中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2013年
1期
144-146
,共3页
赵力%牟东成%彭吉润%朱继业%黄磊%冷希圣
趙力%牟東成%彭吉潤%硃繼業%黃磊%冷希聖
조력%모동성%팽길윤%주계업%황뢰%랭희골
癌,肝细胞%肿瘤睾丸抗原%诊断
癌,肝細胞%腫瘤睪汍抗原%診斷
암,간세포%종류고환항원%진단
Carcinoma,hepatocellular%Cancer-testis antigen%Diagnosis
目的 探讨肿瘤睾丸(CT)抗原MAGE-1、SSX-1及CTp11基因mRNA的小肝癌患者外周血中的表达及诊断价值.方法 用巢式逆转录-聚合酶链反应(RT-PCR)法检测30例小肝癌患者外周血单个核细胞(PBMC)中上述3种基因的表达.结果 小肝癌患者PBMC中MAGE-1、SSX-1和CTp11基因mRNA的阳性表达率分别为26.6%(6/30)、26.6%(6/30)和16.6% (5/30),有40.0% (12/30)患者PBMC标本中可以检测到其中1种CT抗原基因表达.在血清甲胎蛋白(AFP)阴性及低浓度阳性小肝癌患者PBMC中其阳性率为58.7% (9/24),此结果与血清AFP检测结果联合起来,可显著提高单纯根据AFP检测结果所做出的诊断率(50.0%比20.0%,P<0.05).结论 外周血中MAGE-1、SSX-1及CTp11基因mRNA的检测有助于小肝癌的诊断.
目的 探討腫瘤睪汍(CT)抗原MAGE-1、SSX-1及CTp11基因mRNA的小肝癌患者外週血中的錶達及診斷價值.方法 用巢式逆轉錄-聚閤酶鏈反應(RT-PCR)法檢測30例小肝癌患者外週血單箇覈細胞(PBMC)中上述3種基因的錶達.結果 小肝癌患者PBMC中MAGE-1、SSX-1和CTp11基因mRNA的暘性錶達率分彆為26.6%(6/30)、26.6%(6/30)和16.6% (5/30),有40.0% (12/30)患者PBMC標本中可以檢測到其中1種CT抗原基因錶達.在血清甲胎蛋白(AFP)陰性及低濃度暘性小肝癌患者PBMC中其暘性率為58.7% (9/24),此結果與血清AFP檢測結果聯閤起來,可顯著提高單純根據AFP檢測結果所做齣的診斷率(50.0%比20.0%,P<0.05).結論 外週血中MAGE-1、SSX-1及CTp11基因mRNA的檢測有助于小肝癌的診斷.
목적 탐토종류고환(CT)항원MAGE-1、SSX-1급CTp11기인mRNA적소간암환자외주혈중적표체급진단개치.방법 용소식역전록-취합매련반응(RT-PCR)법검측30례소간암환자외주혈단개핵세포(PBMC)중상술3충기인적표체.결과 소간암환자PBMC중MAGE-1、SSX-1화CTp11기인mRNA적양성표체솔분별위26.6%(6/30)、26.6%(6/30)화16.6% (5/30),유40.0% (12/30)환자PBMC표본중가이검측도기중1충CT항원기인표체.재혈청갑태단백(AFP)음성급저농도양성소간암환자PBMC중기양성솔위58.7% (9/24),차결과여혈청AFP검측결과연합기래,가현저제고단순근거AFP검측결과소주출적진단솔(50.0%비20.0%,P<0.05).결론 외주혈중MAGE-1、SSX-1급CTp11기인mRNA적검측유조우소간암적진단.
Objective To evaluate the diagnostic value of cancer-testis antigen (CTA) mRNA in peripheral blood samples from patients with small hepatocellular carcinoma (HCC).Methods Peripheral blood samples were drawn from 30 patients with small HCC before operation.The expression of the above CTA genes in the peripheral blood mononuclear cells (PBMCs) was detected by using nested reverse transcriptase-polymerase chain reaction (RT-PCR),and serum alpha-fetoprotein (AFP) levels were also determined.Results Of the 30 patients,the positive rate of MAGE-1,SSX-1 and CTp11 transcripts was 26.6% (6/30),26.6% (6/30) and 16.6% (5/30) in PBMCs,respectively.The positive rate for at least one of the three CTA gene transcripts was 40.0% (12/30) in PBMCs of these patients.MAGE-1,SSX-1 and/or CTp11 mRNA were not detected in the PBMCs of those patients from whom the resected tumor sampies were negative for MAGE-1,SSX-1 and/or CTp11 mRNA,none in the PBMCs from 15 healthy donors and 15 cirrhotic patients.Among the 30 patients,there were only 6 patients whose serum AFP met the general diagnostic standard (AFP > 400 μg/L) for HCC,whereas the other patients were negative for serum AFP (AFP ≤ 20 μg/L) or AFP positive with low concentration (20 μg/L < AFP≤400 pg/L).The positive rate for at least one of the three CTA gene transcripts in PBMCs from the AFP negative patients or the AFP positive patients with low concentration was 33.3% (5/15) and 44.4% (4/9),respectively.50.0% (15/30) of the patients with small HCC could be diagnosed by combining the results of the nested RT-PCR and the serum AFP detection,and this diagnostic rate was significantly higher than that (20.0%)made by AFP detection alone (P < 0.05).Conclusion Detecting MAGE-1,SSX-1 and CTp11 mRNA in PBMCs is va1uable for diagnosing the patients with small HCC.