg A3NEP1-40 were put a PBS buffer to formulate a controlled-release system.Four groups were created according to the load of A3NEP1-40 and the buffer volume labeled A,B,C,D.The concentration of A3NEP1-40 in the sample was detected by using high performance liquid chromatography,and the release amount of a day and the cumulative release amount were calculated.The SPSS13.0 statistical software was used to analyze the controlled-release law of A3NEP1-40.Results (1) At the first 24 h,there was a burst release phase,approximately 40％ of the loaded A3NEP1-40 released from gel; in the steady release stage,sustainable A3NEP1-40 release could be detected in the sample for 40 days; (2) There was no statistically significant difference in the A3NEP1-40 release between group A and group B,or between group C and group D,but there was significant difference between groups A and C,groups A and D,groups B and C,or groups B and D (all P ＜ 0.05).Condusion The release speed of A3 NEP1-40 can be controlled by self-assembly nanometer polypeptide gel materials and a sustained release effect can be achieved.Self-as-sembly nanometer polypeptide hydrogel containing RADA-IKVAV/FRM is a very good controlled-release carrier,and can be applied to nerve tissue engineering to promote spinal cord injury repair.